{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T13:40:41Z","timestamp":1777902041102,"version":"3.51.4"},"reference-count":39,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Nanjing Medical Science and Technology Development Fund","award":["NO. YKK22239"],"award-info":[{"award-number":["NO. YKK22239"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Science Progress"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Sepsis, a systemic inflammatory response triggered by infection, is characterized by organ dysfunction. NETosis, a form of cell death involving the release of neutrophil extracellular traps (NETs), plays a crucial antimicrobial role during sepsis. This study aimed to explore the relationship between NETosis-related genes (NRGs) and 28-day mortality in patients with sepsis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This retrospective observational study utilized the Medical Information Mart for Intensive Care IV (MIMIC-IV) database. Univariate and multivariate logistic regression analyses were conducted to identify independent prognostic factors. A nomogram was then constructed to assess the potential of these factors in predicting 28-day mortality in patients with sepsis. Additionally, a Mendelian randomization (MR) study was performed to identify NRGs with causal associations to 28-day mortality in sepsis. Expression validation was carried out using the GSE65682 dataset from a public database, followed by identification of key genes. Enrichment analysis was performed to uncover the molecular mechanisms associated with these key genes in sepsis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A total of 909 patients with sepsis (706 survivors and 203 non-survivors) were identified from the MIMIC-IV database. Seven independent prognostic factors, including absolute neutrophil counts, were identified. The nomogram developed proved to be a reliable tool for predicting 28-day mortality in patients with sepsis. The MR study identified 12 NRGs with a unidirectional causal relationship to 28-day mortality, with AKT1 and CXCR2 emerging as key genes. Both genes are predominantly involved in immune-related pathways.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Analysis of the MIMIC-IV database highlighted neutrophil_abs as a significant prognostic factor for 28-day mortality in sepsis. Transcriptomic analysis identified AKT1 and CXCR2 as critical genes associated with 28-day mortality, providing insights into potential therapeutic strategies for sepsis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/00368504251374929","type":"journal-article","created":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T12:31:17Z","timestamp":1756729877000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Associations between NETosis-related genes and 28-day mortality in patients with sepsis: An observational retrospective study and bioinformatic analysis"],"prefix":"10.1177","volume":"108","author":[{"given":"Liu","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Scientific Research and Education, Taikang Xianlin Drum Tower Hospital, Affiliated Hospital of Medical College of Nanjing University, Nanjing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3147-5688","authenticated-orcid":false,"given":"Liang","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Taikang Xianlin Drum Tower Hospital, Affiliated Hospital of Medical College of Nanjing University, Nanjing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2025,9]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00134-024-07694-z"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S2213-2600(23)00468-X"},{"key":"e_1_3_3_4_2","first-page":"35","article-title":"Neutrophil extracellular traps in homeostasis and disease","volume":"9","author":"Wang H","year":"2024","unstructured":"Wang H, Kim SJ, Lei Y, et al. 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