{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T10:57:43Z","timestamp":1784804263591,"version":"3.55.0"},"reference-count":58,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2011,9,23]],"date-time":"2011-09-23T00:00:00Z","timestamp":1316736000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["aao-hnsfjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Otolaryngol.--head neck surg."],"published-print":{"date-parts":[[2011,12]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>The degeneration of hair cells and spiral ganglion neurons (SGNs) is an important pathologic process in the development of sensorineural hearing loss. In a murine model, predictable and reproducible damage to SGNs occurs through the application of ouabain to the round window. Recent evidence has shown that the chemokine stromal cell\u2013derived factor\u20101 (SDF\u20101) is a potent chemoattractant of hematopoietic stem cells (HSCs) and provides trophic support to injured tissues during development and maturation. The hypothesis for the current study is that expression of SDF\u20101 plays an important role in protecting SGNs and preventing further degeneration in the setting of cochlear injury.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design<\/jats:title><jats:p>Prospective, controlled.<\/jats:p><\/jats:sec><jats:sec><jats:title>Setting<\/jats:title><jats:p>Academic research laboratory.<\/jats:p><\/jats:sec><jats:sec><jats:title>Subject and Methods<\/jats:title><jats:p>Auditory brainstem response (ABR) and the expression of SDF\u20101\u00a0mRNA and protein were examined 1, 3, 7, 14, and 30\u00a0days after application of ouabain in 35 adult mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Following ouabain application, real\u2010time reverse\u2010transcription polymerase chain reaction for SDF demonstrates increased mRNA expression following ouabain injury in nontransplanted mice. A significant increase in SDF protein expression was also observed using immunolabeling techniques and Western blot analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>SDF\u20101 expression is increased in the auditory nerve following cochlear injury. Further knowledge about the cochlear microenvironment, including SDF\u20101, is critical to maximizing HSC engraftment in the injured cochlea and providing a therapeutic option for sensorineural hearing loss.<\/jats:p><\/jats:sec>","DOI":"10.1177\/0194599811416778","type":"journal-article","created":{"date-parts":[[2011,9,24]],"date-time":"2011-09-24T05:02:47Z","timestamp":1316840567000},"page":"1007-1015","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Role of Stromal Cell\u2010Derived Factor\u20101 Expression in the Injured Mouse Auditory Nerve"],"prefix":"10.1002","volume":"145","author":[{"given":"Lauren A.","family":"Kilpatrick","sequence":"first","affiliation":[{"name":"Department of Otolaryngology\u2014Head and Neck Surgery Medical University of South Carolina Charleston South Carolina 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