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The acronym DMBDD stands for the names of five genotoxic agents used for initiation of multiorgan carcinogenesis. The Brazilian Agency for the Environment officially recognized a variation of this assay (DMBDD\n                    <jats:sup>b<\/jats:sup>\n                    ) as a valid method to assess the carcinogenic potential of agrochemicals. Different from the original protocol, this DMBDD\n                    <jats:sup>b<\/jats:sup>\n                    is 30-week long, uses Wistar rats and two positive control groups exposed to carcinogenesis promoters sodium phenobarbital (PB) or 2-acetylaminofluorene (2-AAF). This report presents the experience of an academic laboratory with the DMBDD\n                    <jats:sup>b<\/jats:sup>\n                    assay and contributes to the establishment of this alternative DMBDD bioassay in a different rat strain. Frequent lesions observed in positive groups to evaluate the promoting potential of pesticides and the immunohistochemical expressions of liver cytochrome P450 (CYP) 2B1\/2B2 and CYP1A2 enzymes were assessed. Commonly affected organs were liver, kidney, intestines, urinary bladder, and thyroid. PB promoting activity was less evident than that of 2-AAF, especially in males. This study provides a repository of characteristic lesions occurring in positive control animals submitted to a modified alternative 2-stage multiorgan protocol for carcinogenesis in Wistar rat.\n                  <\/jats:p>","DOI":"10.1177\/0192623316678931","type":"journal-article","created":{"date-parts":[[2017,1,5]],"date-time":"2017-01-05T14:22:39Z","timestamp":1483626159000},"page":"1146-1159","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":5,"title":["Alternative Multiorgan Initiation\u2013Promotion Assay for Chemical Carcinogenesis in the Wistar Rat"],"prefix":"10.1177","volume":"44","author":[{"given":"Marize de Lourdes Marzo","family":"Solano","sequence":"first","affiliation":[{"name":"Department of Pathology, Botucatu Medical School, UNESP\u2014S\u00e3o Paulo State University, Botucatu, S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noeme Souza","family":"Rocha","sequence":"additional","affiliation":[{"name":"Department of Clinical Medicine, Faculty of Veterinary Medicine and Zootechnology, UNESP\u2014S\u00e3o Paulo State University, Botucatu, S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis Fernando","family":"Barbisan","sequence":"additional","affiliation":[{"name":"Department of Morphology, Bioscience Institute, UNESP\u2014S\u00e3o Paulo State University, Botucatu, S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carla Adriene da Silva","family":"Franchi","sequence":"additional","affiliation":[{"name":"Department of Pathology, Botucatu Medical School, UNESP\u2014S\u00e3o Paulo State University, Botucatu, S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana L\u00facia Tozzi","family":"Spinardi-Barbisan","sequence":"additional","affiliation":[{"name":"Department of Pathology, Botucatu Medical School, UNESP\u2014S\u00e3o Paulo State University, Botucatu, S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Luiza Cotrim Sartor","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Department of Pathology, Botucatu Medical School, UNESP\u2014S\u00e3o Paulo State University, Botucatu, S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daisy Maria F\u00e1vero","family":"Salvadori","sequence":"additional","affiliation":[{"name":"Department of Pathology, Botucatu Medical School, UNESP\u2014S\u00e3o Paulo State University, Botucatu, S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L\u00facia Regina","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Department of Pathology, Botucatu Medical School, UNESP\u2014S\u00e3o Paulo State University, Botucatu, S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jo\u00e3o Lauro Viana","family":"de Camargo","sequence":"additional","affiliation":[{"name":"Department of Pathology, Botucatu Medical School, UNESP\u2014S\u00e3o Paulo State University, Botucatu, S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2016,12,1]]},"reference":[{"key":"bibr1-0192623316678931","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-074901-0.50021-6"},{"key":"bibr2-0192623316678931","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.1989.tb01630.x"},{"key":"bibr3-0192623316678931","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.1989.tb02256.x"},{"key":"bibr4-0192623316678931","first-page":"3","volume-title":"Monographs on Pathology of Laboratory Animals: Digestive System. 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