{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T00:40:24Z","timestamp":1761007224793,"version":"build-2065373602"},"reference-count":24,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2011,3,31]],"date-time":"2011-03-31T00:00:00Z","timestamp":1301529600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["aao-hnsfjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Otolaryngol.--head neck surg."],"published-print":{"date-parts":[[2011,7]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>To determine whether variations in gene expression exist at multiple subsites along the sinonasal tract in patients with chronic sinusitis with polyps and in healthy controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design<\/jats:title><jats:p>Prospective, controlled study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Setting<\/jats:title><jats:p>Academic medical center.<\/jats:p><\/jats:sec><jats:sec><jats:title>Subjects and Methods<\/jats:title><jats:p>Tissue expression levels of 5 genes, previously found to be characteristic of ethmoid polyps, were measured using real\u2010time quantitative polymerase chain reaction in 100 sinonasal tissue samples. Specimens harvested from 5 regions\u2014the ethmoid sinus, septum, inferior turbinate, middle turbinate, and lateral nasal wall\u2014in 10 patients with chronic sinusitis and ethmoid polyps were compared to tissue from similar regions in 10 control patients without sinusitis. Western blot analysis was performed to validate differential gene expression at the protein level.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Gene expression levels of ethmoid polyps differed significantly from those of healthy ethmoid mucosa, as well as tissue from 4 surrounding anatomical sites in both patients with chronic sinusitis and controls. Alterations specific to the polyp tissue included downregulated genes, prolactin\u2010induced protein (fold change 377.2 \u00b1 169.0, <jats:italic>P<\/jats:italic> &lt;. 0001), and zinc \u03b12\u2010glycoprotein (fold change 72.1 \u00b1 26.5, <jats:italic>P<\/jats:italic> &lt;. 0001), as well as upregulated genes, met proto\u2010oncogene (fold change 2.5 \u00b1 0.7, <jats:italic>P<\/jats:italic> =. 029), and periostin (fold change 7.5 \u00b1 3.4, <jats:italic>P<\/jats:italic> =. 003). No significant differences in gene expression was found for neurabin 2 (fold change 1.0, <jats:italic>P<\/jats:italic> =. 99).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The transcriptional pattern of ethmoid polyps appears to be unique compared with other subsites in the sinonasal cavity of patients with chronic sinusitis. Care must be taken when collecting specimens for molecular studies of the sinonasal tract to differentiate polyp from nonpolyp tissue in chronic sinusitis.<\/jats:p><\/jats:sec>","DOI":"10.1177\/0194599811402030","type":"journal-article","created":{"date-parts":[[2011,4,1]],"date-time":"2011-04-01T00:13:42Z","timestamp":1301616822000},"page":"171-175","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Topographic Gene Expression in the Sinonasal Cavity of Patients with Chronic Sinusitis with Polyps"],"prefix":"10.1002","volume":"145","author":[{"given":"Michael P.","family":"Platt","sequence":"first","affiliation":[{"name":"Department of Otology and Laryngology Harvard Medical School Boston Massachusetts USA"},{"name":"Department of Otolaryngology\u2013Head and Neck Surgery Boston University Boston Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zachary 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