{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T14:12:58Z","timestamp":1791295978788,"version":"4.1.0"},"reference-count":52,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[1999,4,1]],"date-time":"1999-04-01T00:00:00Z","timestamp":922924800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Histochem Cytochem."],"published-print":{"date-parts":[[1999,4]]},"abstract":"<jats:p>Endothelin-converting enzyme-1 (ECE-1) is the key enzyme of endothelin biosynthesis, catalyzing the final processing step. As shown by the targeted disruption of the ECE-1 gene, mature endothelins must be produced at specific sites for normal embryonic development. Therefore, it is important to know the exact pattern of ECE-1 gene expression. In this study we investigated the cellular distribution of ECE-1 in a variety of human tissues by in situ hybridization and immunohistochemistry. Widespread expression of the ECE-1 gene was noted, with a similar distribution pattern for mRNA and protein in normal human tissues, suggesting a major biological role for ECE-1. ECE-1 levels were particularly high in the cardiovascular, reproductive, and endocrine systems. There was strong and consistent labeling for ECE-1 in the vascular endothelial cells of all organs examined and in various nonvascular cells, especially some glandular cells. A large amount of ECE-1 protein and mRNA was detected in the Leydig cells of the testis and in the granulosa and theca cells of the ovary. In the adrenal gland, ECE-1 was detected in the cortex and medulla, with the strongest labeling in the zona glomerulosa. Therefore, ECE-1 may be involved in other systems, such as the regulation of hormone secretion, rather than exclusively generating ET-1 from its precursor. These results point out the potential side effects of ECE-1 inhibitors that are currently under development for treatment of cardiovascular diseases.<\/jats:p>","DOI":"10.1177\/002215549904700403","type":"journal-article","created":{"date-parts":[[2011,4,1]],"date-time":"2011-04-01T03:11:07Z","timestamp":1301627467000},"page":"447-461","source":"Crossref","is-referenced-by-count":73,"title":["Cellular Distribution of Endothelin-converting Enzyme-1 in Human Tissues"],"prefix":"10.1177","volume":"47","author":[{"given":"Petra","family":"Korth","sequence":"first","affiliation":[{"name":"INSERM U36, Coll\u00e8ge de France, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rainer M.","family":"Bohle","sequence":"additional","affiliation":[{"name":"Institute of Pathology, University of Giessen, Giessen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Corvol","sequence":"additional","affiliation":[{"name":"INSERM U36, 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