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Here, we describe a case of laryngeal blastomycosis that presented as slowly progressive dysphonia. While a preliminary diagnosis was made using routine histopathology, a species identification of Blastomyces dermatitidis was made using polymerase chain reaction amplification and rapid genotyping without the need for fungal culture. All symptoms resolved following 1 month of antifungal therapy. Rapid molecular differentiation of B dermatitidis from Blastomyces gilchristii provides important insights into pathogenesis given recent recognition of differences in clinical spectra.<\/jats:p>","DOI":"10.1177\/0145561321991342","type":"journal-article","created":{"date-parts":[[2021,3,8]],"date-time":"2021-03-08T13:24:52Z","timestamp":1615209892000},"page":"NP157-NP160","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["Direct Tissue PCR and Genotyping for Species Identification in a Case of Laryngeal Blastomycosis"],"prefix":"10.1177","volume":"102","author":[{"given":"Thomas R.","family":"Fritsche","sequence":"first","affiliation":[{"name":"Division of Laboratory Medicine, Marshfield Clinic Health System, Marshfield, WI, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3814-9655","authenticated-orcid":false,"given":"Jennifer L.","family":"Anderson","sequence":"additional","affiliation":[{"name":"Integrated Research and Development Laboratory, Marshfield Clinic Research Institute, Marshfield Clinic Health System, Marshfield, WI, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deepa","family":"Bassi","sequence":"additional","affiliation":[{"name":"Division of Laboratory Medicine, Marshfield Clinic Health System, Marshfield, WI, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew C.","family":"Hall","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Marshfield Clinic Health System, Marshfield, WI, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timothy R.","family":"Boyle","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, Marshfield Clinic Health System, Marshfield, WI, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer K.","family":"Meece","sequence":"additional","affiliation":[{"name":"Integrated Research and Development Laboratory, Marshfield Clinic Research Institute, Marshfield Clinic Health System, Marshfield, WI, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2021,3,8]]},"reference":[{"key":"bibr1-0145561321991342","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0059237"},{"key":"bibr2-0145561321991342","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciy483"},{"key":"bibr3-0145561321991342","doi-asserted-by":"publisher","DOI":"10.1111\/myc.12601"},{"key":"bibr4-0145561321991342","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5520(02)00038-7"},{"issue":"9","key":"bibr5-0145561321991342","first-page":"416","volume":"3","author":"Gauthier G","year":"2008","journal-title":"Microbe Wash DC"},{"issue":"22","key":"bibr6-0145561321991342","first-page":"61","volume":"22","author":"Pappas PG","year":"2002","journal-title":"Curr Clin Top Infect Dis"},{"key":"bibr7-0145561321991342","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jis756"},{"key":"bibr8-0145561321991342","unstructured":"Woods GL, Gutierrez Y. 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