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This study aimed to chemically and biologically characterize the parent methanolic extract and its derived fractions, obtained through sequential liquid\u2013liquid partitioning, from the sea cucumber\n                      <jats:italic toggle=\"yes\">Isostichopus<\/jats:italic>\n                      sp.\n                      <jats:italic toggle=\"yes\">aff<\/jats:italic>\n                      .\n                      <jats:italic toggle=\"yes\">badionotus<\/jats:italic>\n                      collected from the Colombian Caribbean Sea.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The extract and fractions were analyzed using ultra-high-performance liquid chromatography coupled with mass spectrometry and the Global Natural Products Social-based molecular networking platform, enabling dereplication and identification of metabolites. Additionally, the extract and fractions were evaluated against eight Gram-positive and Gram-negative bacterial strains using broth microdilution assays.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The dichloromethane fractions exhibited the highest antibacterial activity against both Gram-positive and Gram-negative bacteria. For the first time, chemical characterization allowed the identification of a series of metabolites present in the extract and fractions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      This study provides the first report on antibacterial activity in\n                      <jats:italic toggle=\"yes\">I.<\/jats:italic>\n                      sp.\n                      <jats:italic toggle=\"yes\">aff<\/jats:italic>\n                      .\n                      <jats:italic toggle=\"yes\">badionotus<\/jats:italic>\n                      , demonstrating that medium-polarity compounds in the dichloromethane fraction exhibited moderate bacterial inhibition, particularly against Gram-positive strains.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/00368504251407127","type":"journal-article","created":{"date-parts":[[2025,12,23]],"date-time":"2025-12-23T15:20:21Z","timestamp":1766503221000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Metabolite profiling-based characterization and antibacterial activity of sea cucumber\n                    <i>Isostichopus<\/i>\n                    sp.\n                    <i>aff. badionotus<\/i>\n                    from the Colombian Caribbean Sea"],"prefix":"10.1177","volume":"108","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9117-0405","authenticated-orcid":false,"given":"Manuel E","family":"Taborda-Mart\u00ednez","sequence":"first","affiliation":[{"name":"Bioprospecting Laboratory - 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