{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T00:39:33Z","timestamp":1761007173806,"version":"build-2065373602"},"reference-count":30,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2011,3,4]],"date-time":"2011-03-04T00:00:00Z","timestamp":1299196800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["aao-hnsfjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Otolaryngol.--head neck surg."],"published-print":{"date-parts":[[2011,9]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>Determine the toxicity and efficacy of a novel antiprotease topical irrigation, polyhydrated ionogen (PHI) \u00b1 MgBr<jats:sub>2<\/jats:sub>, in improving sinonasal remucosalization following surgery.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design<\/jats:title><jats:p>Blinded, randomized controlled study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Setting<\/jats:title><jats:p>Academic.<\/jats:p><\/jats:sec><jats:sec><jats:title>Subjects and Methods<\/jats:title><jats:p>Ciliary beat frequency (CBF) of murine nasal septal explants was continuously recorded before and after addition of PHI solution to asses for ciliotoxicity. To evaluate for efficacy in remucosalization, 9\u00a0New Zealand white rabbits underwent bilateral medial\u2010wall maxillary mucosal stripping followed by placement of an indwelling irrigation catheter. In a randomized fashion one side received 3\u00a0mL of normal saline (NS) daily, whereas the contralateral side received PHI \u00b1 MgBr<jats:sub>2<\/jats:sub>. Following a 14\u2010day therapeutic trial, remucosalization was assessed by hematoxylin and eosin staining and immunohistochemistry for \u03b2\u2010tubulin, a marker of cilia. A semiquantitative grading of ciliated remucosalization was applied with a chi\u2010square test to compare the saline with the PHI \u00b1 MgBr<jats:sub>2<\/jats:sub> treatment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Safety evaluation of the PHI solutions demonstrated no evidence of ciliotoxicity. Histologic semiquantitative analysis of maxillary sinus remucosalization demonstrated significantly more ciliated epithelium (&gt;60%) in the majority of PHI (n = 4) and PHI with MgBr<jats:sub>2<\/jats:sub> (n = 5) treatment compared with the saline treatment (&lt;30%) (n = 9). This was confirmed with immunohistochemical staining for type IV \u03b2\u2010tubulin a marker of respiratory cilia.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Success of functional endoscopic sinus surgery depends on restoration of normal mucociliary clearance. Topical PHI application has previously been demonstrated to significantly increase dermal wound healing. PHI solution is not ciliotoxic, and daily topical PHI or PHI MgBr<jats:sub>2<\/jats:sub> irrigation enhances ciliated remucosalization compared with saline.<\/jats:p><\/jats:sec>","DOI":"10.1177\/0194599811399558","type":"journal-article","created":{"date-parts":[[2011,3,4]],"date-time":"2011-03-04T23:04:19Z","timestamp":1299279859000},"page":"482-488","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Murine Ciliotoxicity and Rabbit Sinus Mucosal Healing by Polyhydrated Ionogen"],"prefix":"10.1002","volume":"145","author":[{"given":"David","family":"DePoortere","sequence":"first","affiliation":[{"name":"University of Pennsylvania School of Medicine Department of Otorhinolaryngology Philadelphia Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer M.","family":"Kofonow","sequence":"additional","affiliation":[{"name":"University of Pennsylvania School of Medicine Department of 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