{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:44:25Z","timestamp":1790815465601,"version":"4.1.0"},"reference-count":37,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2015,5,13]],"date-time":"2015-05-13T00:00:00Z","timestamp":1431475200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2015,5,13]],"date-time":"2015-05-13T00:00:00Z","timestamp":1431475200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Parenter Enteral Nutr"],"published-print":{"date-parts":[[2016,11]]},"abstract":"<jats:p><jats:italic>Background:<\/jats:italic>Recent studies showed that patients with diarrhea\u2010predominant irritable bowel syndrome (IBS\u2010D) had an increased intestinal permeability as well as a decreased expression of tight junctions. Glutamine, the major substrate of rapidly dividing cells, is able to modulate intestinal permeability and tight junction expression in other diseases. We aimed to evaluate, ex vivo, glutamine effects on tight junction proteins, claudin\u20101 and occludin, in the colonic mucosa of patients with IBS\u2010D. <jats:italic>Materials and Methods:<\/jats:italic> Twelve patients with IBS\u2010D, diagnosed with the Rome III criteria, were included (8 women\/4 men, aged 40.7 \u00b1 6.9 years). Colonic biopsy specimens were collected and immediately incubated for 18 hours in culture media with increasing concentrations of glutamine from 0.6\u201310 mmol\/L. Claudin\u20101 and occludin expression was then measured by immunoblot, and concentrations of cytokines were assessed by multiplex technology. Claudin\u20101 expression was affected by glutamine (<jats:italic>P<\/jats:italic> &lt; .05, analysis of variance). In particularly, 10 mmol\/L glutamine increased claudin\u20101 expression compared with 0.6 mmol\/L glutamine (0.47 \u00b1 0.04 vs 0.33 \u00b1 0.03, <jats:italic>P<\/jats:italic> &lt; .05). In contrast, occludin expression was not significantly modified by glutamine. Interestingly, glutamine effect was negatively correlated to claudin\u20101 (Pearson <jats:italic>r<\/jats:italic> = \u20100.83, <jats:italic>P<\/jats:italic> &lt; .001) or occludin basal expression (Pearson <jats:italic>r<\/jats:italic> = \u20100.84, <jats:italic>P<\/jats:italic> &lt; .001), suggesting that glutamine had more marked effects when tight junction protein expression was altered. Cytokine concentrations in culture media were not modified by glutamine treatment. <jats:italic>Conclusion:<\/jats:italic> Glutamine increased claudin\u20101 expression in the colonic mucosa of patients with IBS\u2010D. In addition, glutamine effect seems to be dependent on basal expression of tight junction proteins.<\/jats:p>","DOI":"10.1177\/0148607115587330","type":"journal-article","created":{"date-parts":[[2015,5,13]],"date-time":"2015-05-13T22:21:08Z","timestamp":1431555668000},"page":"1170-1176","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":40,"title":["Glutamine Restores Tight Junction Protein Claudin\u20101 Expression in Colonic Mucosa of Patients With Diarrhea\u2010Predominant Irritable Bowel Syndrome"],"prefix":"10.1002","volume":"40","author":[{"given":"Julien","family":"Bertrand","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ibtissem","family":"Ghouzali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charl\u00e8ne","family":"Gu\u00e9rin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"B\u00f4le\u2010Feysot","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e9lodie","family":"Gouteux","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"D\u00e9chelotte","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Ducrott\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mo\u00efse","family":"Co\u00ebffier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,5,13]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2005.11.061"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.45.2008.ii43"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1053\/gast.2002.37095"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2009.181834"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pain.2009.06.017"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/ajg.2011.257"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/ajg.2009.700"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2007.140806"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/ajg.2011.472"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10620-006-9473-x"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.CCM.0000162680.52397.97"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00726-010-0814-x"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00459.2002"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00012.2004"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clnu.2013.01.014"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clnu.2013.09.003"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clnu.2006.07.003"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.48.1.28"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2015.02.057"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0261-5614(03)00040-2"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0042777"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.2006.00672.x"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2014.09.037"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(76)90366-7"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.2009.27462S"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.201400304"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/ibd.20543"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0025-6196(12)62270-8"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/ibd.21017"},{"key":"e_1_2_8_32_1","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1093\/jn\/123.2.234","article-title":"Metabolic characteristics of pig colonocytes after adaptation to a high fiber diet","volume":"123","author":"Darcy\u2010Vrillon B","year":"1993","journal-title":"J Nutr"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v17.i12.1569"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00058.2004"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2003.11.055"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10753-010-9183-6"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.49.5.636"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2008.150425"}],"container-title":["Journal of Parenteral and Enteral Nutrition"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0148607115587330","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/0148607115587330","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/aspenjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1177\/0148607115587330","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T10:59:16Z","timestamp":1760871556000},"score":1,"resource":{"primary":{"URL":"https:\/\/aspenjournals.onlinelibrary.wiley.com\/doi\/10.1177\/0148607115587330"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,5,13]]},"references-count":37,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2016,11]]}},"alternative-id":["10.1177\/0148607115587330"],"URL":"https:\/\/doi.org\/10.1177\/0148607115587330","archive":["Portico"],"relation":{},"ISSN":["0148-6071","1941-2444"],"issn-type":[{"value":"0148-6071","type":"print"},{"value":"1941-2444","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,5,13]]}}}