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It is not due to c-Jun but appears to be related to c-Jun-like neoepitopes generated during apoptosis. This study was planned to gain further information about c-Jun-like immunostaining during apoptosis and to evaluate these antibodies as possible tools for characterizing cell death. Most of the experiments were performed in chick embryo spinal cord. When the apoptotic c-Jun-like immunoreactivity and caspase-3 immunostaining patterns were compared, we found that both antibodies immunostained the same dying cells in a similar pattern. In contrast to TUNEL staining, which reveals a positive reaction in both apoptotic and necrotic dying cells, active caspase-3 and c-Jun\/sc45 antibodies are more selective because they stained only apoptotic cells. When cytosolic extracts from normal tissues were digested in vitro with caspase-3, c-Jun\/sc45 immunoreactivity was strongly induced in several proteins, as demonstrated by Western blotting. Similar results were found when normal tissue sections were treated with caspase-3. Our results show that c-Jun\/sc45 antibodies react with neoepitopes generated from cell proteins cleaved by activated caspases during apoptosis. We conclude that c-Jun\/sc45 antibodies may be useful for detecting apoptosis. They can even be used in archival paraffin-embedded tissue samples.<\/jats:p>","DOI":"10.1177\/002215540205000710","type":"journal-article","created":{"date-parts":[[2011,4,1]],"date-time":"2011-04-01T03:17:54Z","timestamp":1301627874000},"page":"961-972","source":"Crossref","is-referenced-by-count":10,"title":["c-Jun-like Immunoreactivity in Apoptosis Is the Result of a Crossreaction with Neoantigenic Sites Exposed by Caspase-3-mediated Proteolysis"],"prefix":"10.1177","volume":"50","author":[{"given":"Joan","family":"Ribera","sequence":"first","affiliation":[{"name":"Unitat de Neurobiologia Cellular, Departament de Ci\u00e8ncies M\u00e8diques B\u00e1siques, Facultat de Medicina, Lleida, Catalunya, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Victoria","family":"Ayala","sequence":"additional","affiliation":[{"name":"Unitat de Neurobiologia Cellular, Departament de Ci\u00e8ncies M\u00e8diques B\u00e1siques, Facultat de Medicina, Lleida, Catalunya, 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