{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T00:35:23Z","timestamp":1761006923820,"version":"build-2065373602"},"reference-count":21,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,7,19]],"date-time":"2016-07-19T00:00:00Z","timestamp":1468886400000},"content-version":"vor","delay-in-days":5527,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000072","name":"National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","award":["DE00430"],"award-info":[{"award-number":["DE00430"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["aao-hnsfjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Otolaryngol.--head neck surg."],"published-print":{"date-parts":[[2001,6]]},"abstract":"<jats:sec><jats:title>OBJECTIVE:<\/jats:title><jats:p>The goal of this study was to determine the genes required for head and neck cancer development.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN AND SETTING:<\/jats:title><jats:p>Differential mRNA display analysis was performed using human papillomavirus Type\u201016\u00a0infected immortalized human oral keratinocytes (HOK\u201016B) and its benzo(a)pyrene\u2010exposed tumorigenic derivative (HOK\u201016B\u2010BaP\u2010T).<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS:<\/jats:title><jats:p>Twenty\u2010one differentially expressed cDNA clones were identified between the 2 cell lines. Clone 4 with no known homology showed lower expression in tumorigenic cells compared with either normal or immortalized oral keratinocytes. Clone 6 expression was elevated in several head and neck cancer cells, in addition to Burkitt's lymphoma Rajl harboring latent Epstein\u2010Barr virus.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION:<\/jats:title><jats:p>These findings suggested that clone 6\u00a0may be involved in the oncogenic transformation whereas clone 4\u00a0may potentially function as a tumor suppressor gene.<\/jats:p><\/jats:sec><jats:sec><jats:title>SIGNIFICANCE:<\/jats:title><jats:p>Differential mRNA analysis using the in vitro oral carcinogenesis model may help to identify important genetic markers for the early detection and progression of head and neck cancer.<\/jats:p><\/jats:sec>","DOI":"10.1177\/019459980112400612","type":"journal-article","created":{"date-parts":[[2017,7,13]],"date-time":"2017-07-13T14:56:24Z","timestamp":1499957784000},"page":"663-668","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Identification of Differentially Expressed Genes in Human Papillomavirus Type\u201016 Infected Oral Cancer Cells"],"prefix":"10.1002","volume":"124","author":[{"given":"Jimmy J.","family":"Brown","sequence":"first","affiliation":[{"name":"Department of Otolaryngology\u2014Head and Neck Surgery Charles R. Drew University of Medicine and Science Los Angeles California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min","family":"Qin","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery Charles R. Drew University of Medicine and Science Los Angeles California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lorraine","family":"William\u2010Smith","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2014Head and Neck Surgery Charles R. Drew University of Medicine and Science Los Angeles California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joan F.","family":"Coker","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2014Head and Neck Surgery Charles R. 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