{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T02:49:52Z","timestamp":1793674192756,"version":"4.5.1"},"reference-count":26,"publisher":"SAGE Publications","issue":"11","license":[{"start":{"date-parts":[[1999,11,1]],"date-time":"1999-11-01T00:00:00Z","timestamp":941414400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Histochem Cytochem."],"published-print":{"date-parts":[[1999,11]]},"abstract":"<jats:p>Airway epithelia play a crucial role in protecting the lung from the external environment. Ciliated airway epithelial cells contribute to mucociliary transport systems via ciliary beating and electrolyte transport mechanisms to defend against respiratory tract infection. Both of these activities are regulated by nitric oxide (NO)-dependent mechanisms. To better understand the role of the NO-cGMP signal transduction cascade in these responses, we investigated the localization of endothelial nitric oxide synthase (eNOS), soluble guanylyl cyclase (sGC), cGMP-dependent protein kinase (PKG) I-\u03b1, and PKG I-\u03b2 in the tracheas and lungs of normal rats by immunohistochemistry. Mouse anti-eNOS, rabbit anti-sGC, PKG I-\u03b1, and PKG I-\u03b2 antibodies were used. Strong immunostaining for eNOS was detected in ciliated tracheal, bronchial, and bronchiolar epithelia, in Clara cells, and in Type II alveolar cells. The pattern of sGC and PKG I-\u03b2 immunostaining showed striking parallels with that of eNOS staining. No staining was detectable in ciliated epithelium with the anti-PKG I-\u03b1 antibody. Taken together, these observations suggest that PKG I-\u03b2 might transduce NO-sGC signaling into biological responses in ciliated respiratory epithelia.<\/jats:p>","DOI":"10.1177\/002215549904701103","type":"journal-article","created":{"date-parts":[[2011,4,1]],"date-time":"2011-04-01T03:11:07Z","timestamp":1301627467000},"page":"1369-1374","source":"Crossref","is-referenced-by-count":31,"title":["Immunohistochemical Evidence for the NO cGMP Signaling Pathway In Respiratory Ciliated Epithelia of Rat"],"prefix":"10.1177","volume":"47","author":[{"given":"Xinhua","family":"Zhan","sequence":"first","affiliation":[{"name":"Department of Anesthesiology, University of Virginia Health System, Charlottesville, Virginia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dechun","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, University of Virginia Health System, Charlottesville, Virginia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roger A.","family":"Johns","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, University of Virginia Health System, Charlottesville, Virginia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[1999,11,1]]},"reference":[{"key":"bibr1-002215549904701103","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.78.7.4241"},{"key":"bibr2-002215549904701103","doi-asserted-by":"publisher","DOI":"10.1172\/JCI1378"},{"key":"bibr3-002215549904701103","doi-asserted-by":"publisher","DOI":"10.1172\/JCI118837"},{"key":"bibr4-002215549904701103","first-page":"538","volume":"149","author":"Gaston B","year":"1994","journal-title":"Am J Respir Cell Mol Biol"},{"key":"bibr5-002215549904701103","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(91)91268-H"},{"key":"bibr6-002215549904701103","doi-asserted-by":"publisher","DOI":"10.1126\/science.274.5288.774"},{"key":"bibr7-002215549904701103","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1993.1187"},{"key":"bibr8-002215549904701103","first-page":"L911","volume":"268","author":"Jain B","year":"1995","journal-title":"Am J Physiol"},{"key":"bibr9-002215549904701103","doi-asserted-by":"publisher","DOI":"10.3109\/00016489709113468"},{"key":"bibr10-002215549904701103","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.94.07081501"},{"key":"bibr11-002215549904701103","doi-asserted-by":"publisher","DOI":"10.1038\/358676a0"},{"key":"bibr12-002215549904701103","doi-asserted-by":"publisher","DOI":"10.1016\/S1054-3589(08)60049-6"},{"key":"bibr13-002215549904701103","doi-asserted-by":"crossref","first-page":"16841","DOI":"10.1016\/S0021-9258(17)44837-X","volume":"265","author":"Nakane M","year":"1989","journal-title":"J Biol Chem"},{"key":"bibr14-002215549904701103","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.me.35.020184.002405"},{"key":"bibr15-002215549904701103","doi-asserted-by":"publisher","DOI":"10.1002\/jlb.57.4.670"},{"key":"bibr16-002215549904701103","first-page":"L704","volume":"267","author":"Rengasamy A","year":"1994","journal-title":"Am J Physiol"},{"key":"bibr17-002215549904701103","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(89)81114-7"},{"key":"bibr18-002215549904701103","doi-asserted-by":"publisher","DOI":"10.1002\/cm.970140305"},{"key":"bibr19-002215549904701103","doi-asserted-by":"publisher","DOI":"10.1172\/JCI117538"},{"key":"bibr20-002215549904701103","doi-asserted-by":"crossref","first-page":"584","DOI":"10.1016\/S0022-3565(25)10694-0","volume":"274","author":"Takemura H","year":"1995","journal-title":"J Pharmacol Exp Ther"},{"key":"bibr21-002215549904701103","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1999.179aa.x"},{"key":"bibr22-002215549904701103","doi-asserted-by":"publisher","DOI":"10.1002\/cm.970270202"},{"key":"bibr23-002215549904701103","doi-asserted-by":"publisher","DOI":"10.1016\/S0074-7696(08)61970-1"},{"key":"bibr24-002215549904701103","first-page":"L827","volume":"275","author":"Wyatt TA","year":"1998","journal-title":"Am J Physiol"},{"key":"bibr25-002215549904701103","doi-asserted-by":"publisher","DOI":"10.1177\/44.5.8627003"},{"key":"bibr26-002215549904701103","doi-asserted-by":"publisher","DOI":"10.1177\/000348949710600309"}],"container-title":["Journal of Histochemistry &amp; 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