{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T04:18:58Z","timestamp":1787545138382,"version":"build-2736575974"},"reference-count":50,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100003710","name":"Korea Health Industry Development Institute","doi-asserted-by":"crossref","award":["RS-2024-00406488"],"award-info":[{"award-number":["RS-2024-00406488"]}],"id":[{"id":"10.13039\/501100003710","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["RS-2024-00456300"],"award-info":[{"award-number":["RS-2024-00456300"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Korea Health Technology R&D Project"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Public Health Rep"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:sec>\n                    <jats:title>Objectives:<\/jats:title>\n                    <jats:p>Zoonotic diseases pose substantial public health risks because of their potential transmission from animals to humans. As pets, cats can harbor these pathogens. The objective of this study was to describe bacterial and eukaryotic pathogens in the feces of stray and pet cats in Seoul, South Korea, using next-generation sequencing techniques.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>\n                      We collected 26 fresh fecal samples (17 from pet cats and 9 from stray cats) in Seoul\u2019s Mapo-gu District in April and May 2022. Amplicon sequencing targeted the V4 region of the 16S rRNA gene for bacterial pathogens and the V9 region of the 18S rRNA gene for eukaryotic pathogens. We used QIIME 2 to conduct bioinformatic analysis, assessing alpha diversity with the Shannon Diversity Index and beta diversity with principal coordinates analysis and permutational multivariate analysis of variance. We used ALDEx2 and an analysis of the composition of microbiomes to analyze differential abundance and \u03c7\n                      <jats:sup>2<\/jats:sup>\n                      tests to assess pathogen prevalence.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      Across all 26 samples,\n                      <jats:italic toggle=\"yes\">Helicobacter<\/jats:italic>\n                      spp (77%; n\u2009=\u200920) and\n                      <jats:italic toggle=\"yes\">Campylobacter<\/jats:italic>\n                      spp (69%; n\u2009=\u200918) were the most prevalent bacterial pathogens.\n                      <jats:italic toggle=\"yes\">Escherichia-Shigella<\/jats:italic>\n                      spp were more common in stray cats (56% [5 of 9]) than in pet cats (12% [2 of 17]) as were\n                      <jats:italic toggle=\"yes\">Brachyspira<\/jats:italic>\n                      spp (stray cats, 44% [4 of 9]; pet cats, 0%). Of eukaryotic pathogens,\n                      <jats:italic toggle=\"yes\">Giardia<\/jats:italic>\n                      spp (19% [5 of 26]) were most prevalent across both groups, with\n                      <jats:italic toggle=\"yes\">Pentatrichomonas<\/jats:italic>\n                      spp significantly more common in stray cats (22% [2 of 9]) than in pet cats (0%).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>This study found distinct fecal microbial communities in stray versus pet cats, with a higher prevalence of potential pathogens in stray cats. These findings emphasize the need for public health planning and effective measures for controlling stray cats.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/00333549251342745","type":"journal-article","created":{"date-parts":[[2025,7,4]],"date-time":"2025-07-04T06:03:45Z","timestamp":1751609025000},"page":"365-372","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":5,"title":["Metabarcoding Study of Fecal Bacterial and Eukaryotic Pathogens in Stray and Pet Cats in Seoul, South Korea, 2022"],"prefix":"10.1177","volume":"140","author":[{"given":"Maria Gloria Ojeda","family":"Ayala","sequence":"first","affiliation":[{"name":"Department of Global Health and Disease Control, Graduate School of Public Health, Yonsei University, Seoul, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-2757-9529","authenticated-orcid":false,"given":"Singeun","family":"Oh","sequence":"additional","affiliation":[{"name":"Department of Tropical Medicine, Institute of Tropical Medicine, Arthropods of Medical Importance Resource Bank, Yonsei University College of Medicine, Seoul, Republic of Korea"},{"name":"Department of Tropical Medicine, Graduate School of Medical Science, Brain Korea 21 Project, Yonsei University College of Medicine, Seoul, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun Ho","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Tropical Medicine, Institute of Tropical Medicine, Arthropods of Medical Importance Resource Bank, Yonsei University College of Medicine, Seoul, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Myung-hee","family":"Yi","sequence":"additional","affiliation":[{"name":"Department of Tropical Medicine, Institute of Tropical Medicine, Arthropods of Medical Importance Resource Bank, Yonsei University College of Medicine, Seoul, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Myungjun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Tropical Medicine, Institute of Tropical Medicine, Arthropods of Medical Importance Resource Bank, Yonsei University College of Medicine, Seoul, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongjun","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Tropical Medicine, Institute of Tropical Medicine, Arthropods of Medical Importance Resource Bank, Yonsei University College of Medicine, Seoul, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soo Lim","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Tropical Medicine, Institute of Tropical Medicine, Arthropods of Medical Importance Resource Bank, Yonsei University College of Medicine, Seoul, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fred","family":"Odua","sequence":"additional","affiliation":[{"name":"Department of Global Health and Disease Control, Graduate School of Public Health, Yonsei University, Seoul, Republic of Korea"},{"name":"Department of Production, Nakasongola District Local Government, Uganda"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isuru","family":"Liyanagama","sequence":"additional","affiliation":[{"name":"Department of Global Health and Disease Control, Graduate School of Public Health, Yonsei University, Seoul, Republic of Korea"},{"name":"Department of Animal Production and Health, Southern Province, Sri Lanka"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2456-6298","authenticated-orcid":false,"given":"Ju Yeong","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Tropical Medicine, Institute of Tropical Medicine, Arthropods of Medical Importance Resource Bank, Yonsei University College of Medicine, Seoul, Republic of Korea"},{"name":"Department of Tropical Medicine, Graduate School of Medical Science, Brain Korea 21 Project, Yonsei University College of Medicine, Seoul, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2025,7,4]]},"reference":[{"key":"e_1_3_3_2_2","unstructured":"World Health Organization. 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