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Treatment of NSCLC bone metastases, which are often multiple, is palliative, and the median survival times are 3 to 6 months. We have characterized spontaneous vertebral metastases in a brain metastases model of NSCLC and correlated these findings with epithelial\u2013mesenchymal transition (EMT). Brain metastases were established in athymic nude mice following intracardiac injection of brain-seeking adenocarcinoma NSCLC cells. Thirty-nine percent of mice (14\/36) developed spontaneous vertebral metastases, spinal cord compression, and hind-limb paralysis. Vertebral metastases consisted of an adenocarcinoma phenotype with neoplastic epithelial cells arranged in cords or acini and a mesenchymal phenotype with spindloid neoplastic cells arranged in bundles and streams. Quantitative and qualitative immunohistochemical and immunofluorescence assays demonstrated an increase in vimentin expression compared to cytokeratin expression in vertebral metastases. A correlation with EMT was supported by an increase in CD44 in vertebral metastases and parenchymal metastases. These data demonstrate a translational lung cancer metastasis model with spontaneous vertebral metastasis. The mesenchymal and epithelial phenotype of these spontaneous metastases coupled with EMT provide a conduit to improve drug delivery and overall patient survival.<\/jats:p>","DOI":"10.1177\/0192623319838491","type":"journal-article","created":{"date-parts":[[2019,5,7]],"date-time":"2019-05-07T23:52:48Z","timestamp":1557273168000},"page":"515-527","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":5,"title":["Epithelial\u2013mesenchymal Transition Phenotypes in Vertebral Metastases of Lung Cancer"],"prefix":"10.1177","volume":"47","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7819-1503","authenticated-orcid":false,"given":"Alexandra M.","family":"Dieterly","sequence":"first","affiliation":[{"name":"Department of Comparative Pathobiology, Purdue University College of Veterinary Medicine, West Lafayette, Indiana, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gozde","family":"Uzunalli","sequence":"additional","affiliation":[{"name":"Department of Comparative Pathobiology, Purdue University College of Veterinary Medicine, West Lafayette, Indiana, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chinyere M.","family":"Kemet","sequence":"additional","affiliation":[{"name":"Department of Comparative Pathobiology, Purdue University College of Veterinary Medicine, West Lafayette, Indiana, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arvin H.","family":"Soepriatna","sequence":"additional","affiliation":[{"name":"Weldon School of Biomedical Engineering, Purdue University College of Engineering, West Lafayette, Indiana, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Craig J.","family":"Goergen","sequence":"additional","affiliation":[{"name":"Weldon School of Biomedical Engineering, Purdue University College of Engineering, West Lafayette, Indiana, USA"},{"name":"Purdue University Center for Cancer Research, West Lafayette, Indiana, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. 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