{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T05:23:11Z","timestamp":1792819391095,"version":"4.5.0"},"reference-count":36,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Naresuan University (NU) and the National Science, Research, and Innovation Fund","award":["R2566B083"],"award-info":[{"award-number":["R2566B083"]}]},{"name":"Naresuan University (NU) and the National Science, Research, and Innovation Fund","award":["R2568B083"],"award-info":[{"award-number":["R2568B083"]}]},{"name":"Naresuan University (NU) and the National Science, Research, and Innovation Fund","award":["R2568B087"],"award-info":[{"award-number":["R2568B087"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Science Progress"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Cholangiocarcinoma is a lethal malignancy with an increasing incidence and mortality worldwide. Numerous studies have indicated that high-mobility group box 1 is associated with cancer progression. The modulation of subcellular high-mobility group box 1 expression is a major cause of chemotherapeutic drug resistance. This study aimed to demonstrate the role of high-mobility group box 1 in cholangiocarcinoma, including proving the concept of high-mobility group box 1 expression in patients with cholangiocarcinoma, cancer cell growth, migration, invasion, and the modulation of chemotherapeutic drug sensitivity in cholangiocarcinoma cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Twenty paraffin-embedded tissue samples were analyzed for high-mobility group box 1 expression using immunohistochemistry. High-mobility group box 1 expression was silenced in KKU-213AL5 cells through siRNA transfection, followed by in vitro assays to assess cell proliferation, migration, invasion, and drug sensitivity. Furthermore, the role of high-mobility group box 1 in regulating growth and metastasis-related signaling pathways was investigated using immunoblotting and protein\u2013protein interaction analysis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The results showed that high-mobility group box 1 was highly expressed in cholangiocarcinoma tissues compared to adjacent tissues (\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      -value\u2009&lt;\u20090.001), as well as the\n                      <jats:italic toggle=\"yes\">HMGB1<\/jats:italic>\n                      expression analysis from the TCGA database. Silencing high-mobility group box 1 by siRNA transfection resulted in the reduction of cholangiocarcinoma cell growth, migration, and invasion. Interestingly, high-mobility group box 1 silencing enhanced sensitivity to Gemcitabine and Cisplatin by increasing cell cytotoxicity compared to transfection control (\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      -value\u2009=\u20090.0002 and 0.0258, respectively). We further demonstrated that reduction of high-mobility group box 1 expression attenuates the essential signaling proteins, including Akt, Erk, and cyclin D1, which are crucial in cancer cell growth and metastasis signaling pathways.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This study demonstrates that high-mobility group box 1 plays a critical role in cholangiocarcinoma proliferation, migration and invasion, and may serve as a diagnostic biomarker. Targeting high-mobility group box 1 could enhance therapeutic outcomes, particularly in overcoming drug resistance.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/00368504251362343","type":"journal-article","created":{"date-parts":[[2025,7,21]],"date-time":"2025-07-21T10:45:42Z","timestamp":1753094742000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Higher expression of high-mobility group box 1 in cholangiocarcinoma and association with cell growth, in vitro migration and invasion, and chemo-drug sensitivity"],"prefix":"10.1177","volume":"108","author":[{"given":"Arunya","family":"Jiraviriyakul","sequence":"first","affiliation":[{"name":"Naresuan University"},{"name":"Naresuan University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samitanan","family":"Promchai","sequence":"additional","affiliation":[{"name":"Naresuan University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chatchai","family":"Nensat","sequence":"additional","affiliation":[{"name":"Naresuan University"},{"name":"Naresuan University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suphakit","family":"Khutanthong","sequence":"additional","affiliation":[{"name":"Naresuan University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nun","family":"Singpan","sequence":"additional","affiliation":[{"name":"Naresuan University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7799-2947","authenticated-orcid":false,"given":"Worawat","family":"Songjang","sequence":"additional","affiliation":[{"name":"Naresuan University"},{"name":"Naresuan University"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2025,7,21]]},"reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.gastha.2022.04.007"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.20517\/2394-5079.2021.130"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.aohep.2022.100737"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.5009\/gnl210346"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2022.850732"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12672-021-00446-1"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2020.110566"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41388-018-0485-8"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2021.10137"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2023.5272"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2021.739145"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.3724\/abbs.2022023"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.70363"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v11.i22.3392"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.3727\/096504017X15009778205068"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2024.1384109"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v21.i11.3256"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms231810540"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.2147\/JHC.S394074"},{"key":"e_1_3_4_21_2","first-page":"4431","article-title":"Functional role of SIRT1-induced HMGB1 expression and acetylation in migration, invasion and angiogenesis of ovarian cancer","volume":"22","author":"Jiang W","year":"2018","unstructured":"Jiang W, Jiang P, Yang R, et al. 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