{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,4]],"date-time":"2026-11-04T12:35:24Z","timestamp":1793795724858,"version":"4.6.1"},"reference-count":44,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100005089","name":"Natural Science Foundation of Beijing Municipality","doi-asserted-by":"publisher","award":["7202045"],"award-info":[{"award-number":["7202045"]}],"id":[{"id":"10.13039\/501100005089","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82071448"],"award-info":[{"award-number":["82071448"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Science Progress"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Epileptogenic tubers (ETs) contribute to the epileptogenesis in patients with tuberous sclerosis complex (TSC). High mobility group protein B-1 (HMGB1) triggers inflammatory responses that impact neuronal electrical activity. We aimed to study the role of HMGB1 in epileptogenicity in TSC-related epilepsy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This study included both human and animal models. ETs were identified through comprehensive preoperative evaluations and postoperative seizure outcomes following tuberectomy. Immunofluorescence was performed to assess the positive area and fluorescence intensity of HMGB1 and IL-1\u03b2 in ETs and non-ETs. TSC2-GFAP-CKO mice and epileptic models were established. Mice in the prevention group (postnatal day 14) received daily injections of monoclonal HMGB1 or IL-1\u03b2 antibodies for 22 days. Mice in the intervention group (postnatal day 28) received identical treatments. Genetic and proteomic analyses of HMGB1 and IL-1\u03b2 were conducted on both TSC patients and animal models.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>HMGB1 and IL-1\u03b2 were significantly overexpressed in ETs compared to non-ETs. HMGB1 was predominantly nuclear in non-ETs but mainly cytoplasmic in ETs. In animal models, HMGB1 and IL-1\u03b2 expression at both the gene and protein levels was significantly upregulated in the TSC2-deficient group compared to the wild-type group. IL-1\u03b2 expression was lower in the HMGB1-intervention group than in the model control group. Seizure frequency was reduced in both HMGB1 and IL-1\u03b2 prevention and intervention groups compared to the model control.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The interference of HMGB1 and IL-1\u03b2 significantly reduced the frequency of seizure attacks in TSC. Epileptic seizures serve as key triggers for HMGB1 translocation and subsequent release into the cytoplasm, contributing to seizure recurrence.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/00368504251338653","type":"journal-article","created":{"date-parts":[[2025,6,12]],"date-time":"2025-06-12T03:45:47Z","timestamp":1749699947000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["HMGB1 mediates inflammation response of epileptogenicity in tuberous sclerosis complex-related epilepsy"],"prefix":"10.1177","volume":"108","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-1945-3450","authenticated-orcid":false,"given":"Suhui","family":"Kuang","sequence":"first","affiliation":[{"name":"Beijing Children's Hospital"},{"name":"Key Laboratory of Major Diseases in Children, Ministry of Education, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0156-7490","authenticated-orcid":false,"given":"Tinghong","family":"Liu","sequence":"additional","affiliation":[{"name":"Beijing Children's Hospital"},{"name":"Key Laboratory of Major Diseases in Children, Ministry of Education, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-3932-5610","authenticated-orcid":false,"given":"Liu","family":"Yuan","sequence":"additional","affiliation":[{"name":"Beijing Children's Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-5078-8301","authenticated-orcid":false,"given":"Jinshan","family":"Xu","sequence":"additional","affiliation":[{"name":"Beijing Children's Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4172-6081","authenticated-orcid":false,"given":"Yangshuo","family":"Wang","sequence":"additional","affiliation":[{"name":"Beijing Children's Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7410-0926","authenticated-orcid":false,"given":"Feng","family":"Chen","sequence":"additional","affiliation":[{"name":"Beijing Children's Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7292-745X","authenticated-orcid":false,"given":"Shuli","family":"Liang","sequence":"additional","affiliation":[{"name":"Beijing Children's Hospital"},{"name":"Key Laboratory of Major Diseases in Children, Ministry of Education, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2025,6,12]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(22)00213-7"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.3389\/fneur.2019.01093"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pediatrneurol.2019.12.016"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1528-1167.2007.01117.x"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00415-017-8507-y"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awz411"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11060-014-1429-y"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.0013-9580.2005.31704.x"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/nan.12444"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2016-3534"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1684\/epd.2020.1155"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-017-0799-4"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10072-023-06720-0"},{"key":"e_1_3_3_15_2","first-page":"5125","article-title":"HMGB1 Mediates microglia activation via the TLR4\/NF-\u03baB pathway in coriaria lactone induced epilepsy","volume":"17","author":"Shi Y","year":"2018","unstructured":"Shi Y, Zhang L, Teng J, et al. HMGB1 Mediates microglia activation via the TLR4\/NF-\u03baB pathway in coriaria lactone induced epilepsy. Mol Med Rep 2018; 17: 5125\u20135131.","journal-title":"Mol Med Rep"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/jnc.14663"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1080\/00207454.2019.1688809"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12868-019-0509-5"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41401-023-01087-6"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pediatrneurol.2021.07.011"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.3389\/fnhum.2021.680295"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/epi.16495"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/bph.15193"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.110.598334"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.108.769331"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1097\/SHK.0000000000000625"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-018-1712-3"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2022.147881"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchemneu.2020.101749"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.brs.2016.07.011"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1177\/08830738040190091401"},{"key":"e_1_3_3_32_2","first-page":"963","article-title":"Guide for the care and use of laboratory animals","volume":"327","author":"Care NRCCftUotGft and Animals UoL","year":"2011","unstructured":"Care NRCCftUotGft and Animals UoL. Guide for the care and use of laboratory animals. Publication 2011; 327: 963\u2013965.","journal-title":"Publication"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejpn.2018.05.006"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.3389\/fneur.2022.868633"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-019-02061-5"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2020.580357"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12031-016-0750-7"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2015.04.016"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-021-02265-1"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddq491"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.3389\/fnana.2020.00039"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2019.01.090"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-020-01973-4"},{"key":"e_1_3_3_44_2","first-page":"23","article-title":"N-acetyldopamine dimer inhibits neuroinflammation through the TLR4\/NF-&kappa;B and NLRP3\/caspase-1 pathways","volume":"55","author":"Huang L","year":"2022","unstructured":"Huang L, Gong L, Huo X, et al. N-acetyldopamine dimer inhibits neuroinflammation through the TLR4\/NF-&kappa;B and NLRP3\/caspase-1 pathways. Acta Biochim Biophys Sin 2022; 55: 23\u201333.","journal-title":"Acta Biochim Biophys Sin"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.7150\/thno.81406"}],"container-title":["Science Progress"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/00368504251338653","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/00368504251338653","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/00368504251338653","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T11:15:53Z","timestamp":1777634153000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/00368504251338653"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4]]},"references-count":44,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2025,4]]}},"alternative-id":["10.1177\/00368504251338653"],"URL":"https:\/\/doi.org\/10.1177\/00368504251338653","relation":{},"ISSN":["0036-8504","2047-7163"],"issn-type":[{"value":"0036-8504","type":"print"},{"value":"2047-7163","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4]]},"article-number":"00368504251338653"}}