{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T11:12:53Z","timestamp":1789729973235,"version":"4.0.1"},"reference-count":40,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[1997,9,1]],"date-time":"1997-09-01T00:00:00Z","timestamp":873072000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Parenter Enteral Nutr"],"published-print":{"date-parts":[[1997,9]]},"abstract":"<jats:p><jats:italic>Background:<\/jats:italic> Bum injury is associated with substantial whole\u2010body protein loss, reflecting mainly a catabolic response in skeletal muscle. Recent studies suggest that treatment with insulin\u2010like growth factor 1 (IGF\u20101) may reverse the catabolic response to burn injury, but the effects of IGF\u20101 on muscle protein synthesis and breakdown rates after bum injury are not known. We tested the hypothesis that IGF\u20101 blunts the catabolic response in skeletal muscle after burn injury by stimulating protein synthesis and inhibiting protein breakdown and that this effect of IGF\u20101 is caused by a direct effect on muscle tissue. <jats:italic>Methods:<\/jats:italic> Intact extensor digitorum longus muscles from burned, sham\u2010burned, and untreated rats were incubated in the absence or presence of different concentrations of IGF\u20101. Total and myofibrillar protein breakdown rates were measured as net release of tyrosine and 3\u2010methylhistidine, respectively. Protein synthesis rates were determined by measuring the incorporation of (U\u2010<jats:sup>14<\/jats:sup>C)\u2010phenylalanine into protein. Results: IGF\u20101 stimulated protein synthesis and inhibited protein breakdown in a dose\u2010dependent fashion in muscles from burned and unburned rats. The maximal effect of IGF\u20101 on protein synthesis was seen at a hormone concentration of 100 ng\/mL, whereas protein breakdown was further inhibited when the hormone concentration was increased to 1 \u03bcg\/mL. Ubiquitin messenger RNA (mRNA) levels were reduced by IGF\u20101 in incubated muscles, suggesting that IGF\u20101 may inhibit ubiquitin\u2010dependent protein breakdown. <jats:italic>Conclusions:<\/jats:italic> These results suggest that the anabolic effects of IGF\u20101 after burn may reflect inhibited protein breakdown and stimulated protein synthesis in skeletal muscle and that this response may be caused by a direct effect of IGF\u20101 on muscle tissue. <jats:italic>(journal<\/jats:italic> of <jats:italic>Parenteral and Enteral Nutrition<\/jats:italic> 21:245\u2013251, 1997)<\/jats:p>","DOI":"10.1177\/0148607197021005245","type":"journal-article","created":{"date-parts":[[2008,5,14]],"date-time":"2008-05-14T19:51:26Z","timestamp":1210794686000},"page":"245-251","source":"Crossref","is-referenced-by-count":48,"title":["Insulin\u2010Like Growth Factor 1 Stimulates Protein Synthesis and Inhibits Protein Breakdown in Muscle From Burned 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