{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T17:50:35Z","timestamp":1790272235979,"version":"4.1.0"},"reference-count":7,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2013,7,4]],"date-time":"2013-07-04T00:00:00Z","timestamp":1372896000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Lab Anim"],"published-print":{"date-parts":[[2013,10]]},"abstract":"<jats:p>Repeated blood sampling from laboratory animals is desirable in certain experimental designs and also for reducing the number of animals used in research. Biochemical methods for analysing blood samples require only small blood volumes to be collected (typically 20\u201340\u2009\u00b5L). In juvenile mice, the small blood volume of the animals also requires only small samples to be taken. Furthermore, for behavioural studies it is desirable to have a method that does not require anaesthesia or the use of invasive indwelling cannulae. We report the validation of a refined method for repeated blood sampling (up to 3 times at 24\u2009h intervals) in juvenile and adult mice using the tail incision method to sample from the lateral tail vein. This method is not stressful, as assessed by low basal levels of the stress hormone corticosterone. Since repeated blood samples can be collected from the same animal at multiple time points, it is not necessary to increase group size for terminal sample collection. Thus, in addition to being a refined method requiring no warming of the tail, no anaesthesia and only gentle restraint, this method also reduces the numbers of mice used for experiments.<\/jats:p>","DOI":"10.1177\/0023677213494366","type":"journal-article","created":{"date-parts":[[2013,7,5]],"date-time":"2013-07-05T00:24:47Z","timestamp":1372983887000},"page":"316-319","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":34,"title":["Validation of a refined technique for taking repeated blood samples from juvenile and adult mice"],"prefix":"10.1177","volume":"47","author":[{"given":"Annelisa M","family":"Sadler","sequence":"first","affiliation":[{"name":"Department of Pharmacy and Pharmacology, University of Bath, Claverton Down, Bath, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah J","family":"Bailey","sequence":"additional","affiliation":[{"name":"Department of Pharmacy and Pharmacology, University of Bath, Claverton Down, Bath, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2013,7,4]]},"reference":[{"key":"bibr1-0023677213494366","first-page":"47","volume":"29","author":"Hoff J","year":"2000","journal-title":"Lab Anim"},{"key":"bibr2-0023677213494366","unstructured":"National Centre for the Replacement, Refinement and Reduction of Animals in Research. http:\/\/www.nc3rs.org.uk\/bloodsamplingmicrosite\/page.asp?id=364 (accessed 24 February 2013)."},{"key":"bibr3-0023677213494366","volume-title":"Reducing the use of animals in research through better experimental design","author":"Festing MFW","year":"2002"},{"key":"bibr4-0023677213494366","doi-asserted-by":"publisher","DOI":"10.1002\/jat.727"},{"key":"bibr5-0023677213494366","doi-asserted-by":"publisher","DOI":"10.1258\/002367700780387714"},{"key":"bibr6-0023677213494366","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1500"},{"key":"bibr7-0023677213494366","doi-asserted-by":"publisher","DOI":"10.1177\/0269881111420313"}],"container-title":["Laboratory Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0023677213494366","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/0023677213494366","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0023677213494366","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T07:33:39Z","timestamp":1777620819000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/0023677213494366"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,7,4]]},"references-count":7,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2013,10]]}},"alternative-id":["10.1177\/0023677213494366"],"URL":"https:\/\/doi.org\/10.1177\/0023677213494366","relation":{},"ISSN":["0023-6772","1758-1117"],"issn-type":[{"value":"0023-6772","type":"print"},{"value":"1758-1117","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,7,4]]}}}