{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T13:41:06Z","timestamp":1777902066066,"version":"3.51.4"},"reference-count":51,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Mahidol University (Fundamental Fund by National Science Research and Innovation Fund","award":["Fiscal year 2024"],"award-info":[{"award-number":["Fiscal year 2024"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Science Progress"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      The study investigated the role of the quorum sensing (QS) regulator BpsR4 in the physiology and virulence of\n                      <jats:italic toggle=\"yes\">Burkholderia pseudomallei<\/jats:italic>\n                      strain K96243.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      A\n                      <jats:italic toggle=\"yes\">B. pseudomallei bpsR4<\/jats:italic>\n                      knockout mutant (\u0394\n                      <jats:italic toggle=\"yes\">bpsR4<\/jats:italic>\n                      ) was constructed, and its gene expression profile was compared with that of the wild-type (WT) K96243 strain using RNA sequencing.\n                      <jats:italic toggle=\"yes\">In vitro<\/jats:italic>\n                      assays were conducted to assess growth rates, stress responses, antibiotic sensitivity, motility, and biofilm formation.\n                      <jats:italic toggle=\"yes\">Ex vivo<\/jats:italic>\n                      pathogenicity was evaluated using a human skin fibroblast infection model, and\n                      <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                      virulence was assessed in the\n                      <jats:italic toggle=\"yes\">Galleria mellonella<\/jats:italic>\n                      model.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">bpsR4<\/jats:italic>\n                      deletion led to significant transcriptional reprogramming, including the downregulation of genes involved in iron acquisition, sulfur metabolism, oxidative stress response, and redox homeostasis and upregulation of genes linked to motility, chemotaxis, and membrane transport. \u0394\n                      <jats:italic toggle=\"yes\">bpsR4<\/jats:italic>\n                      exhibited reduced tolerance to oxidative and heat stress and impaired biofilm formation but no significant change in motility. Additionally, \u0394\n                      <jats:italic toggle=\"yes\">bpsR4<\/jats:italic>\n                      displayed decreased susceptibility to meropenem. In both\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      and invertebrate infection models, the mutant demonstrated lower virulence than the WT strain.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      This study highlighted the involvement of BpsR4 in stress response, antimicrobial susceptibility, and virulence in\n                      <jats:italic toggle=\"yes\">B. pseudomallei<\/jats:italic>\n                      . Targeting QS pathways, particularly BpsR4 signaling, might represent a promising strategy to develop anti-virulence therapies that enhance antibiotic efficacy and improve clinical outcomes in melioidosis.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/00368504251394544","type":"journal-article","created":{"date-parts":[[2025,11,28]],"date-time":"2025-11-28T15:53:11Z","timestamp":1764345191000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Transcriptomic profile of BpsR4 and its roles in stress response, antibiotic susceptibility, biofilm formation, and pathogenesis in\n                    <i>Burkholderia pseudomallei<\/i>"],"prefix":"10.1177","volume":"108","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7388-7760","authenticated-orcid":false,"given":"Amporn","family":"Rungruengkitkun","sequence":"first","affiliation":[{"name":"Mahidol 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