{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T02:03:59Z","timestamp":1787018639775,"version":"build-2736575974"},"reference-count":20,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2024,2,4]],"date-time":"2024-02-04T00:00:00Z","timestamp":1707004800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Trop Doct"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:p>Permanent stains such as trichrome have better sensitivity but are time-consuming and the fixative includes toxic mercuric chloride. Thus, a newer modification was tested and found to be a superior, faster and safer staining technique for intestinal parasitic detection. Our study lasted 9 months and a single stool sample was collected from each enrolled patient. We evaluated classical trichrome (T1 \u2013 using Schaudinn fixative) with newer modifications, which involved different fixatives with mordant combinations (T2 \u2013 acetic acid\u2009+\u2009hydrated aluminium sulphate, T3 \u2013 citric acid\u2009+\u2009copper sulphate hydrate). Conventional PCR targeting Entamoeba histolytica, Giardia lamblia and Cryptosporidium spp. was taken as the reference. Out of 175 stool samples, 25.1% protozoa were identified by wet mount, 24% by each T1 and T2, 25.7% by T3. Statistically, T3 and T2 had higher sensitivity as compared to T1 and wet mount when PCR was used as reference.<\/jats:p>","DOI":"10.1177\/00494755241227466","type":"journal-article","created":{"date-parts":[[2024,2,5]],"date-time":"2024-02-05T02:54:30Z","timestamp":1707101670000},"page":"139-146","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["Modified trichrome stain for faster and improved detection of intestinal protozoan parasites"],"prefix":"10.1177","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5608-8919","authenticated-orcid":false,"given":"Priya","family":"Datta","sequence":"first","affiliation":[{"name":"Assistant Professor, Department of Medical Parasitology, PGIMER, Chandigarh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Puja","family":"Garg","sequence":"additional","affiliation":[{"name":"PhD Scholar, Department of Medical Parasitology, PGIMER, 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