{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T18:11:53Z","timestamp":1791137513056,"version":"4.1.0"},"reference-count":38,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[1997,3,1]],"date-time":"1997-03-01T00:00:00Z","timestamp":857174400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Histochem Cytochem."],"published-print":{"date-parts":[[1997,3]]},"abstract":"<jats:p>We used riboprobes and monoclonal antibodies to characterize tissue distribution of the human 550-kD homologue to gp330\/megalin, primarily identified in the rat kidney. Human gp330\/megalin mRNA and protein are readily identified in human parathyroid cells, placental cytotrophoblasts, kidney proximal tubule cells, and epididymal epithelial cells. The immunoreactivity is found on the surface of the cells and is heterogeneously downregulated in parathyroid hyperplasia and adenomas. Cells of the proximal kidney tubule and epididymis express the protein on their luminal aspect. Moreover, the protein is expressed in Type II pneumocytes, mammary epithelial and thyroid follicular cells, and the ciliary body of the eye. Sequence analysis of cDNA fragments, obtained by RT-PCR, revealed identical nucleotide sequences in parathyroid, kidney, placenta, epididymis, and lung. Immunohistochemistry for parathyroid hormone-related protein (PTHrP) revealed partial co-expression with human gp330\/megalin in parathyroid, placenta, and mammary gland. The findings substantiate human gp330\/megalin expression in a variety of human tissues expected to possess calcium-sensing functions. It may constitute a protein of utmost importance to adult and fetal calcium homeostasis, although other important functions may also be coupled to this exceptionally large protein with highly restricted tissue distribution.<\/jats:p>\n                  <jats:p>(J Histochem Cytochem 45:383\u2013392, 1997)<\/jats:p>","DOI":"10.1177\/002215549704500306","type":"journal-article","created":{"date-parts":[[2011,4,1]],"date-time":"2011-04-01T03:01:27Z","timestamp":1301626887000},"page":"383-392","source":"Crossref","is-referenced-by-count":162,"title":["Tissue Distribution of Human gp330\/Megalin, a Putative Ca\n                    <sup>2+<\/sup>\n                    -sensing Protein"],"prefix":"10.1177","volume":"45","author":[{"given":"Stefan","family":"Lundgren","sequence":"first","affiliation":[{"name":"Department of Cell Research, Swedish University of Agricultural Science (SL,UP), and Departments of Medical Chemistry (GH,LR) and Surgery (TC,CJ,JR,G\u00c5,PH), Uppsala University, Uppsala, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tobias","family":"Carling","sequence":"additional","affiliation":[{"name":"Department of Cell Research, Swedish University of Agricultural Science (SL,UP), and Departments of Medical Chemistry (GH,LR) and Surgery (TC,CJ,JR,G\u00c5,PH), Uppsala University, Uppsala, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G\u00f6ran","family":"Hj\u00e4lm","sequence":"additional","affiliation":[{"name":"Department of Cell Research, Swedish University of Agricultural Science (SL,UP), and Departments of Medical Chemistry (GH,LR) and Surgery (TC,CJ,JR,G\u00c5,PH), Uppsala University, Uppsala, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claes","family":"Juhlin","sequence":"additional","affiliation":[{"name":"Department of Cell Research, Swedish University of Agricultural Science (SL,UP), and Departments of Medical Chemistry (GH,LR) and Surgery (TC,CJ,JR,G\u00c5,PH), Uppsala University, Uppsala, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonas","family":"Rastad","sequence":"additional","affiliation":[{"name":"Department of Cell Research, Swedish University of Agricultural Science (SL,UP), and Departments of Medical Chemistry (GH,LR) and Surgery (TC,CJ,JR,G\u00c5,PH), Uppsala University, Uppsala, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulla","family":"Pihlgren","sequence":"additional","affiliation":[{"name":"Department of Cell Research, Swedish University of Agricultural Science (SL,UP), and Departments of Medical Chemistry (GH,LR) and Surgery (TC,CJ,JR,G\u00c5,PH), Uppsala University, Uppsala, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lars","family":"Rask","sequence":"additional","affiliation":[{"name":"Department of Cell Research, Swedish University of Agricultural Science (SL,UP), and Departments of Medical Chemistry (GH,LR) and Surgery (TC,CJ,JR,G\u00c5,PH), Uppsala University, Uppsala, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G\u00f6ran","family":"\u00c5kerstr\u00f6m","sequence":"additional","affiliation":[{"name":"Department of Cell Research, Swedish University of Agricultural Science (SL,UP), and Departments of Medical Chemistry (GH,LR) and Surgery (TC,CJ,JR,G\u00c5,PH), Uppsala University, Uppsala, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Per","family":"Hellman","sequence":"additional","affiliation":[{"name":"Department of Cell Research, Swedish University of Agricultural Science (SL,UP), and Departments of Medical Chemistry (GH,LR) and Surgery (TC,CJ,JR,G\u00c5,PH), Uppsala University, Uppsala, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[1997,3,1]]},"reference":[{"key":"bibr1-002215549704500306","first-page":"15","volume-title":"The Parathyroids","author":"Brown EM","year":"1994"},{"key":"bibr2-002215549704500306","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1991.71.2.371"},{"key":"bibr3-002215549704500306","doi-asserted-by":"publisher","DOI":"10.1016\/0143-4160(90)90035-S"},{"key":"bibr4-002215549704500306","doi-asserted-by":"publisher","DOI":"10.1038\/366575a0"},{"key":"bibr5-002215549704500306","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.18.7183"},{"key":"bibr6-002215549704500306","doi-asserted-by":"publisher","DOI":"10.1177\/40.10.1382088"},{"key":"bibr7-002215549704500306","first-page":"1","volume-title":"Plant Molecular Biology: A Practical Approach","author":"Cox HK","year":"1988"},{"key":"bibr8-002215549704500306","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1994.tb44304.x"},{"key":"bibr9-002215549704500306","first-page":"5","volume-title":"Hormones and Cell Regulation","author":"Gylfe E","year":"1990"},{"key":"bibr10-002215549704500306","first-page":"33","volume-title":"Calcium Regulation and Bone Metabolism: Basic and Clinical Aspects","author":"Gylfe E","year":"1987"},{"key":"bibr11-002215549704500306","first-page":"S382","volume":"10","author":"Hastings RH","year":"1995","journal-title":"J Bone Miner Res"},{"key":"bibr12-002215549704500306","doi-asserted-by":"publisher","DOI":"10.1007\/BF02915391"},{"key":"bibr13-002215549704500306","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1993.1603"},{"key":"bibr14-002215549704500306","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(92)90381-6"},{"key":"bibr15-002215549704500306","doi-asserted-by":"publisher","DOI":"10.3109\/02713689508999921"},{"key":"bibr16-002215549704500306","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.9.2990"},{"key":"bibr17-002215549704500306","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(87)91391-X"},{"key":"bibr18-002215549704500306","doi-asserted-by":"publisher","DOI":"10.1210\/endo-122-6-2999"},{"key":"bibr19-002215549704500306","doi-asserted-by":"crossref","first-page":"8275","DOI":"10.1016\/S0021-9258(19)39068-4","volume":"265","author":"Juhlin C","year":"1990","journal-title":"J Biol Chem"},{"key":"bibr20-002215549704500306","first-page":"321","volume":"135","author":"Juhlin C","year":"1989","journal-title":"Am J Pathol"},{"key":"bibr21-002215549704500306","first-page":"1598","volume":"81","author":"Kifor O","year":"1996","journal-title":"Endocrinology"},{"key":"bibr22-002215549704500306","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.22.13070"},{"key":"bibr23-002215549704500306","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1994.1153"},{"key":"bibr24-002215549704500306","volume-title":"Molecular Cloning: A Laboratory Manual","author":"Maniatis T","year":"1982"},{"key":"bibr25-002215549704500306","doi-asserted-by":"publisher","DOI":"10.1210\/endo-128-6-3005"},{"key":"bibr26-002215549704500306","doi-asserted-by":"crossref","first-page":"5188","DOI":"10.1016\/S0021-9258(18)61172-X","volume":"262","author":"Nemeth E","year":"1987","journal-title":"J Biol Chem"},{"key":"bibr27-002215549704500306","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4889(88)90014-6"},{"key":"bibr28-002215549704500306","doi-asserted-by":"publisher","DOI":"10.1126\/science.2786251"},{"key":"bibr29-002215549704500306","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.1170261"},{"key":"bibr30-002215549704500306","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4889(94)90077-9"},{"key":"bibr31-002215549704500306","first-page":"253","volume":"94","author":"Rudberg C","year":"1986","journal-title":"Acta Pathol Microbiol Immunol Scand [A]"},{"key":"bibr32-002215549704500306","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.21.9725"},{"key":"bibr33-002215549704500306","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.5650050810"},{"key":"bibr34-002215549704500306","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.9.2989"},{"key":"bibr35-002215549704500306","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.1.10.993"},{"key":"bibr36-002215549704500306","doi-asserted-by":"crossref","unstructured":"Williams SE, Kounnas MZ, Argraves WS, Strickland DK (1994) The \u03b12-macroglobulin receptor\/low density lipoprotein receptor-related protein and the receptor-associated protein. In Borth W, Feinman RD, Gonias SL, Quigley JP, Strickland DK, eds. Biology of \u03b12-macroglobulin, Its Receptor and Related Proteins Ann NY Acad Sci 1\u201326.","DOI":"10.1111\/j.1749-6632.1994.tb44297.x"},{"key":"bibr37-002215549704500306","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1996.tb00623.x"},{"key":"bibr38-002215549704500306","doi-asserted-by":"publisher","DOI":"10.1177\/42.4.7510321"}],"container-title":["Journal of Histochemistry &amp; Cytochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/002215549704500306","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/002215549704500306","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/002215549704500306","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T22:24:41Z","timestamp":1777501481000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/002215549704500306"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,3]]},"references-count":38,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1997,3]]}},"alternative-id":["10.1177\/002215549704500306"],"URL":"https:\/\/doi.org\/10.1177\/002215549704500306","relation":{},"ISSN":["0022-1554","1551-5044"],"issn-type":[{"value":"0022-1554","type":"print"},{"value":"1551-5044","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,3]]}}}