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Furthermore, we aim to determine if this adaptive effect was potentiated with enteral nutrition (EN). <jats:italic>Methods:<\/jats:italic> Neonatal piglets were block\u2010randomized to 75% mid\u2010intestinal (JI group, retains ileum) or distal\u2010intestinal (JC group, has no ileum) resection or no resection (sham control) and GLP\u20102 treatment (11 nmol\/kg\/d) or saline control for 7 days. Piglets received nutrition support, either 100% parenteral nutrition (PN; 0% EN, n = 32 in total) or 80% PN + 40% EN (n = 28 in total). Adaptation was assessed by morphological and histological changes, as well as RT quantitative polymerase chain reaction of nutrient transporters and tight junctional proteins and fat absorption. Data are analyzed by 3\u2010way analysis of variance (ANOVA) and 2\u2010way ANOVA per EN level. <jats:italic>Results:<\/jats:italic> GLP\u20102 treatment lengthened villi, deepened crypts, and improved intestinal weight in the remnant intestine of JC piglets. EN was a more potent adaptive stimulus for JI piglets. Small intestinal lengthening occurred only in the JI group, when given EN. There was no difference in total fat absorption and messenger RNA expression of nutrient transporters and tight junctional proteins. <jats:italic>Conclusions:<\/jats:italic> GLP\u20102 administration augmented structural adaptation in JC piglets with distal intestinal resection. Given JI anatomy, further stimulation by GLP\u20102 treatment over innate adaptation and stimulation by EN was modest and restricted to ileum. The differential effect of GLP\u20102 in neonatal SBS, depending on remnant anatomy, has important implications for clinical translation and planning of clinical trials.<\/jats:p>","DOI":"10.1177\/0148607116665812","type":"journal-article","created":{"date-parts":[[2016,9,22]],"date-time":"2016-09-22T23:18:50Z","timestamp":1474586330000},"page":"156-170","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":12,"title":["Differential Effects on Intestinal Adaptation Following Exogenous Glucagon\u2010Like Peptide 2 Therapy With and Without Enteral Nutrition in Neonatal Short Bowel Syndrome"],"prefix":"10.1002","volume":"41","author":[{"given":"David 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