{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T17:06:00Z","timestamp":1777914360583,"version":"3.51.4"},"reference-count":28,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[1987,1,1]],"date-time":"1987-01-01T00:00:00Z","timestamp":536457600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxicol Pathol"],"published-print":{"date-parts":[[1987,1]]},"abstract":"<jats:p>\n                    It has been observed that human lymphocytes (HL) and fibroblasts, isolated in vitro from donors carrying the Mediterranean variant of glucose-6-phosphate dehydrogenase (G6PD), show a great decrease in this enzymatic activity, the hexose monophosphate shunt, and the NADPH\/NADP\n                    <jats:sup>+<\/jats:sup>\n                    ratio. This effect is associated with a decreased sensitivity of G6PD-deficient cells to the benzo(a)pyrene (BaP) cytotoxic effect and to a decreased in vitro transformation of BaP-treated fibroblasts. Further, benzo(a)anthracene (BaA)-induced BaP hydroxylase activity is lower in G6PD-deficient cells, when measured in the presence of endogenous NADPH. It has been hypothesized that the NADPH level could be rate-limiting for the NADPH-dependent steps of BaP metabolic activation. To test this hypothesis, the formation of BaP metabolites was studied in normal and G6PD-deficient HL incubated with the carcinogen. HPLC profiles of organic-soluble metabolites revealed that both types of HL produced all the following known BaP metabolites: 9,10-, 4,5- and 7,8-dihydrodiols, quinones, 9- and 3-hydroxy and two peaks of more polar metabolites. There was a great decrease of the various metabolites in the deficient HL. A decrease of total water-soluble BaP metabolites also occurred. HL formed mutagenic metabolites for S. typhimurium TA100 (his-) when incubated with the rat liver S9 fraction. When intact HL substituted S9 fraction, a significant reversed mutation occurred only with normal HL. This could indicate that the NADPH pool is inadequate in G6PD-deficient HL for active BaP metabolism. Accordingly, deficient HL formed lower amounts of BaP:DNA adducts than control during incubation with BaP.\n                  <\/jats:p>","DOI":"10.1177\/019262338701500118","type":"journal-article","created":{"date-parts":[[2009,9,14]],"date-time":"2009-09-14T21:30:11Z","timestamp":1252963811000},"page":"115-119","source":"Crossref","is-referenced-by-count":5,"title":["Control of Glucose-6-Phosphate Dehydrogenase Deficiency on the Formation of Mutagenic and Carcinogenic Metabolites Derived from Benzo(a)pyrene"],"prefix":"10.1177","volume":"15","author":[{"given":"Lucia","family":"Pirisi","sequence":"first","affiliation":[{"name":"Istituto di Patologia Generate dell'Universit\u00e0 di Sassari, Via P. Manzella 4, 07100 Sassari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renato","family":"Garcea","sequence":"additional","affiliation":[{"name":"Istituto di Patologia Generate dell'Universit\u00e0 di Sassari, Via P. Manzella 4, 07100 Sassari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosa","family":"Pascale","sequence":"additional","affiliation":[{"name":"Istituto di Patologia Generate dell'Universit\u00e0 di Sassari, Via P. Manzella 4, 07100 Sassari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Emilia","family":"Ruggiu","sequence":"additional","affiliation":[{"name":"Istituto di Patologia Generate dell'Universit\u00e0 di Sassari, Via P. Manzella 4, 07100 Sassari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesco","family":"Feo","sequence":"additional","affiliation":[{"name":"Istituto di Patologia Generate dell'Universit\u00e0 di Sassari, Via P. Manzella 4, 07100 Sassari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[1987,1,1]]},"reference":[{"key":"atypb1","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-159-40294"},{"key":"atypb2","first-page":"934","volume":"42","author":"Autrup H","year":"1982","journal-title":"Cancer Res."},{"key":"atypb3","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910280319"},{"key":"atypb4","doi-asserted-by":"publisher","DOI":"10.1016\/0009-2797(76)90014-4"},{"key":"atypb5","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/6.7.1017"},{"key":"atypb6","doi-asserted-by":"publisher","DOI":"10.1159\/000224280"},{"key":"atypb7","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910240202"},{"key":"atypb8","first-page":"3419","volume":"38","author":"Feo F","year":"1984","journal-title":"Cancer Res."},{"key":"atypb9","first-page":"262","volume":"12","author":"Feo F","year":"1984","journal-title":"Toxicol. Pathol."},{"key":"atypb10","first-page":"459","volume-title":"Membranes in Tumour Growth","author":"Feo F","year":"1982"},{"key":"atypb11","doi-asserted-by":"publisher","DOI":"10.1177\/019262338701500206"},{"key":"atypb12","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1980.60.4.1107"},{"key":"atypb13","doi-asserted-by":"publisher","DOI":"10.1126\/science.982061"},{"key":"atypb14","first-page":"327","volume":"25","author":"Kellermann G","year":"1973","journal-title":"Amer. J. Hum. Genet."},{"key":"atypb15","first-page":"1859","volume":"42","author":"Kuroki T","year":"1982","journal-title":"Cancer Res."},{"key":"atypb16","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-142-37001"},{"key":"atypb17","first-page":"17","volume":"55","author":"Mbensa M","year":"1978","journal-title":"East African Med. 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Pharmacol."},{"key":"atypb24","first-page":"1199","volume":"39","author":"Prough RA","year":"1979","journal-title":"Cancer Res."},{"key":"atypb25","first-page":"2482","volume":"35","author":"Schwartz AG","year":"1975","journal-title":"Cancer Res."},{"key":"atypb26","first-page":"3651","volume":"35","author":"Selkirk JK","year":"1975","journal-title":"Cancer Res."},{"key":"atypb27","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(72)91416-X"},{"key":"atypb28","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3835(78)80023-8"}],"container-title":["Toxicologic Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/019262338701500118","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/019262338701500118","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T16:49:19Z","timestamp":1777654159000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/019262338701500118"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,1]]},"references-count":28,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1987,1]]}},"alternative-id":["10.1177\/019262338701500118"],"URL":"https:\/\/doi.org\/10.1177\/019262338701500118","relation":{},"ISSN":["0192-6233","1533-1601"],"issn-type":[{"value":"0192-6233","type":"print"},{"value":"1533-1601","type":"electronic"}],"subject":[],"published":{"date-parts":[[1987,1]]}}}