{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T16:41:16Z","timestamp":1777912876549,"version":"3.51.4"},"reference-count":30,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[1998,3,1]],"date-time":"1998-03-01T00:00:00Z","timestamp":888710400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxicol Pathol"],"published-print":{"date-parts":[[1998,3]]},"abstract":"<jats:p>\n                    TO test the applicability of an anti-8-hydroxy-2'-deoxyguanosine (8-OH-dG) antibody for immunohistochemistry using paraffin-embedded sections, carbon tetrachloride (CCl\n                    <jats:sub>4<\/jats:sub>\n                    )-induced rat liver injury was evaluated. Male rats were given a single dose of CCl\n                    <jats:sub>4<\/jats:sub>\n                    and killed at 6 hr, 12 hr, 1, 2, 3, and 7 days thereafter. Severe centrilobular necrosis was evident at 1 day. At 2 days, moderate mononuclear cell infiltration was present in centrilobular necrotic regions. Infiltrating mononuclear cells, surrounding sinusoidal endothelial cells and hepatocytes were stained with anti-8-OH-dG antibody at 2 and 3 days. Formation of 8-OH-dG in DNA and 8-oxo-dGTPase mRNA expression were also increased at these time points, the amounts of malondialdehyde and 4-hydroxy-2-nonenal showed 2 peaks at 6 hr and 3 days. The findings suggest that the main contributory factor in the massive hepatic necrosis was increased lipid peroxidation, rather than excessive formation of 8-OH-dG, and that the observed increase in the latter was largely due to infiltrating mononuclear cells. The agreement between biochemical data and the results for immunohistochemical analysis confirms that the anti-8-OH-dG antibody is applicable for detection of cells targeted by free radicals in paraffin-embedded sections and also for investigation of the mechanisms of oxidative damage-related disease, including carcinogenesis.\n                  <\/jats:p>","DOI":"10.1177\/019262339802600209","type":"journal-article","created":{"date-parts":[[2009,9,14]],"date-time":"2009-09-14T21:30:11Z","timestamp":1252963811000},"page":"247-252","source":"Crossref","is-referenced-by-count":27,"title":["Immunohistochemical Detection of 8-Hydroxy-2'-deoxyguanosine in Paraffin-Embedded Sections of Rat Liver after Carbon Tetrachloride Treatment"],"prefix":"10.1177","volume":"26","author":[{"given":"Satoru","family":"Takahashi","sequence":"first","affiliation":[{"name":"First Department of Pathology, Nagoya City University Medical School, 1 Kawasumi Mizuho-cho, Mizuho-ku, Nagoya 467, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masao","family":"Hirose","sequence":"additional","affiliation":[{"name":"First Department of Pathology, Nagoya City University Medical School, 1 Kawasumi Mizuho-cho, Mizuho-ku, Nagoya 467, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seiko","family":"Tamano","sequence":"additional","affiliation":[{"name":"Daiyukai Institute of Medical Science, 25 Niken-ya, Nishiazai, Azai-cho, Ichinomiya 491\u201301, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masakazu","family":"Ozaki","sequence":"additional","affiliation":[{"name":"First Department of Pathology, Nagoya City University Medical School, 1 Kawasumi Mizuho-cho, Mizuho-ku, Nagoya 467, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shin-Ichiro","family":"Orita","sequence":"additional","affiliation":[{"name":"First Department of Pathology, Nagoya City University Medical School, 1 Kawasumi Mizuho-cho, Mizuho-ku, Nagoya 467, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takayasu","family":"Ito","sequence":"additional","affiliation":[{"name":"First Department of Pathology, Nagoya City University Medical School, 1 Kawasumi Mizuho-cho, Mizuho-ku, Nagoya 467, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masao","family":"Takeuchi","sequence":"additional","affiliation":[{"name":"Japan Institute for the Control of Aging, 723\u20131, Haruoka, Fukuroi, Shizuoka 437\u201301, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hirotomo","family":"Ochi","sequence":"additional","affiliation":[{"name":"Japan Institute for the Control of Aging, 723\u20131, Haruoka, Fukuroi, Shizuoka 437\u201301, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shoko","family":"Fukada","sequence":"additional","affiliation":[{"name":"Department of Environmental Oncology, University of Occupational and Environmental Health, 1\u20131 Iseigaoka, Yahatanishi-ku, Kitakyushu 807, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Kasai","sequence":"additional","affiliation":[{"name":"Department of Environmental Oncology, University of Occupational and Environmental Health, 1\u20131 Iseigaoka, Yahatanishi-ku, Kitakyushu 807, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomoyuki","family":"Shirai","sequence":"additional","affiliation":[{"name":"First Department of Pathology, Nagoya City University Medical School, 1 Kawasumi Mizuho-cho, Mizuho-ku, Nagoya 467, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[1998,3,1]]},"reference":[{"key":"atypb1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V64.5.959.959"},{"key":"atypb2","doi-asserted-by":"publisher","DOI":"10.1016\/0891-5849(93)90005-F"},{"key":"atypb3","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/16.10.2343"},{"key":"atypb4","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/13.7.1127"},{"key":"atypb5","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(90)86134-H"},{"key":"atypb6","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(86)91156-3"},{"key":"atypb7","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(94)92211-X"},{"key":"atypb8","doi-asserted-by":"publisher","DOI":"10.1016\/0278-6915(95)00024-V"},{"key":"atypb9","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/7.11.1849"},{"key":"atypb10","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/12.4.2127"},{"key":"atypb11","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/7.5.813"},{"key":"atypb12","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/15.18.7638"},{"key":"atypb13","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.22.11021"},{"key":"atypb14","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3835(95)03980-B"},{"key":"atypb15","first-page":"129859a","volume":"117","author":"Okamoto S","year":"1992","journal-title":"Chem. Abst."},{"key":"atypb16","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-0348-7337-6_34"},{"key":"atypb17","doi-asserted-by":"publisher","DOI":"10.1016\/0009-3084(87)90063-6"},{"key":"atypb18","doi-asserted-by":"publisher","DOI":"10.1016\/0009-2797(81)90162-9"},{"key":"atypb19","first-page":"419","volume":"44","author":"Reubcr MD","year":"1970","journal-title":"J. Natl. Cancer Inst."},{"key":"atypb20","doi-asserted-by":"publisher","DOI":"10.1038\/349431a0"},{"key":"atypb21","doi-asserted-by":"publisher","DOI":"10.1042\/bj2220001"},{"key":"atypb22","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1183\/09031936.93.03070772","volume":"3","author":"Slosman DO","year":"1990","journal-title":"Eur. Respir. J."},{"key":"atypb23","doi-asserted-by":"publisher","DOI":"10.1002\/jlb.56.6.672"},{"key":"atypb24","first-page":"237","volume":"50","author":"Takagi A","year":"1991","journal-title":"Proc. Jpn. Cancer Assoc."},{"key":"atypb25","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1995.1230"},{"key":"atypb26","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V76.4.655.655"},{"key":"atypb27","doi-asserted-by":"publisher","DOI":"10.1055\/s-2008-1040483"},{"key":"atypb28","doi-asserted-by":"publisher","DOI":"10.1021\/bi00482a011"},{"key":"atypb29","first-page":"683","volume":"56","author":"Yarborough A","year":"1996","journal-title":"Cancer Res."},{"key":"atypb30","doi-asserted-by":"publisher","DOI":"10.1016\/0891-5849(95)00003-G"}],"container-title":["Toxicologic Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/019262339802600209","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/019262339802600209","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T16:06:52Z","timestamp":1777651612000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/019262339802600209"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,3]]},"references-count":30,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1998,3]]}},"alternative-id":["10.1177\/019262339802600209"],"URL":"https:\/\/doi.org\/10.1177\/019262339802600209","relation":{},"ISSN":["0192-6233","1533-1601"],"issn-type":[{"value":"0192-6233","type":"print"},{"value":"1533-1601","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,3]]}}}