{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T11:45:27Z","timestamp":1777895127689,"version":"3.51.4"},"reference-count":17,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2024,3,9]],"date-time":"2024-03-09T00:00:00Z","timestamp":1709942400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Lab Anim"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:p>\n                    Staphylococcus aureus nasal carriage is considered a risk factor for infections, and the development of nasal decolonization strategies is highly relevant. Despite they are not naturally colonized by Staphylococcus, mice are a good model for S. aureus nasal colonization. Murine models are easy to manipulate, and inter-laboratory reproducibility makes them suitable for nasal colonization studies. Strategies using bioluminescent bacteria allow for the monitoring of infection over time without the need to sacrifice animals for bacterial quantification. In this study, we evaluated S. aureus nasal colonization in three mouse strains (BALB\/c, C57BL\/6, and Swiss Webster) using a bioluminescent strain (SAP231). In vitro, a visible Bioluminescent Signal Emission (BLSE) was observed until 10\n                    <jats:sup>6<\/jats:sup>\n                    bacteria and detected by IVIS\u00ae imaging system up to 10\n                    <jats:sup>4<\/jats:sup>\n                    cells. Animals were inoculated with one or two doses of approximately 10\n                    <jats:sup>9<\/jats:sup>\n                    colony-forming units (CFU) of SAP231. Swiss Webster mice showed the longest colonization time, with some animals presenting BLSE for up to 140\u2009h. In addition, BLSE was higher in this strain. BALB\/c and C57BL\/6 strains showed consistent BLSE results for 48\u2009h. BLSE intensity was higher in Swiss Webster inoculated with both doses. Three different positions for image capture were evaluated, with better results for the lateral and ventrodorsal positions. After the loss of BLSE, bacterial quantification was performed, and Swiss Webster mice presented more bacteria in the nasal cavity (approximately 10\n                    <jats:sup>5<\/jats:sup>\n                    CFU) than the other strains. Our results demonstrate that bioluminescent S. aureus allow monitoring of nasal colonization and estimation of the bacterial burden present in live animals until 48\u2009h.\n                  <\/jats:p>","DOI":"10.1177\/00236772231209790","type":"journal-article","created":{"date-parts":[[2024,3,9]],"date-time":"2024-03-09T19:06:05Z","timestamp":1710011165000},"page":"231-239","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Monitoring\n                    <i>Staphylococcus aureus<\/i>\n                    nasal colonization murine model using a bioluminescent methicillin-resistant\n                    <i>S. aureus<\/i>\n                    (MRSA)"],"prefix":"10.1177","volume":"58","author":[{"given":"Juliana G da","family":"Silva","sequence":"first","affiliation":[{"name":"FIOCRUZ, Instituto de Tecnologia em Imunobiol\u00f3gicos, Rio de Janeiro, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juliana PC","family":"Boechat","sequence":"additional","affiliation":[{"name":"FIOCRUZ, Instituto de Tecnologia em Imunobiol\u00f3gicos, Rio de Janeiro, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruno DJ","family":"Silva","sequence":"additional","affiliation":[{"name":"FIOCRUZ, Instituto de Tecnologia em Imunobiol\u00f3gicos, Rio de Janeiro, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rodrigo","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"FIOCRUZ, Instituto de Tecnologia em Imunobiol\u00f3gicos, Rio de Janeiro, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0837-7414","authenticated-orcid":false,"given":"Jos\u00e9 PM","family":"Senna","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz, Instituto de Tecnologia emImunobiol\u00f3gicos-BioManguinhos Fiocruz, Rio de Janeiro, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2024,3,9]]},"reference":[{"key":"bibr1-00236772231209790","doi-asserted-by":"publisher","DOI":"10.1080\/21505594.2021.1878688"},{"key":"bibr2-00236772231209790","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(05)70295-4"},{"key":"bibr3-00236772231209790","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-72063-0"},{"key":"bibr4-00236772231209790","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0225752"},{"key":"bibr5-00236772231209790","first-page":"203","volume":"17","author":"Turner NA","year":"2019","journal-title":"Nat Rev"},{"key":"bibr6-00236772231209790","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02423-12"},{"key":"bibr7-00236772231209790","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200101043440102"},{"key":"bibr8-00236772231209790","doi-asserted-by":"publisher","DOI":"10.1086\/599020"},{"key":"bibr9-00236772231209790","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.3.1847-1851.2005"},{"key":"bibr10-00236772231209790","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0059232"},{"key":"bibr11-00236772231209790","doi-asserted-by":"publisher","DOI":"10.1177\/0023677219826165"},{"key":"bibr12-00236772231209790","volume-title":"Animal models for the study of human disease","author":"Conn PM.","year":"2017"},{"key":"bibr13-00236772231209790","doi-asserted-by":"publisher","DOI":"10.1002\/ar.23844"},{"key":"bibr14-00236772231209790","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00097-20"},{"key":"bibr15-00236772231209790","unstructured":"Beynen AC, G\u00e4rtner K, van Zutphen LFM.\n                      Standardization of animal experimentation Principles of laboratory animal science: A contribution to the humane use and care of animals and the quality of experimental results\n                      . 2nd ed.  Amsterdam:  Elsevier Ltd, 2001, pp.103\u2013110."},{"key":"bibr16-00236772231209790","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S120939"},{"key":"bibr17-00236772231209790","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0071142"}],"container-title":["Laboratory Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/00236772231209790","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/00236772231209790","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/00236772231209790","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T07:43:23Z","timestamp":1777621403000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/00236772231209790"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,3,9]]},"references-count":17,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2024,6]]}},"alternative-id":["10.1177\/00236772231209790"],"URL":"https:\/\/doi.org\/10.1177\/00236772231209790","relation":{},"ISSN":["0023-6772","1758-1117"],"issn-type":[{"value":"0023-6772","type":"print"},{"value":"1758-1117","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,3,9]]}}}