{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T00:35:22Z","timestamp":1760920522515,"version":"build-2065373602"},"reference-count":34,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2014,10,14]],"date-time":"2014-10-14T00:00:00Z","timestamp":1413244800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2014,10,14]],"date-time":"2014-10-14T00:00:00Z","timestamp":1413244800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Parenter Enteral Nutr"],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:p><jats:italic>Background:<\/jats:italic> The aim of the present study was to elucidate whether the administration of antioxidant\u2010rich nutrients, including branched\u2010chain amino acids (BCAAs), microelements, and vitamins, both alone and in combination, has a positive impact on liver function in a nonalcoholic steatohepatitis (NASH) mouse model and identify the mechanisms underlying these effects. <jats:italic>Methods:<\/jats:italic> Seven\u2010week\u2010old male KKAy mice fed a methionine\u2010 and choline\u2010deficient diet (MCD) for 4 weeks were divided into 7 groups and fed the following planned diets for another 4 weeks: group A (normal diet), group B (MCD; control), group C (MCD with rich microelements), group D (MCD with rich BCAAs), group E (MCD with rich microelements and BCAAs), and group F (MCD with rich microelements, BCAAs, and vitamins). We then conducted biochemical assays, histological analyses, immunohistochemistry for 8\u2010hydroxy\u20102\u2032\u2010deoxyguanosine (8\u2010OHdG) and 4\u2010hydroxy\u20102\u2032\u2010nonenal (4\u2010HNE), and Western blotting for insulin glucose signaling, lipid metabolism, and endoplasmic reticulum (ER) stress\u2013related signaling in liver specimens obtained from mice in each group. <jats:italic>Results:<\/jats:italic> The morphometric grades of all NASH\u2010related findings and the mean degree of 8\u2010OHdG immunolocalization in groups D\u2013F were significantly lower than those observed in group B. The expression levels of insulin receptor \u03b2 subunit (IR\u03b2) and p\u2010elF in groups E and F and those of phosphatidyl\u2010inositol 3 kinase (PI3K85), p\u2010AcelCoA, and PERK in group F were similar to those noted in group A. <jats:italic>Conclusions:<\/jats:italic> The administration of a combination of antioxidant\u2010rich nutrients, including BCAAs and microelements, is likely to suppress the progression of NASH by reducing oxidative stress, primarily via the downregulation of the ER stress pathway.<\/jats:p>","DOI":"10.1177\/0148607114555160","type":"journal-article","created":{"date-parts":[[2014,10,14]],"date-time":"2014-10-14T22:00:52Z","timestamp":1413324052000},"page":"519-528","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":11,"title":["Branched\u2010Chain Amino Acid\u2013Rich Supplements Containing Microelements Have Antioxidant Effects on Nonalcoholic Steatohepatitis in Mice"],"prefix":"10.1002","volume":"40","author":[{"given":"Hiroaki","family":"Tanaka","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suguru","family":"Fukahori","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shinji","family":"Baba","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takato","family":"Ueno","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ramadoss","family":"Sivakumar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Minoru","family":"Yagi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kimio","family":"Asagiri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shinji","family":"Ishii","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshiaki","family":"Tanaka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,10,14]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1053\/jhep.2002.30692"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1053\/gast.2001.23256"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1053\/jhep.2002.30690"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2007.12.029"},{"issue":"1","key":"e_1_2_7_6_1","first-page":"S17","article-title":"Pathophysiology of nonalcoholic steatohepatitis","volume":"40","author":"McCullough AJ","year":"2006","journal-title":"J Clin Gastroenterol"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1467-789X.2004.00126.x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnutbio.2007.10.001"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.24001"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.2337\/diab.46.6.983"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(98)70599-2"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.52.1.1"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mam.2007.09.014"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2011.520"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0907929"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.2003.08699.x"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(98)70644-4"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2007.12.008"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.179.6.4180"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1478-3231.2007.01559.x"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1872-034X.2007.00081.x"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2036.2009.04122.x"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v16.i21.2638"},{"issue":"6","key":"e_1_2_7_24_1","first-page":"349","article-title":"Effects of zinc deficiency\/zinc supplementation on ammonia metabolism in patients with decompensated liver cirrhosis","volume":"55","author":"Yoshida Y","year":"2001","journal-title":"Acta Med Okayama"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(99)70506-8"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.20701"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hepres.2006.07.009"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10620-007-9900-7"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M800042-JLR200"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.51.1.7"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI0215593"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1103160"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200401157"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1271\/bbb.130130"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2003.09.020"}],"container-title":["Journal of Parenteral and Enteral Nutrition"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0148607114555160","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/0148607114555160","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/aspenjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1177\/0148607114555160","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T11:54:31Z","timestamp":1760874871000},"score":1,"resource":{"primary":{"URL":"https:\/\/aspenjournals.onlinelibrary.wiley.com\/doi\/10.1177\/0148607114555160"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,10,14]]},"references-count":34,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2016,5]]}},"alternative-id":["10.1177\/0148607114555160"],"URL":"https:\/\/doi.org\/10.1177\/0148607114555160","archive":["Portico"],"relation":{},"ISSN":["0148-6071","1941-2444"],"issn-type":[{"type":"print","value":"0148-6071"},{"type":"electronic","value":"1941-2444"}],"subject":[],"published":{"date-parts":[[2014,10,14]]}}}