{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T18:53:33Z","timestamp":1782586413747,"version":"3.54.5"},"reference-count":15,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2019,12,5]],"date-time":"2019-12-05T00:00:00Z","timestamp":1575504000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Trop Doct"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:p>The present study examined hospital-based serological tests of rickettsial infections and assessment for diagnosis of pyrexia of unknown origin (PUO). Blood samples were tested for Weil Felix antigens, ELISA for scrub typhus group and polymerase chain reaction (PCR) to detect the presence of DNA of spotted and scrub typhus group with the help of specific oligonucleotide. We tested 450 patient samples and found 101 Weil Felix-positive with 15 having \u2265320 titres. IgM ELISA identified 32 (7.1%) positive cases. Positive PCR was seen in 13 (2.9%) samples, being only 40.1% of those testing positive for ELISA. Rickettsial infection is predominantly diagnosed through serological evidence in combination with molecular techniques. The Weil Felix test has a number of disadvantages and tends to provide false-positive results in a number of scenarios, especially where scrub typhus and spotted fever are widely distributed.<\/jats:p>","DOI":"10.1177\/0049475519892086","type":"journal-article","created":{"date-parts":[[2019,12,6]],"date-time":"2019-12-06T01:57:59Z","timestamp":1575597479000},"page":"122-124","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["Hospital based serological evidence of rickettsial diseases and assessment diagnostic tests of pyrexia of unknown origin"],"prefix":"10.1177","volume":"50","author":[{"family":"Muzaheed","sequence":"first","affiliation":[{"name":"Assistant Professor, Department of Clinical Laboratory Science, College of Applied Medical Sciences, Imam Abdulrahman Bin Faisal University, Dammam, Kingdom of Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amal","family":"J Fatani","sequence":"additional","affiliation":[{"name":"Professor, Department of Pharmacology & Toxicology, College of Pharmacy, King Saud University, Riyadh, Kingdom of Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6322-8059","authenticated-orcid":false,"given":"Darshan","family":"D Divakar","sequence":"additional","affiliation":[{"name":"Researcher, Dental Biomaterials Research Chair, Dental Health Department, College of Applied Medical Sciences, King Saud University, Riyadh, Kingdom of Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanjay","family":"Rathod","sequence":"additional","affiliation":[{"name":"Lecturer, Department of Post Graduate Studies and Research in Microbiology, Gulbarga University, Gulbarga, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7891-4011","authenticated-orcid":false,"given":"Mustafa","family":"S Aloahd","sequence":"additional","affiliation":[{"name":"Visiting Scholar, College of Life Science, Maulana Azad College of Arts and Science, Aurangabad, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2019,12,5]]},"reference":[{"key":"bibr1-0049475519892086","doi-asserted-by":"publisher","DOI":"10.1007\/s12038-008-0073-0"},{"key":"bibr2-0049475519892086","doi-asserted-by":"publisher","DOI":"10.1093\/med\/9780199671328.001.0001"},{"key":"bibr3-0049475519892086","volume-title":"A synopsis of infectious and tropical diseases","author":"Woodruff AW","year":"2013"},{"key":"bibr4-0049475519892086","doi-asserted-by":"publisher","DOI":"10.1097\/MAJ.0b013e31824ae504"},{"key":"bibr5-0049475519892086","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-540-78598-9"},{"key":"bibr6-0049475519892086","doi-asserted-by":"publisher","DOI":"10.18203\/2349-3933.ijam20172033"},{"key":"bibr7-0049475519892086","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.11.5094-5098.2003"},{"key":"bibr8-0049475519892086","doi-asserted-by":"publisher","DOI":"10.1086\/339908"},{"key":"bibr9-0049475519892086","doi-asserted-by":"publisher","DOI":"10.1128\/JB.173.5.1576-1589.1991"},{"key":"bibr10-0049475519892086","first-page":"128","volume":"126","author":"Kamarasu K","year":"2007","journal-title":"Indian J Med Res"},{"key":"bibr11-0049475519892086","doi-asserted-by":"crossref","unstructured":"Jeong YJ, Kim S, Wook YD, Lee JW, Kim KI, Lee SH. Scrub typhus: clinical, pathologic, and imaging findings.\n                      Radiographics\n                      2007; 27: 161\u201372.","DOI":"10.1148\/rg.271065074"},{"key":"bibr12-0049475519892086","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.8.2271-2278.1998"},{"key":"bibr13-0049475519892086","first-page":"101","volume":"126","author":"Batra HV","year":"2007","journal-title":"Indian J Med Res"},{"key":"bibr14-0049475519892086","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.11.6628-6636.2004"},{"key":"bibr15-0049475519892086","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(02)02445-9"}],"container-title":["Tropical Doctor"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0049475519892086","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/0049475519892086","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0049475519892086","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T13:32:00Z","timestamp":1777642320000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/0049475519892086"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,12,5]]},"references-count":15,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2020,4]]}},"alternative-id":["10.1177\/0049475519892086"],"URL":"https:\/\/doi.org\/10.1177\/0049475519892086","relation":{},"ISSN":["0049-4755","1758-1133"],"issn-type":[{"value":"0049-4755","type":"print"},{"value":"1758-1133","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,12,5]]}}}