{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T22:27:33Z","timestamp":1791066453693,"version":"4.1.0"},"reference-count":18,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2011,3,31]],"date-time":"2011-03-31T00:00:00Z","timestamp":1301529600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["aao-hnsfjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Otolaryngol.--head neck surg."],"published-print":{"date-parts":[[2011,8]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>The aim of this study was to determine the prevalence of human papillomavirus (HPV) in 80 tumor\u2010free tonsils from healthy children and adults using a sensitive E6\/E7 type\u2010specific polymerase chain reaction (PCR).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Study Design<\/jats:title>\n                    <jats:p>Cross\u2010sectional study.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Setting<\/jats:title>\n                    <jats:p>Ear, nose, and throat department, university hospital.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Subjects and Methods<\/jats:title>\n                    <jats:p>Paraffin\u2010embedded tissues from tumor\u2010free tonsils (TFTs) were evaluated for HPV DNA using GP5+\/6+ consensus PCR and subsequent genotyping using E6\/E7 type\u2010specific PCR for HPV types 6, 11, 16, 18, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, and 68. The immunohistochemical expression of p16 and p53 was also assessed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>In 80 TFTs, the authors identified 10 (12.5%) that tested positive for the following high\u2010risk HPV types: HPV 16 (8 cases), 18 (1 case), and 31 (1 case). Twelve patients (15%) tested positive for HPV infection using the GP5+\/GP6+ consensus primers but were negative using quantitative PCR. These patients were considered infected with low\u2010risk HPV types. Fifty\u2010eight TFTs (72.5%) tested negative for both GP5+\/GP6+ and type\u2010specific HPV PCR analysis (HPV negative). Among patients infected with HPV, the authors observed a slight increase in frequency with age.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>In TFTs, oncogenic and nononcogenic HPVs were present at a relatively high frequency in children and adults. The presence of high\u2010risk HPV DNA in young children supports the horizontal transmission hypothesis and argues in favor of HPV vaccination at birth.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/0194599811402944","type":"journal-article","created":{"date-parts":[[2011,4,1]],"date-time":"2011-04-01T00:13:42Z","timestamp":1301616822000},"page":"230-235","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["High Prevalence of High\u2010Risk Human Papillomavirus in Palatine Tonsils from Healthy Children and Adults"],"prefix":"10.1002","volume":"145","author":[{"given":"Ana\u00eblle","family":"Duray","sequence":"first","affiliation":[{"name":"Laboratory of Anatomy Faculty of Medicine and Pharmacy University of Mons Mons Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G\u00e9raldine","family":"Descamps","sequence":"additional","affiliation":[{"name":"Laboratory of Anatomy Faculty of 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