{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T19:19:46Z","timestamp":1773947986198,"version":"3.50.1"},"reference-count":26,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,8,18]],"date-time":"2014-08-18T00:00:00Z","timestamp":1408320000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2014,8,18]],"date-time":"2014-08-18T00:00:00Z","timestamp":1408320000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Parenter Enteral Nutr"],"published-print":{"date-parts":[[2016,3]]},"abstract":"<jats:p><jats:italic>Background:<\/jats:italic> The purpose of this study was to assess the effect of arginine supplementation on arginase activity, tumor necrosis factor\u2013\u03b1 (TNF\u2010\u03b1) and interleukin\u201010 (IL\u201010) synthesis in cultured splenic macrophages from a murine model of intestinal obstruction (IO). The effects of nitric oxide synthase (iNOS) inhibition were also studied using iNOS <jats:italic>knockout<\/jats:italic> animals. <jats:italic>Material and Methods<\/jats:italic>: Male C57BL6\/J <jats:italic>wild\u2010type<\/jats:italic> (WT) and iNOS <jats:italic>knockout<\/jats:italic> (iNOS\u2013\/\u2013) mice were randomized into 6 groups: <jats:italic>Sham<\/jats:italic> and <jats:italic>Sham\u2013<\/jats:italic>\/\u2013 (standard chow), <jats:italic>IO and IO\u2013\/\u2013<\/jats:italic> (standard chow + IO), and <jats:italic>Arg and Arg\u2013\/\u2013<\/jats:italic> (standard chow supplemented with arginine + IO). After 7 days of treatment with standard or supplemented chow, IO was induced. Arginase activity as well as TNF\u2010\u03b1 and IL\u201010 levels were analyzed in splenic macrophage cultures. <jats:italic>Results<\/jats:italic>: Arginine supplementation and the absence of iNOS increased arginase activity in splenic macrophages (Arg, IO<jats:italic>\u2013\/\u2013<\/jats:italic>, and Arg\u2013\/\u2013 groups vs the <jats:italic>Sham<\/jats:italic> group; <jats:italic>P<\/jats:italic> &lt; .05). Arginine was also related to a decrease in TNF\u2010\u03b1 levels (Arg vs IO group, <jats:italic>P<\/jats:italic> &lt; .05) and maintenance of IL\u201010 levels (Arg vs other groups, <jats:italic>P<\/jats:italic> &gt; .05). The inhibition of iNOS did not result in effects on the concentration of cytokines (<jats:italic>Sham<\/jats:italic>\u2013\/\u2013, IO\u2013\/\u2013, and Arg\u2013\/\u2013 vs other, <jats:italic>P<\/jats:italic> &lt; .05). <jats:italic>Conclusions<\/jats:italic>: Arginine supplementation and iNOS inhibition led to increased arginase activity. Arginine availability decreased plasma TNF\u2010\u03b1 levels, which may be directly related to nitric oxide derived from arginine.<\/jats:p>","DOI":"10.1177\/0148607114546374","type":"journal-article","created":{"date-parts":[[2014,8,19]],"date-time":"2014-08-19T02:13:53Z","timestamp":1408414433000},"page":"417-422","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":12,"title":["Arginine Supplementation Induces Arginase Activity and Inhibits TNF\u2010\u03b1 Synthesis in Mice Spleen Macrophages After Intestinal Obstruction"],"prefix":"10.1002","volume":"40","author":[{"given":"Iara Eliza Pac\u00edfico","family":"Quirino","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matheus Batista Heitor","family":"Carneiro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valbert 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