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The major limiting factor is the duration of the transportation. Previous reports have shown that sperm collected from transported epididymis maintained their fertility for around 72\u2009h, but there are no published data with longer transportation times, and this window of time may be too short, especially for international shipments and where locations are not well connected. In this study live pups were born using frozen\u2013thawed sperm after up to 192\u2009h (8 days) of transportation, using a special in\u00a0vitro fertilization design which resulted in a fertilization rate of 10.5%.<\/jats:p>","DOI":"10.1177\/0023677217692612","type":"journal-article","created":{"date-parts":[[2017,2,7]],"date-time":"2017-02-07T08:08:52Z","timestamp":1486454932000},"page":"526-529","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["Revitalizing genetically-modified mouse strains using frozen\u2013thawed sperm after up to 192\u2009h of refrigerated epididymis transportation"],"prefix":"10.1177","volume":"51","author":[{"given":"Gonzalo","family":"Moreno-del Val","sequence":"first","affiliation":[{"name":"Transgenic and Cryopreservation Laboratory, Servicio de Experimentaci\u00f3n Animal UMH, Instituto de Neurociencias de Alicante, Consejo Superior de Investigaciones Cient\u00edficas \/Universidad Miguel Hern\u00e1ndez, Alicante, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia","family":"Mu\u00f1oz-Robledano","sequence":"additional","affiliation":[{"name":"Transgenic and Cryopreservation Laboratory, Servicio de Experimentaci\u00f3n Animal UMH, Instituto de Neurociencias de Alicante, Consejo Superior de Investigaciones Cient\u00edficas \/Universidad Miguel Hern\u00e1ndez, Alicante, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2017,2,6]]},"reference":[{"key":"bibr1-0023677217692612","doi-asserted-by":"publisher","DOI":"10.1274\/jmor.14.45"},{"key":"bibr2-0023677217692612","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2004.11.013"},{"key":"bibr3-0023677217692612","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2012.06.002"},{"key":"bibr4-0023677217692612","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2009.04.006"},{"key":"bibr5-0023677217692612","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2013.10.007"},{"key":"bibr6-0023677217692612","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(01)00518-0"},{"key":"bibr7-0023677217692612","doi-asserted-by":"publisher","DOI":"10.1002\/9780470942390.mo140064"},{"key":"bibr8-0023677217692612","doi-asserted-by":"publisher","DOI":"10.1258\/la.2009.009074"},{"key":"bibr9-0023677217692612","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.111.092536"},{"key":"bibr10-0023677217692612","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0009387"}],"container-title":["Laboratory Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0023677217692612","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/0023677217692612","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0023677217692612","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T07:36:41Z","timestamp":1777621001000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/0023677217692612"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,2,6]]},"references-count":10,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2017,10]]}},"alternative-id":["10.1177\/0023677217692612"],"URL":"https:\/\/doi.org\/10.1177\/0023677217692612","relation":{},"ISSN":["0023-6772","1758-1117"],"issn-type":[{"value":"0023-6772","type":"print"},{"value":"1758-1117","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,2,6]]}}}