{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T23:43:44Z","timestamp":1777851824049,"version":"3.51.4"},"reference-count":68,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[1999,4,1]],"date-time":"1999-04-01T00:00:00Z","timestamp":922924800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Histochem Cytochem."],"published-print":{"date-parts":[[1999,4]]},"abstract":"<jats:p>\n                    Thrombospondin-1 (TSP-1) and an alternatively spliced fibronectin (Fn)-EIIIA isoform are adhesive proteins associated with embryogenesis and tissue remodeling. We compared, by immunohistochemistry and in situ hybridization, the course of TSP-1 and Fn-EIIIA expression in a model of glomerulonephritis induced by Habu snake venom (HV) and characterized by mesangial cell migration, proliferation, and extracellular matrix (ECM) synthesis. At 24 hr after HV, TSP-1 and Fn-EIIIA proteins localized in the central aspects of lesions associated with platelets and macrophages and at the margins of lesions coinciding with mesangial cell migration (determined by Thy-1 staining). Mesangial cells at this time expressed TSP-1 but not Fn-EIIIA mRNA. TSP-1 protein and mRNA peaked in lesions at 48 hr and were associated with cell proliferation (determined by PCNA, \u03b1-smooth muscle actin phenotype, and expression of \u03b2-PDGF receptor mRNA). TSP-1 expression declined at 72 hr when expression of ECM synthesis peaked, as determined by increased expression of collagen Type IV, laminin, and TGF-\u03b2\n                    <jats:sub>1<\/jats:sub>\n                    protein and mRNA. Mesangial cell expression of Fn-EIIIA was first observed at 48 hr and was most abundant at 72 hr after HV. Therefore, platelet-and macrophage-derived Fn-EIIIA and TSP-1 in early lesions are associated with mesangial cell migration. Mesangial cell upregulation of TSP-1 is associated with migration and proliferation but not maximal ECM accumulation, whereas mesangial cell expression of Fn-EIIIA is associated with proliferation and ECM accumulation. These results suggest distinctive temporal and spatial roles for TSP-1 and Fn-EIIIA in remodeling during glomerular disease.\n                  <\/jats:p>","DOI":"10.1177\/002215549904700412","type":"journal-article","created":{"date-parts":[[2011,4,1]],"date-time":"2011-04-01T03:11:07Z","timestamp":1301627467000},"page":"533-543","source":"Crossref","is-referenced-by-count":11,"title":["Differential Expression of Thrombospondin and Cellular Fibronectin During Remodeling in Proliferative Glomerulonephritis"],"prefix":"10.1177","volume":"47","author":[{"given":"Jeffrey L.","family":"Barnes","sequence":"first","affiliation":[{"name":"The Medical Research Service, Audie Murphy Memorial Veterans Administration Hospital, Philadelphia, Pennsylvania"},{"name":"the Department of Medicine, Division of Nephrology, The University of Texas Health Science Center, Philadelphia, Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ronda J.","family":"Mitchell","sequence":"additional","affiliation":[{"name":"the Department of Medicine, Division of Nephrology, The University of Texas Health Science Center, Philadelphia, Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John J.","family":"Kanalas","sequence":"additional","affiliation":[{"name":"San Antonio, Texas, and the Department of Medicine, Division of Nephrology, Thomas Jefferson University, Philadelphia, Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Veronique L.","family":"Barnes","sequence":"additional","affiliation":[{"name":"the Department of Medicine, Division of Nephrology, The University of Texas Health Science Center, Philadelphia, Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[1999,4,1]]},"reference":[{"key":"bibr1-002215549904700412","doi-asserted-by":"publisher","DOI":"10.1084\/jem.151.3.602"},{"key":"bibr2-002215549904700412","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1992.173"},{"key":"bibr3-002215549904700412","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(88)80580-5"},{"key":"bibr4-002215549904700412","doi-asserted-by":"publisher","DOI":"10.1177\/37.7.2659663"},{"key":"bibr5-002215549904700412","first-page":"1366","volume":"143","author":"Barnes JL","year":"1993","journal-title":"Am J Pathol"},{"key":"bibr6-002215549904700412","first-page":"585","volume":"145","author":"Barnes JL","year":"1994","journal-title":"Am J Pathol"},{"key":"bibr7-002215549904700412","first-page":"859","volume":"138","author":"Barnes JL","year":"1991","journal-title":"Am J Pathol"},{"key":"bibr8-002215549904700412","first-page":"460","volume":"70","author":"Barnes JL","year":"1994","journal-title":"Lab Invest"},{"key":"bibr9-002215549904700412","doi-asserted-by":"publisher","DOI":"10.1177\/43.9.7642963"},{"key":"bibr10-002215549904700412","first-page":"1361","volume":"147","author":"Barnes JL","year":"1995","journal-title":"Am J Pathol"},{"key":"bibr11-002215549904700412","doi-asserted-by":"publisher","DOI":"10.1016\/0922-3371(90)90059-6"},{"key":"bibr12-002215549904700412","first-page":"793","volume":"142","author":"Brown LF","year":"1993","journal-title":"Am J Pathol"},{"key":"bibr13-002215549904700412","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1989.70"},{"key":"bibr14-002215549904700412","first-page":"1851","volume":"148","author":"DiPietro LA","year":"1996","journal-title":"Am J Pathol"},{"key":"bibr15-002215549904700412","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1992.104"},{"key":"bibr16-002215549904700412","first-page":"20","volume":"61","author":"Feinberg RF","year":"1989","journal-title":"Lab Invest"},{"key":"bibr17-002215549904700412","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1242\/dev.104.3.369","volume":"104","author":"Ffrench-Constant C","year":"1988","journal-title":"Development"},{"key":"bibr18-002215549904700412","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1242\/dev.106.2.375","volume":"106","author":"Ffrench-Constant C","year":"1989","journal-title":"Development"},{"key":"bibr19-002215549904700412","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.109.2.903"},{"key":"bibr20-002215549904700412","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.105.2.625"},{"key":"bibr21-002215549904700412","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.17.6624"},{"key":"bibr22-002215549904700412","doi-asserted-by":"crossref","first-page":"11289","DOI":"10.1016\/S0021-9258(19)38589-8","volume":"265","author":"Harpel PC","year":"1990","journal-title":"J Biol Chem"},{"key":"bibr23-002215549904700412","doi-asserted-by":"publisher","DOI":"10.1021\/bi00116a036"},{"key":"bibr24-002215549904700412","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1995.483"},{"key":"bibr25-002215549904700412","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4612-3264-3"},{"key":"bibr26-002215549904700412","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90115-S"},{"key":"bibr27-002215549904700412","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90472-O"},{"key":"bibr28-002215549904700412","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)35666-1"},{"key":"bibr29-002215549904700412","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.11.5026"},{"key":"bibr30-002215549904700412","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001970105"},{"key":"bibr31-002215549904700412","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V65.1.79.79"},{"key":"bibr32-002215549904700412","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115089"},{"key":"bibr33-002215549904700412","first-page":"68","volume":"69","author":"Kagami S","year":"1993","journal-title":"Lab Invest"},{"key":"bibr34-002215549904700412","doi-asserted-by":"publisher","DOI":"10.1021\/bi00374a014"},{"key":"bibr35-002215549904700412","first-page":"24","volume":"5","author":"Kobayashi S","year":"1991","journal-title":"J Diabetes Complications"},{"key":"bibr36-002215549904700412","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.107.6.2351"},{"key":"bibr37-002215549904700412","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1989.68"},{"key":"bibr38-002215549904700412","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.106.2.415"},{"key":"bibr39-002215549904700412","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1994.281"},{"key":"bibr40-002215549904700412","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cb.04.110188.001151"},{"key":"bibr41-002215549904700412","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1983.119"},{"key":"bibr42-002215549904700412","doi-asserted-by":"publisher","DOI":"10.1038\/367193a0"},{"key":"bibr43-002215549904700412","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(86)90220-9"},{"key":"bibr44-002215549904700412","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.107.6.2737"},{"key":"bibr45-002215549904700412","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.111.6.2713"},{"key":"bibr46-002215549904700412","doi-asserted-by":"crossref","first-page":"12258","DOI":"10.1016\/S0021-9258(18)67233-3","volume":"261","author":"Paul JI","year":"1986","journal-title":"J Biol Chem"},{"key":"bibr47-002215549904700412","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041530117"},{"key":"bibr48-002215549904700412","doi-asserted-by":"publisher","DOI":"10.3109\/15419069509081278"},{"key":"bibr49-002215549904700412","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(89)92098-6"},{"key":"bibr50-002215549904700412","doi-asserted-by":"publisher","DOI":"10.1172\/JCI109334"},{"key":"bibr51-002215549904700412","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/18.10.3059"},{"key":"bibr52-002215549904700412","first-page":"364","volume":"129","author":"Raugi GJ","year":"1987","journal-title":"Am J Pathol"},{"key":"bibr53-002215549904700412","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12461198"},{"key":"bibr54-002215549904700412","doi-asserted-by":"publisher","DOI":"10.1177\/41.10.8245406"},{"key":"bibr55-002215549904700412","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1990.tb17931.x"},{"key":"bibr56-002215549904700412","doi-asserted-by":"crossref","first-page":"14831","DOI":"10.1016\/S0021-9258(18)98545-5","volume":"266","author":"Sage H","year":"1991","journal-title":"J Biol Chem"},{"key":"bibr57-002215549904700412","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.109.5.2471"},{"key":"bibr58-002215549904700412","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.4.935"},{"key":"bibr59-002215549904700412","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001990302"},{"key":"bibr60-002215549904700412","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(89)90346-7"},{"key":"bibr61-002215549904700412","doi-asserted-by":"publisher","DOI":"10.1016\/0046-8177(93)90066-P"},{"key":"bibr62-002215549904700412","first-page":"566","volume":"67","author":"Taraboletti G","year":"1992","journal-title":"Lab Invest"},{"key":"bibr63-002215549904700412","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.111.2.765"},{"key":"bibr64-002215549904700412","doi-asserted-by":"publisher","DOI":"10.1002\/bies.950180113"},{"key":"bibr65-002215549904700412","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115405"},{"key":"bibr66-002215549904700412","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041570104"},{"key":"bibr67-002215549904700412","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.5.1814"},{"key":"bibr68-002215549904700412","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1994.122"}],"container-title":["Journal of Histochemistry &amp; Cytochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/002215549904700412","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/002215549904700412","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/002215549904700412","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T22:26:44Z","timestamp":1777501604000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/002215549904700412"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,4]]},"references-count":68,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1999,4]]}},"alternative-id":["10.1177\/002215549904700412"],"URL":"https:\/\/doi.org\/10.1177\/002215549904700412","relation":{},"ISSN":["0022-1554","1551-5044"],"issn-type":[{"value":"0022-1554","type":"print"},{"value":"1551-5044","type":"electronic"}],"subject":[],"published":{"date-parts":[[1999,4]]}}}