{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T20:54:25Z","timestamp":1791233665413,"version":"4.1.0"},"reference-count":25,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[1995,9,1]],"date-time":"1995-09-01T00:00:00Z","timestamp":809913600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxicol Pathol"],"published-print":{"date-parts":[[1995,9]]},"abstract":"<jats:p>Groups of C57BL6 mice of each sex were assigned to one of 2 dietary regimens, ad libitum (AL) or dietary restriction (DR), to study effects of food restriction on body weight, survival, and neoplasia. The AL and DR groups were subdivided into a scheduled sacrifice group for examination at 6-mo intervals, and a lifetime group to provide longevity data. Necropsies and microscopic examinations were conducted on 911 animals. In the lifetime group food consumption averaged 33.6 and 34.4 g per week by AL males and AL females, respectively; the DR counterparts were given 40% less. The diet contained 4.35 kcal\/g. The average lifetime body weights were 34.8, 26.8, 22.6, and 21.6 g for AL males, AL females, DR males, and DR females, respectively, and their age at 50% survival was 27.5, 26.9, 31.7, and 33.5 mo. Maximal lifespan was increased 18% in DR males and females. Lifetime incidence of tumor-bearing mice was 89% and 86% for AL males and females, versus 64% for each sex of DR mice. Dramatic reduction occurred in female DR mice in lymphoma (9% vs 29%), pituitary neoplasms (1% vs 37%), and thyroid neoplasms (0.4% vs 8%). In males, hepatocellular tumors were reduced to 1% from 10% by DR. In contrast, the incidence of histiocytic sarcoma was increased in DR females and unaffected in DR males. Tumor onset was delayed in DR animals; 87% of all neoplasms in males and 95% in females had occurred in the AL mice by 24 mo, whereas the DR animals had only 52% and 39% of their lifetime incidence, respectively, by that age. This study provided comparative AL and DR data from C57BL6 mice examined randomly at 6-mo intervals (cross-sectional group) in parallel with data from animals in similar cohort that was unsampled and allowed to succumb naturally (longevity group). Dietary restriction reduced the lifetime percentage of tumor-bearing animals and the number of tumors per animal, and delayed the age at onset of most neoplasms.<\/jats:p>","DOI":"10.1177\/019262339502300503","type":"journal-article","created":{"date-parts":[[2009,4,1]],"date-time":"2009-04-01T19:16:47Z","timestamp":1238613407000},"page":"570-582","source":"Crossref","is-referenced-by-count":167,"title":["Longevity, Body Weight, and Neoplasia in Ad Libitum-Fed and Diet-Restricted C57BL6 Mice Fed NIH-31 Open Formula Diet"],"prefix":"10.1177","volume":"23","author":[{"given":"Boon-Nam","family":"Blackwell","sequence":"first","affiliation":[{"name":"Pathology Associates, Inc., 3900 NCTR Road, Jefferson, Arkansas 72079"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas J.","family":"Bucci","sequence":"additional","affiliation":[{"name":"Pathology Associates, Inc., 3900 NCTR Road, Jefferson, Arkansas 72079"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronald W.","family":"Hart","sequence":"additional","affiliation":[{"name":"National Center for Toxicological Research, 3900 NCTR Road, Jefferson, Arkansas 72079"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angelo","family":"Turturro","sequence":"additional","affiliation":[{"name":"National Center for Toxicological Research, 3900 NCTR Road, Jefferson, Arkansas 72079"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[1995,9,1]]},"reference":[{"key":"atypb1","doi-asserted-by":"publisher","DOI":"10.1177\/030098589403100301"},{"key":"atypb2","first-page":"29","volume":"21","author":"Bucci TJ","year":"1992","journal-title":"Lab. 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Pathol"},{"key":"atypb11","volume-title":"Clinical biochemistry of the aging mouse","author":"Loeb WF","year":"1994"},{"key":"atypb12","first-page":"148","volume":"28","author":"Roe FJC","year":"1994","journal-title":"Anim"},{"key":"atypb13","volume-title":"Effect of ad libitum feeding and forty percent food restriction on body weight, longevity and neoplasia in B5C3F1, C57BL6, and B6D2F1, mice","author":"Sheldon W.","year":"1994"},{"key":"atypb14","doi-asserted-by":"publisher","DOI":"10.1177\/019262339502300403"},{"key":"atypb15","first-page":"448","volume":"29","author":"Sheldon W.","year":"1992","journal-title":"Vet. Pathol"},{"key":"atypb16","doi-asserted-by":"publisher","DOI":"10.1093\/geronj\/21.3.404"},{"key":"atypb17","first-page":"892","volume":"21","author":"Stowe HD","year":"1971","journal-title":"Lab. Anim. 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Academic Press, pp. 11-36."}],"container-title":["Toxicologic Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/019262339502300503","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/019262339502300503","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T16:55:08Z","timestamp":1777654508000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/019262339502300503"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,9]]},"references-count":25,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1995,9]]}},"alternative-id":["10.1177\/019262339502300503"],"URL":"https:\/\/doi.org\/10.1177\/019262339502300503","relation":{},"ISSN":["0192-6233","1533-1601"],"issn-type":[{"value":"0192-6233","type":"print"},{"value":"1533-1601","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,9]]}}}