{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T17:18:39Z","timestamp":1780507119304,"version":"3.54.1"},"reference-count":41,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[1995,5,1]],"date-time":"1995-05-01T00:00:00Z","timestamp":799286400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Parenter Enteral Nutr"],"published-print":{"date-parts":[[1995,5]]},"abstract":"<jats:p><jats:italic>Background:<\/jats:italic> A high\u2010fat, low\u2010fiber diet resulting in increased excretion of fecal secondary bile acids is regarded as a major risk factor for colon cancer. Incubation of human colonic biopsies with the secondary bile acid deoxycholic acid (DCA) leads to hyperproliferation with expansion of the proliferative zone, ie, a biomarker of increased cancer risk. Antiproliferative effects on various colon cancer cell lines, however, were reported for butyrate (BUT), a fermentation product of dietary fiber. <jats:italic>Methods:<\/jats:italic> In the following <jats:italic>in vitro<\/jats:italic> study we incubated biopsies from the normal sigmoid colon of 12 patients (age 55.8 \u00b1 3.6 years) with 5 \u03bcM DCA or a combination of 5 \u03bcM DCA plus 10 mM BUT (DCA\/BUT) and determined epithelial proliferation by bromodeoxyuridine immunohistochemistry. As a possible mediator for the DCA effects on colonic cell proliferation, mucosal prostaglandin E<jats:sub>2<\/jats:sub> (PGE<jats:sub>2<\/jats:sub>) release into the incubation medium was measured by <jats:sup>125<\/jats:sup>I\u2010PGE<jats:sub>2<\/jats:sub> radioimmunoassay. <jats:italic>Results:<\/jats:italic> Incubation with DCA alone revealed a significantly higher labeling index for the whole crypt (.17 vs.11, p &lt;.01) and for the upper 40% of the crypt (.05 vs.01, p &lt;.01) compared with DCA\/BUT. Mucosal PGE2 release during DCA\/BUT incubation showed a trend toward lower values compared with DCA incubation (357.07 vs 434.29 pg\/mg per hour; p =.07). <jats:italic>Conclusion:<\/jats:italic> The results indicate a normalization of DCA\u2010induced hyperproliferation of colonic epithelium by butyrate that is not clearly mediated by PGE<jats:sub>2<\/jats:sub>. Considering that nutrition affects the luminal concentrations of DCA and butyrate, our findings may have implications for colonic carcinogenesis. <jats:italic>(Journal<\/jats:italic> of <jats:italic>Parenteral and Enteral Nutrition<\/jats:italic> <jats:bold>19:<\/jats:bold>182\u2013186, 1995)<\/jats:p>","DOI":"10.1177\/0148607195019003182","type":"journal-article","created":{"date-parts":[[2008,5,14]],"date-time":"2008-05-14T19:51:26Z","timestamp":1210794686000},"page":"182-186","source":"Crossref","is-referenced-by-count":24,"title":["Effects of Deoxycholic Acid and Butyrate on Mucosal Prostaglandin E2 Release and Cell Proliferation in the Human Sigmoid Colon"],"prefix":"10.1002","volume":"19","author":[{"given":"Hans\u2010Peter","family":"Bartram","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolfgang","family":"Scheppach","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Englert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerda","family":"Dusel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Astrid","family":"Richter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"Richter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heinrich","family":"Kasper","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[1995,5]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19920901)70:3 <1206::AID-CNCR2820701504>3.0.CO;2-J"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-086380-8.50026-0"},{"key":"e_1_2_1_4_1","first-page":"4629","article-title":"Biochemical epidemiology of colon cancer: effect of types of dietary fiber on fecal mutagens, acid, and neutral sterols in healthy subjects","volume":"49","author":"Reddy B.","year":"1989","journal-title":"Cancer Res"},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1007\/BF02988541","article-title":"Fiber and cancer","volume":"7","author":"Kritchevsky D.","year":"1990","journal-title":"Med Oncol Tumor Pharmacother"},{"key":"e_1_2_1_6_1","first-page":"601","article-title":"Dietary fibre, fermentation and large bowel cancer","volume":"6","author":"Cummings JH","year":"1987","journal-title":"Cancer Surv"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/50.6.1437"},{"key":"e_1_2_1_8_1","first-page":"453","volume-title":"Carcinogenesis","author":"Reddy BS","year":"1978"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.3109\/00365528809093945"},{"key":"e_1_2_1_10_1","first-page":"4238","article-title":"Effect of starch malabsorption on fecal bile acids and neutral sterols in humans: possible implications for colonic carcinogenesis","volume":"51","author":"Bartram HP","year":"1991","journal-title":"Cancer Res"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1080\/01635589109514150"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.27.12.1457"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19800315)45:5 <1185::AID-CNCR2820451324>3.0.CO;2-W"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1177\/014860719201600143"},{"key":"e_1_2_1_15_1","first-page":"148","volume-title":"Short Chain Fatty Acids (Falk Symposium 73)","author":"Young GP","year":"1994"},{"key":"e_1_2_1_16_1","first-page":"235","article-title":"Biomarkers of increased susceptibility to gastrointestinal cancer: new application to studies of cancer prevention in human subjects","volume":"48","author":"Lipkin M.","year":"1988","journal-title":"Cancer Res"},{"key":"e_1_2_1_17_1","first-page":"133","volume-title":"Colorectal Cancer: Prevention, Epidemiology, and Screening","author":"Deschner EE","year":"1980"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1159\/000198801"},{"key":"e_1_2_1_19_1","first-page":"3283","article-title":"Proliferation of human colonic mucosa as an intermediate biomarker of carcinogenesis: effects of butyrate, deoxycholate, calcium, ammonia, and pH","volume":"53","author":"Bartram HP","year":"1993","journal-title":"Cancer Res"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1042\/cs0610641"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1980.6"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3835(89)90036-0"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19881201)62:11<2373::AID-CNCR2820621120>3.0.CO;2-Y"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19880601)61:11<2286::AID-CNCR2820611124>3.0.CO;2-K"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(89)91549-7"},{"key":"e_1_2_1_26_1","first-page":"S129","article-title":"Co\u2010culture of colonic mucosa: a novel technique for investigating the effect of soluble mediators in inflammatory bowel disease","volume":"2","author":"Wardle TD","year":"1990","journal-title":"Eur J Gastroenterol Hepatol"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(93)90135-Y"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3835(87)90158-3"},{"key":"e_1_2_1_29_1","first-page":"1917","article-title":"Correlations between rectal mucosa cell proliferation and the clinical and pathological features of nonfamilial neoplasia of the large intestine","volume":"51","author":"Risio M.","year":"1991","journal-title":"Cancer Res"},{"key":"e_1_2_1_30_1","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/S0889-8588(18)30566-5","article-title":"Dietary epidemiology of colon cancer","volume":"3","author":"Vogel VG","year":"1989","journal-title":"Hematol Oncol Clin North Am"},{"key":"e_1_2_1_31_1","first-page":"149","article-title":"The relationship between faecal bile acids and the development of experimental colon cancer","volume":"69","author":"Barton T.","year":"1988","journal-title":"Br J Exp Pathol"},{"key":"e_1_2_1_32_1","first-page":"1129","article-title":"Increase by deoxycholic acid of the colonic nuclear damage induced by known carcinogens in C57BL\/6J mice","volume":"76","author":"Suzuki K.","year":"1986","journal-title":"J Natl Cancer Inst"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1984.98"},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/78.1.107"},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1177\/0148607189013002109"},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1079\/BJN19870073"},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.22.9.763"},{"key":"e_1_2_1_38_1","first-page":"3321","article-title":"Heterogeneity in the induction and expression of carcinoembryonic antigen\u2010related antigens in human colon cancer cell lines","volume":"49","author":"Toribara NW","year":"1989","journal-title":"Cancer Res"},{"key":"e_1_2_1_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3835(90)90160-Y"},{"key":"e_1_2_1_40_1","doi-asserted-by":"publisher","DOI":"10.1080\/01635589109514161"},{"key":"e_1_2_1_41_1","first-page":"4790","article-title":"Effect of dietary eicosapentaenoic acid on azoxymethane\u2010induced colon carcinogenesis in rats","volume":"48","author":"Minoura T.","year":"1988","journal-title":"Cancer Res"},{"key":"e_1_2_1_42_1","doi-asserted-by":"crossref","first-page":"1342","DOI":"10.1681\/ASN.V371342","article-title":"Species\u2010specific properties of the glomerular mesangium","volume":"3","author":"Sraer JD","year":"1993","journal-title":"J Am Soc Nephrol"}],"container-title":["Journal of Parenteral and Enteral Nutrition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aspenjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1177\/0148607195019003182","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T18:35:56Z","timestamp":1760639756000},"score":1,"resource":{"primary":{"URL":"https:\/\/aspenjournals.onlinelibrary.wiley.com\/doi\/10.1177\/0148607195019003182"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,5]]},"references-count":41,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1995,5]]}},"alternative-id":["10.1177\/0148607195019003182"],"URL":"https:\/\/doi.org\/10.1177\/0148607195019003182","archive":["Portico"],"relation":{},"ISSN":["0148-6071","1941-2444"],"issn-type":[{"value":"0148-6071","type":"print"},{"value":"1941-2444","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,5]]}}}