{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T09:24:05Z","timestamp":1790760245062,"version":"4.1.0"},"reference-count":28,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2011,5,31]],"date-time":"2011-05-31T00:00:00Z","timestamp":1306800000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Toxicol Pathol"],"published-print":{"date-parts":[[2011,8]]},"abstract":"<jats:p>The C57BL\/6 mouse strain (or derivation of this strain) is used as a background for many transgenic mouse models. This strain has a relatively low susceptibility to chemically induced hepatocarcinogenesis compared with other commonly used experimental mouse strains. In the present study, the authors treated C57BL\/6 mice with 25, 50, and 75 mg\/kg of diethylnitrosamine (DEN) for 4 or 8 weeks by intraperitoneal injection to investigate the dose-response pattern of preneoplastic and neoplastic lesion formation in the liver. DEN induced preneoplastic lesions and cytokeratin 8\/18\u2013positive foci in a dose-dependent manner. In the 75 mg\/kg for 8 weeks treatment group, hepatocellular adenoma, cholangioma and hemangioma, and cytokeratin 19\u2013positive foci were also induced, but a significant decrease in body weight was observed. The suitable DEN treatment range for this strain was concluded to be from 75 mg\/kg for 4 weeks (total amount = 300 mg\/kg) to 50 mg\/kg for 8 weeks (total amount = 400 mg\/kg). These results should prove useful for future studies investigating hepatocarcinogenesis in both the background C57BL\/6 strain and other transgenic mouse models derived from it.<\/jats:p>","DOI":"10.1177\/0192623311409596","type":"journal-article","created":{"date-parts":[[2011,6,1]],"date-time":"2011-06-01T01:18:48Z","timestamp":1306891128000},"page":"776-786","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":39,"title":["Dose-Related Induction of Hepatic Preneoplastic Lesions by Diethylnitrosamine in C57BL\/6 Mice"],"prefix":"10.1177","volume":"39","author":[{"given":"Masahiko","family":"Kushida","sequence":"first","affiliation":[{"name":"Department of Environmental Health, School of Public Health, Indiana University, Bloomington, Indiana, USA"},{"name":"Environmental Health Science Laboratory, Sumitomo Chemical Co., Ltd., Osaka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa M.","family":"Kamendulis","sequence":"additional","affiliation":[{"name":"Department of Environmental Health, School of Public Health, Indiana University, Bloomington, Indiana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tyler J.","family":"Peat","sequence":"additional","affiliation":[{"name":"Department of Environmental Health, School of Public Health, Indiana University, Bloomington, Indiana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James E.","family":"Klaunig","sequence":"additional","affiliation":[{"name":"Department of Environmental Health, School of Public Health, Indiana University, Bloomington, Indiana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2011,5,31]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.23488"},{"key":"e_1_3_3_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfi129"},{"key":"e_1_3_3_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/7.2.215"},{"key":"e_1_3_3_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3835(84)90157-5"},{"key":"e_1_3_3_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/7.10.1701"},{"key":"e_1_3_3_7_1","doi-asserted-by":"publisher","DOI":"10.1081\/DMR-100100575"},{"key":"e_1_3_3_8_1","doi-asserted-by":"publisher","DOI":"10.1289\/ehp.8350149"},{"key":"e_1_3_3_9_1","first-page":"6470","article-title":"Chronic nitrosamine ingestion in 1040 rodents: The effect of the choice of nitrosamine, the species studied, and the age of starting exposure","volume":"51","author":"Gray R.","year":"1991","unstructured":"Gray R., Peto R., Brantom P., Grasso P. 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