{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T11:30:35Z","timestamp":1777894235569,"version":"3.51.4"},"reference-count":10,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2010,12,31]],"date-time":"2010-12-31T00:00:00Z","timestamp":1293753600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["aao-hnsfjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Otolaryngol.--head neck surg."],"published-print":{"date-parts":[[2011,1]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>Detection of beta\u20102 transferrin in rhinorrhea fluid is a sensitive and specific method for the diagnosis of a cerebrospinal fluid (CSF) leak. Patients may be asked to collect this fluid at home to obtain an adequate volume for detection, and thus the age and storage conditions of these specimens may be variable upon analysis. The purpose of this study is to understand how age, storage temperature, and exposure to mucus affect the ability to detect beta\u20102 transferrin in CSF.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design<\/jats:title><jats:p>Case series with planned data collection.<\/jats:p><\/jats:sec><jats:sec><jats:title>Setting<\/jats:title><jats:p>Tertiary care university hospital.<\/jats:p><\/jats:sec><jats:sec><jats:title>Subjects and Methods<\/jats:title><jats:p>This study consists of 6 patients undergoing endoscopic CSF leak repair. CSF was collected directly from a lumbar drain (n = 4) or from nasal drainage (n = 2). Specimens were stored at 4\u00b0C (n = 3) or room temperature (n = 3). Samples were tested for the presence of beta\u20102 transferrin for up to 7\u00a0days using standard immunofixation electrophoresis techniques.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Beta\u20102 transferrin was detected in all specimens through day 7 regardless of storage temperature or collection site (95% exact binomial confidence interval of 0%\u201046%).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Beta\u20102 transferrin remains detectable in extracorporeal CSF for up to 7\u00a0days regardless of storage at room temperature or exposure to nasal mucus. Negative detection in patient specimens up to a week old is therefore not likely to be caused by protein degradation.<\/jats:p><\/jats:sec>","DOI":"10.1177\/0194599810390887","type":"journal-article","created":{"date-parts":[[2010,12,31]],"date-time":"2010-12-31T13:38:02Z","timestamp":1293802682000},"page":"101-103","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Preliminary Study on the Stability of Beta\u20102 Transferrin in Extracorporeal Cerebrospinal Fluid"],"prefix":"10.1002","volume":"144","author":[{"given":"Benjamin S.","family":"Bleier","sequence":"first","affiliation":[{"name":"Massachusetts Eye and Ear Infirmary Harvard Medical School Boston Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Indranil","family":"Debnath","sequence":"additional","affiliation":[{"name":"Washington University School of Medicine St Louis Missouri USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brendan P.","family":"O\u2019Connell","sequence":"additional","affiliation":[{"name":"Medical University of South Carolina Charleston South Carolina USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"III","given":"W. Alexander","family":"Vandergrift","sequence":"additional","affiliation":[{"name":"Medical University of South Carolina Charleston South Carolina USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James N.","family":"Palmer","sequence":"additional","affiliation":[{"name":"Hospital of the University of Pennsylvania Philadelphia Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rodney J.","family":"Schlosser","sequence":"additional","affiliation":[{"name":"Medical University of South Carolina Charleston South Carolina USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,12,31]]},"reference":[{"key":"e_1_2_8_2_2","first-page":"E397","article-title":"Cerebrospinal fluid rhinorrhoea: diagnosis and management","volume":"12","author":"Abuabara A","year":"2007","journal-title":"Med Oral Patol Oral Cir Bucal"},{"key":"e_1_2_8_3_2","first-page":"169","article-title":"The diagnosis of CSF fistulae on the basis of detection of beta 2\u2010transferrin by polyacrylamide gel electrophoresis and immunoblotting","volume":"27","author":"Reisinger PW","year":"1989","journal-title":"J Clin Chem Clin Biochem"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-2007-998649"},{"key":"e_1_2_8_5_2","doi-asserted-by":"publisher","DOI":"10.1001\/archotol.130.10.1178"},{"key":"e_1_2_8_6_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-0028-1112229"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.1001\/archotol.1979.00790200009002"},{"key":"e_1_2_8_8_2","doi-asserted-by":"publisher","DOI":"10.3171\/jns.1992.77.5.0737"},{"key":"e_1_2_8_9_2","doi-asserted-by":"publisher","DOI":"10.3109\/00016487909126434"},{"key":"e_1_2_8_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/48.6.939"},{"key":"e_1_2_8_11_2","first-page":"169","article-title":"The diagnosis of CSF fistulae on the basis of detection of beta 2\u2010transferrin by polyacrylamide gel electrophoresis and immunoblotting","volume":"27","author":"Reisinger PW","year":"1989","journal-title":"J Clin Chem Clin Biochem"}],"container-title":["Otolaryngology\u2013Head and Neck Surgery"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1177\/0194599810390887","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1177\/0194599810390887","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/aao-hnsfjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1177\/0194599810390887","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T12:49:24Z","timestamp":1760964564000},"score":1,"resource":{"primary":{"URL":"https:\/\/aao-hnsfjournals.onlinelibrary.wiley.com\/doi\/10.1177\/0194599810390887"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,12,31]]},"references-count":10,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2011,1]]}},"alternative-id":["10.1177\/0194599810390887"],"URL":"https:\/\/doi.org\/10.1177\/0194599810390887","archive":["Portico"],"relation":{},"ISSN":["0194-5998","1097-6817"],"issn-type":[{"value":"0194-5998","type":"print"},{"value":"1097-6817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,12,31]]},"assertion":[{"value":"2010-08-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-09-17","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-12-31","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}