{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T15:36:49Z","timestamp":1760888209816,"version":"build-2065373602"},"reference-count":27,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2006,9,1]],"date-time":"2006-09-01T00:00:00Z","timestamp":1157068800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Parenter Enteral Nutr"],"published-print":{"date-parts":[[2006,9]]},"abstract":"<jats:p><jats:italic>Background:<\/jats:italic> Without enteral nutrition, the mass and function of gut\u2010associated lymphoid tissue (GALT), a center of systemic mucosal immunity, are reduced. Therefore, new therapeutic methods, designed to preserve mucosal immunity during parenteral nutrition (PN), are needed. Our recent study revealed that exogenous interleukin\u20107 (IL\u20107; 1 \u03bcg\/kg twice a day) restores the GALT cell mass lost during intravenous (IV) PN but does not improve secretory immunoglobulin A (IgA) levels. Herein, we studied the IL\u20107 dose response to determine the optimal IL\u20107 dose for recovery of GALT mass and function during IV PN. We hypothesized that a high dose of IL\u20107 would increase intestinal IgA levels, as well as GALT cell numbers. <jats:italic>Methods:<\/jats:italic> Male mice (n = 42) were randomized to chow, IL\u20107\u20100, IL\u20107\u20100.1, IL\u20107\u20100.33, IL\u20107\u20101 and IL\u20107\u20103.3 groups and underwent jugular vein catheter insertion. The IL\u20107 groups were fed a standard PN solution and received IV injections of normal saline (IL\u20107\u20100), 0.1, 0.33, 1, or 3.3 \u03bcg\/kg of IL\u20107 twice a day. The chow group was fed chow <jats:italic>ad libitum<\/jats:italic>. After 5 days of treatment, the entire small intestine was harvested and lymphocytes were isolated from Peyer's patches (PPs), intraepithelial (IE) spaces, and the lamina propria (LP). The lymphocytes were counted and phenotypes determined by flow cytometry (\u03b1\u03b2TCR, \u03b3\u03b4TCR, CD4, CD8, B cell). IgA levels of small intestinal washings were also examined using ELISA (enzyme\u2010linked immunoabsorbent assay). <jats:italic>Results:<\/jats:italic> IL\u20107 dose\u2010dependently increased total lymphocyte numbers in PPs and the LP. The number of lymphocytes harvested from IE spaces reached a plateau at 1 \u03bcg\/kg of IL\u20107. There were no significant differences in any phenotype percentages at any GALT sites among the groups. IgA levels of intestinal washings were significantly higher in the chow group than in any of the IL\u20107 groups, with similar levels in all IL\u20107 groups. <jats:italic>Conclusions:<\/jats:italic> Exogenous IL\u20107 dose\u2010dependently reverses PN\u2010induced GALT cell loss, with no major changes in small intestinal IgA levels. IL\u20107 treatment during PN appears to have beneficial effects on gut immunity, but other therapeutic methods are needed to restore secretory IgA levels.<\/jats:p>","DOI":"10.1177\/0148607106030005388","type":"journal-article","created":{"date-parts":[[2008,5,14]],"date-time":"2008-05-14T19:51:26Z","timestamp":1210794686000},"page":"388-394","source":"Crossref","is-referenced-by-count":10,"title":["Interleukin\u20107 Dose\u2010Dependently Restores Parenteral Nutrition\u2013Induced Gut\u2010Associated Lymphoid Tissue Cell Loss but Does Not Improve Intestinal Immunoglobulin A 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