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The primary aim of our prospective diagnostic study was to compare available laboratory methods for the diagnosis of Entamoeba histolytica in clinical samples with loop-mediated isothermal amplification. We compared deoxyribonucleic acid (DNA) analysis methods, namely, loop-mediated isothermal amplification and reverse transcriptase polymerase chain reaction and enzyme-linked immunosorbent assay (ELISA) using pus, stool and blood samples from 200 patients with clinical and radiological diagnosis of amoebic liver abscess. Loop-mediated isothermal amplification had significantly higher sensitivity (88%) as compared to reverse transcriptase polymerase chain reaction (64%) and excellent specificity (100%).<\/jats:p>","DOI":"10.1177\/00494755211018050","type":"journal-article","created":{"date-parts":[[2021,6,10]],"date-time":"2021-06-10T02:21:11Z","timestamp":1623291671000},"page":"488-491","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["Utility of loop-mediated isothermal amplification as a point-of-care test in diagnosis of amoebic liver abscess"],"prefix":"10.1177","volume":"51","author":[{"given":"Dipti","family":"Handa","sequence":"first","affiliation":[{"name":"Ex-postgraduate student, Department of General Medicine, Government Medical College and Hospital, Chandigarh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3992-1013","authenticated-orcid":false,"given":"Monica","family":"Gupta","sequence":"additional","affiliation":[{"name":"Professor, Department of General Medicine, Government Medical College and Hospital, Chandigarh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarabmeet Singh","family":"Lehl","sequence":"additional","affiliation":[{"name":"Professor, Department of General Medicine, Government Medical College and Hospital, Chandigarh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amit","family":"Gupta","sequence":"additional","affiliation":[{"name":"Associate Consultant, Department of Orthopaedics, Department of Orthopaedics, Deep Hospital, Ludhiana, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ram","family":"Singh","sequence":"additional","affiliation":[{"name":"Professor, Department of General Medicine, Government Medical College and Hospital, Chandigarh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2021,6,9]]},"reference":[{"key":"bibr1-00494755211018050","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2019.104018"},{"key":"bibr2-00494755211018050","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.01394"},{"key":"bibr3-00494755211018050","doi-asserted-by":"publisher","DOI":"10.1186\/s41182-020-0193-2"},{"key":"bibr4-00494755211018050","first-page":"511","volume":"20","author":"Fotedar R","year":"2007","journal-title":"ClinMicrobiol Rev"},{"key":"bibr5-00494755211018050","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-018-3379-3"},{"key":"bibr6-00494755211018050","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.9.3235-3239.2000"},{"key":"bibr7-00494755211018050","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.16.4.713-729.2003"},{"key":"bibr8-00494755211018050","first-page":"4569","volume":"44","author":"Leo M","year":"2006","journal-title":"J ClinMicrobiol"},{"key":"bibr9-00494755211018050","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.9.4801-4806.2005"},{"key":"bibr10-00494755211018050","doi-asserted-by":"publisher","DOI":"10.1590\/S1413-86702003000200002"},{"key":"bibr11-00494755211018050","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-7-47"},{"key":"bibr12-00494755211018050","doi-asserted-by":"publisher","DOI":"10.4103\/2229-5070.175116"},{"key":"bibr13-00494755211018050","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00105-09"},{"key":"bibr14-00494755211018050","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.593"},{"key":"bibr15-00494755211018050","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.12.e63"},{"key":"bibr16-00494755211018050","doi-asserted-by":"crossref","first-page":"96","DOI":"10.21315\/mjms2018.25.1.11","volume":"25","author":"Keikha M","year":"2018","journal-title":"Malaysian J Med Sci"},{"key":"bibr17-00494755211018050","doi-asserted-by":"publisher","DOI":"10.1007\/s11274-012-1154-7"},{"key":"bibr18-00494755211018050","doi-asserted-by":"crossref","unstructured":"Voller A, Bartlett A, Bidwell DE. 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