{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T16:07:01Z","timestamp":1783181221615,"version":"3.54.6"},"reference-count":33,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2020,6,6]],"date-time":"2020-06-06T00:00:00Z","timestamp":1591401600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Central Institute for Experimental Animals","award":["Internal grant"],"award-info":[{"award-number":["Internal grant"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Lab Anim"],"published-print":{"date-parts":[[2021,2]]},"abstract":"<jats:p>\n                    Severe immunodeficient mice are an essential tool for the examination of the efficacy and safety of new therapeutic technologies as a humanized model. Previously, non-obese diabetic (NOD)\/Shi-scid IL2r\u03b3\n                    <jats:sup>null<\/jats:sup>\n                    (NOG) mice were established as immunodeficient mice by combining interleukin-2 receptor-\u03b3 chain-knockout mice and NOD\/Shi-scid mice. The NOG mice are used frequently in the research of therapeutic monoclonal antibodies and regenerative medicine for human diseases. Establishment of an efficient production system of NOG mice, using optimized reproductive techniques, is required to accelerate research. In this study, we investigated the efficacy of the superovulation technique using equine chorionic gonadotropin (eCG) and inhibin antiserum (IAS) in NOG mice of various ages (4, 8, 12, 24, or 54 weeks). Additionally, we examined the fertilizing and developmental ability of the oocytes through in-vitro fertilization using frozen\u2013thawed sperm, embryo culture and embryo transfer. The results showed that NOG mice produced the highest number of oocytes at 12 weeks old following the co-administration of eCG and IAS (collectively IASe) (70 oocytes\/female). IASe was more effective in increasing the number of oocytes v. eCG at all ages. The IASe-derived oocytes demonstrated the ability to fertilize and develop into blastocysts and pups. Finally, we demonstrated that three strains of genetically modified NOG mice were efficiently produced through the optimized reproductive techniques. In summary, we developed an efficient system for the production of immunodeficient mice using 12-week-old, IASe-treated female NOG mice.\n                  <\/jats:p>","DOI":"10.1177\/0023677220928091","type":"journal-article","created":{"date-parts":[[2020,6,7]],"date-time":"2020-06-07T00:46:40Z","timestamp":1591490800000},"page":"13-20","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":6,"title":["Efficient production of immunodeficient non-obese diabetic\/Shi-scid\n                    <i>\n                      IL2r\n                      <b>\u03b3<\/b>\n                    <\/i>\n                    <sup>null<\/sup>\n                    mice via the superovulation technique using inhibin antiserum and gonadotropin"],"prefix":"10.1177","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6288-4583","authenticated-orcid":false,"given":"Motohito","family":"Goto","sequence":"first","affiliation":[{"name":"Central Institute for Experimental Animals (CIEA), Japan"},{"name":"Center for Animal Resources and Development (CARD), Kumamoto University, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8103-651X","authenticated-orcid":false,"given":"Toru","family":"Takeo","sequence":"additional","affiliation":[{"name":"Center for Animal Resources and Development (CARD), Kumamoto University, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Riichi","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Central Institute for Experimental Animals (CIEA), Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naomi","family":"Nakagata","sequence":"additional","affiliation":[{"name":"Center for Animal Resources and Development (CARD), Kumamoto University, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2020,6,6]]},"reference":[{"key":"bibr1-0023677220928091","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3954"},{"key":"bibr2-0023677220928091","doi-asserted-by":"publisher","DOI":"10.1158\/2159-8290.CD-14-0001"},{"key":"bibr3-0023677220928091","doi-asserted-by":"publisher","DOI":"10.1038\/nrc.2016.140"},{"key":"bibr4-0023677220928091","unstructured":"Japanese PDX library. 2018. https:\/\/www.ncc.go.jp\/en\/ri\/division\/molecular_pharmacology\/index.html"},{"key":"bibr5-0023677220928091","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2776(08)60490-3"},{"key":"bibr6-0023677220928091","doi-asserted-by":"publisher","DOI":"10.1038\/301527a0"},{"key":"bibr7-0023677220928091","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2001-12-0207"},{"key":"bibr8-0023677220928091","doi-asserted-by":"publisher","DOI":"10.1538\/expanim.17-0034"},{"key":"bibr9-0023677220928091","doi-asserted-by":"publisher","DOI":"10.3390\/jcm4010159"},{"key":"bibr10-0023677220928091","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.107.065359"},{"key":"bibr11-0023677220928091","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.111.092536"},{"key":"bibr12-0023677220928091","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0128330"},{"key":"bibr13-0023677220928091","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2016.04.076"},{"key":"bibr14-0023677220928091","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1000412"},{"key":"bibr15-0023677220928091","doi-asserted-by":"publisher","DOI":"10.1007\/s003350010109"},{"key":"bibr16-0023677220928091","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1985.tb37520.x"},{"key":"bibr17-0023677220928091","doi-asserted-by":"publisher","DOI":"10.1017\/S0967199408004619"},{"key":"bibr18-0023677220928091","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.086967"},{"key":"bibr19-0023677220928091","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0390433"},{"key":"bibr20-0023677220928091","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(93)25012-Q"},{"key":"bibr21-0023677220928091","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2015.01.008"},{"key":"bibr22-0023677220928091","doi-asserted-by":"publisher","DOI":"10.1258\/la.2009.009074"},{"key":"bibr23-0023677220928091","doi-asserted-by":"publisher","DOI":"10.1538\/expanim1978.41.3_387"},{"key":"bibr24-0023677220928091","doi-asserted-by":"publisher","DOI":"10.1538\/expanim.60.481"},{"key":"bibr25-0023677220928091","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2005.09.016"},{"key":"bibr26-0023677220928091","doi-asserted-by":"publisher","DOI":"10.1038\/srep05290"},{"key":"bibr27-0023677220928091","doi-asserted-by":"crossref","unstructured":"Takeo T, Mukunoki A, Nakagata N. Ovulation of juvenile, mature, and aged female C57BL\/6 mice following coadministration of inhibin antiserum and equine chorionic gonadotropin.\n                      Cryobiology\n                      2019; 135: 1\u20136.","DOI":"10.1016\/j.theriogenology.2019.05.028"},{"key":"bibr28-0023677220928091","first-page":"297","volume":"42","author":"Sugiyama F","year":"1992","journal-title":"Lab Anim Sci"},{"key":"bibr29-0023677220928091","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-8831-0_14"},{"key":"bibr30-0023677220928091","doi-asserted-by":"publisher","DOI":"10.1007\/s00335-015-9600-0"},{"key":"bibr31-0023677220928091","doi-asserted-by":"publisher","DOI":"10.1242\/bio.019349"},{"key":"bibr32-0023677220928091","doi-asserted-by":"publisher","DOI":"10.1538\/expanim.18-0062"},{"key":"bibr33-0023677220928091","doi-asserted-by":"publisher","DOI":"10.1242\/bio.025122"}],"container-title":["Laboratory Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0023677220928091","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/0023677220928091","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0023677220928091","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T07:39:47Z","timestamp":1777621187000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/0023677220928091"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6,6]]},"references-count":33,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,2]]}},"alternative-id":["10.1177\/0023677220928091"],"URL":"https:\/\/doi.org\/10.1177\/0023677220928091","relation":{},"ISSN":["0023-6772","1758-1117"],"issn-type":[{"value":"0023-6772","type":"print"},{"value":"1758-1117","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,6,6]]}}}