{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T05:58:28Z","timestamp":1790747908640,"version":"4.1.0"},"reference-count":19,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Endolife france","award":["20-24-501"],"award-info":[{"award-number":["20-24-501"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Science Progress"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Endometriosis is a systemic chronic disease with serious effects on various aspects of female health and wellbeing that has been associated with a significant increase in free circulating DNA (CfDNA) and gene methylation profile changes. Endometriosis is considered a worldwide health problem, affecting approximately 10\u201312% of women of reproductive age. It is defined as an estrogen-dependent syndrome associated with severe pain and a distinct epigenetic signature. The purpose of this study was to evaluate the effect of deoxyribonuclease (DNase) I therapy on these anomalies.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This study is a proof-of-concept investigation presenting preliminary data from sixteen patients with endometriosis who were evaluated during infertility consultations. CfDNA extraction, quantification and methylation status tests were performed using commercial kits. DNase I was administered subcutaneously every 2 days for one month, and the pre-treatment and post-treatment CfDNA levels and methylation status, along with the degree of patient discomfort, were compared.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>DNase I therapy was associated with a decrease in the overall CfDNA level. Targeted methylation profiles showed exploratory changes in several genes, presented descriptively because no paired inferential reanalysis was performed. Moreover, most patients (15\/16) reported a reduction in pain, an improvement in their ability to assume professional activity and fewer sexual function difficulties.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This proof-of-concept study suggests that DNase I therapy may be associated with decreased CfDNA levels and changes in methylation profiles. Patient-reported improvements in pain and quality of life were observed, but they cannot be causally attributed to treatment in the absence of a placebo-controlled design. Further research into the potential use of DNase-based medicine for endometriosis patients involving more participants is warranted.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/00368504261479982","type":"journal-article","created":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T17:22:55Z","timestamp":1786728175000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Effect of deoxyribonuclease I therapy on free circulating DNA level and gene methylation profile changes in patients with endometriosis: Proof of concept and preliminary data"],"prefix":"10.1177","volume":"109","author":[{"given":"Moncef","family":"Benkhalifa","sequence":"first","affiliation":[{"name":"Picardie University Jules Verne"},{"name":"PERITOX-(UMR-I 01)"},{"name":"UPJV\/INERIS"},{"name":"UPJV"},{"name":"CURS"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Piquemal","sequence":"additional","affiliation":[{"name":"ACOBIOM"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-9685-7633","authenticated-orcid":false,"given":"Marwa","family":"Lahimer","sequence":"additional","affiliation":[{"name":"Picardie University Jules Verne"},{"name":"PERITOX-(UMR-I 01)"},{"name":"UPJV\/INERIS"},{"name":"UPJV"},{"name":"CURS"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chafika","family":"Nadifi","sequence":"additional","affiliation":[{"name":"Mohammed VI University of Health and Sciences (UM6SS)"},{"name":"Hassan II University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre Alain","family":"Menoud","sequence":"additional","affiliation":[{"name":"UNILABS"},{"name":"Molecular Diagnostic Laboratory"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed","family":"Zarqaoui","sequence":"additional","affiliation":[{"name":"IRIFIV. Les IRIS Clinic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosalie","family":"Cabry","sequence":"additional","affiliation":[{"name":"Picardie University Jules Verne"},{"name":"PERITOX-(UMR-I 01)"},{"name":"UPJV\/INERIS"},{"name":"UPJV"},{"name":"CURS"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatima Azzahra","family":"Lahlou","sequence":"additional","affiliation":[{"name":"Mohammed VI University of Health and Sciences (UM6SS)"},{"name":"Hassan II University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Tesarik","sequence":"additional","affiliation":[{"name":"MARGen Clinic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,8,14]]},"reference":[{"key":"e_1_3_6_2_2","doi-asserted-by":"publisher","DOI":"10.3390\/biom15010069"},{"key":"e_1_3_6_3_2","doi-asserted-by":"publisher","DOI":"10.1080\/0167482X.2019.1659775"},{"key":"e_1_3_6_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/AJP.0000000000000539"},{"key":"e_1_3_6_5_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph17134683"},{"key":"e_1_3_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.xfnr.2024.01.003"},{"key":"e_1_3_6_7_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms26062458"},{"key":"e_1_3_6_8_2","doi-asserted-by":"publisher","DOI":"10.3390\/biom12081055"},{"key":"e_1_3_6_9_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms26093979"},{"key":"e_1_3_6_10_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12888-023-04823-8"},{"key":"e_1_3_6_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jri.2025.104521"},{"key":"e_1_3_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1472-6483(10)60011-9"},{"key":"e_1_3_6_13_2","first-page":"2025121221","article-title":"Effect of Deoxyribonuclease I Therapy on Free Circulating DNA Level and Gene Methylation Profile Changes in Patients with Endometriosis: Preliminary Data","volume":"15","author":"Benkhalifa M","year":"2025","unstructured":"Benkhalifa M, Piquemal D, Lahimer M, et al. Effect of Deoxyribonuclease I Therapy on Free Circulating DNA Level and Gene Methylation Profile Changes in Patients with Endometriosis: Preliminary Data. Preprints 2025; 15: 2025121221, Preprint posted online December. https:\/\/doi.org\/10.20944\/preprints202512.1221.v1","journal-title":"Preprints"},{"key":"e_1_3_6_14_2","unstructured":"CONSORT 2025 statement: updated guideline for reporting randomised trials. The BMJ. Accessed April 21 2026.https:\/\/www.bmj.com\/content\/389\/bmj-2024-081123"},{"key":"e_1_3_6_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2021.04.041"},{"issue":"7","key":"e_1_3_6_16_2","first-page":"827","article-title":"Endometriosis: Molecular Pathophysiology and Recent Treatment Strategies-Comprehensive Literature Review","volume":"17","author":"Sad\u0142ocha M","year":"2024","unstructured":"Sad\u0142ocha M, Toczek J, Major K, et al. Endometriosis: Molecular Pathophysiology and Recent Treatment Strategies-Comprehensive Literature Review. Pharm Basel Switz 2024; 17(7): 827. https:\/\/doi.org\/10.3390\/ph17070827","journal-title":"Pharm Basel Switz"},{"key":"e_1_3_6_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-024-02082-x"},{"key":"e_1_3_6_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2024.149102"},{"key":"e_1_3_6_19_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2024.1425212"},{"key":"e_1_3_6_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/s44318-024-00253-8"}],"container-title":["Science Progress"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/00368504261479982","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/00368504261479982","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/00368504261479982","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T05:17:48Z","timestamp":1790745468000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/00368504261479982"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7]]},"references-count":19,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,7]]}},"alternative-id":["10.1177\/00368504261479982"],"URL":"https:\/\/doi.org\/10.1177\/00368504261479982","relation":{},"ISSN":["0036-8504","2047-7163"],"issn-type":[{"value":"0036-8504","type":"print"},{"value":"2047-7163","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7]]},"article-number":"00368504261479982"}}