{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T01:12:28Z","timestamp":1785892348217,"version":"3.56.0"},"reference-count":17,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2011,2,18]],"date-time":"2011-02-18T00:00:00Z","timestamp":1297987200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["aao-hnsfjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Otolaryngol.--head neck surg."],"published-print":{"date-parts":[[2011,6]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>To determine the contribution of B\u2010 and T\u2010cell\u2013mediated inflammation in a murine airway granulation model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design<\/jats:title><jats:p>Pilot study in a modified murine model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Setting<\/jats:title><jats:p>Philadelphia VA Medical Center Research Building.<\/jats:p><\/jats:sec><jats:sec><jats:title>Subjects and Methods<\/jats:title><jats:p>Laryngotracheal complexes (LTCs) from 54 donor C57BL\/6\u00a0mice were harvested and divided into 3\u00a0groups: (1) uninjured, (2) mechanically injured using a wire brush, and (3) chemically injured using hydrochloric acid. One donor LTC from each group was placed in deep dorsal subcutaneous pockets of either severe combined immunodeficiency (SCID)\u2013 or C57BL\u2010recipient mice, for a total of 3 transplanted tracheas per recipient mouse. After 3 weeks, the transplanted LTCs were harvested from both C57BL\u2010 and SCID\u2010recipient mice. Tissues were fixed, sectioned, and stained with hematoxylin and eosin. Representative slides were reviewed by a blinded pathologist to determine the formation of granulation tissue and graded as to the degree of formation of granulation tissue.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Despite significant granulation formation in C57BL\u2010recipient mice, direct airway injury did not induce the formation of granulation tissue under the disrupted epithelium of airway mucosa in SCID mice 3 weeks after injury.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The data indicate that the immune response that results in the formation of granulation tissue is mediated by circulating B\u2010 and\/or T\u2010cell processes rather than resident airway immune cells. Further studies focusing on cellular adaptive immune processes in response to airway injury may provide a novel treatment modality for subglottic stenosis.<\/jats:p><\/jats:sec>","DOI":"10.1177\/0194599810397750","type":"journal-article","created":{"date-parts":[[2011,2,18]],"date-time":"2011-02-18T23:50:53Z","timestamp":1298073053000},"page":"927-933","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["Cellular Adaptive Inflammation Mediates Airway Granulation in a Murine Model of Subglottic Stenosis"],"prefix":"10.1002","volume":"144","author":[{"given":"Ankona","family":"Ghosh","sequence":"first","affiliation":[{"name":"Department of Otorhinolaryngology: Head and Neck Surgery University of Pennsylvania Philadelphia Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nora","family":"Malaisrie","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology: Head and 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