{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T05:24:28Z","timestamp":1791177868691,"version":"4.1.0"},"reference-count":29,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2014,8,11]],"date-time":"2014-08-11T00:00:00Z","timestamp":1407715200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["aao-hnsfjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Otolaryngol.--head neck surg."],"published-print":{"date-parts":[[2014,10]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>Examine prophylactic effects of dexamethasone (Dex) in retrocochlear auditory centers in a noise\u2010induced hearing loss (NIHL) mouse model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design<\/jats:title><jats:p>Prospective animal study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Setting<\/jats:title><jats:p>Academic research center.<\/jats:p><\/jats:sec><jats:sec><jats:title>Subjects and Methods<\/jats:title><jats:p>Thirty\u2010two mice were divided into control, untreated, saline (2 and 10 \u00b5L), and Dex (2 and 10 \u00b5L) groups. Dex was applied intratympanically (IT) prior to 110 to 120\u00a0dB noise over 6 hours. Auditory brainstem response (ABR) and distortion product otoacoustic emission (DPOAE) were performed at 1\u00a0day, 1 week, 1\u00a0month, and 2\u00a0months. Retrocochlear neuronal cells were labeled with FluoroGold and counted. Hair cells of the organ of Corti were labeled with fluorescein isothiocyanate\u2010conjugated phalloidin and counted.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Auditory brainstem response thresholds of untreated NIHL, 2 and 10 \u00b5L IT saline, and 2 and 10 \u00b5L IT Dex were 21.7 \u00b1 2.9\u00a0dB, 20 \u00b1 0\u00a0dB, 20 \u00b1 5\u00a0dB, 18.3 \u00b1 2.9\u00a0dB, and 18.3 \u00b1 2.9\u00a0dB, respectively. At 1\u2010day post NIHL, all groups demonstrated profound hearing loss. At 2 weeks, 2 and 10 \u00b5L Dex thresholds improved to 47.5 \u00b1 3.5\u00a0dB and 48.8 \u00b1 18.9\u00a0dB, respectively, whereas the untreated and saline groups remained unchanged. Mean cell counts in the cochlear nucleus (CN), superior olivary complex (SOC), and lateral lemniscus (LL) of control mice were 1483 \u00b1 190, 2807 \u00b1 67, and 112 \u00b1 20, respectively. After acoustic trauma, the untreated, saline, and 2 \u00b5L Dex groups yielded decreased neuronal counts in the SOC. In contrast, the 10 \u00b5L Dex group had 1883 \u00b1 186 (CN), 2774 \u00b1 182 (SOC), and 166 \u00b1 18 (LL). There was sporadic hair cell loss for all traumatized groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our NIHL mouse model demonstrated dose\u2010dependent Dex pretreatment otoprotection against NIHL with preservation of retrocochlear auditory neurons.<\/jats:p><\/jats:sec>","DOI":"10.1177\/0194599814545771","type":"journal-article","created":{"date-parts":[[2014,8,12]],"date-time":"2014-08-12T02:59:32Z","timestamp":1407812372000},"page":"667-674","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Dexamethasone's Effect in the Retrocochlear Auditory Centers of a Noise\u2010Induced Hearing Loss Mouse Model"],"prefix":"10.1002","volume":"151","author":[{"given":"Leon","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Otolaryngology\u2013Head and Neck Surgery New York Eye and Ear Infirmary New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clare","family":"Dean","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2013Head and Neck Surgery New York Eye and Ear Infirmary New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michele","family":"Gandolfi","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2013Head and Neck Surgery New York Eye and Ear Infirmary New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edmund","family":"Nahm","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2013Head and Neck Surgery New York Eye and Ear Infirmary New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda","family":"Mattiace","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Anatomy New York Medical College Valhalla New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana H.","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2013Head and Neck Surgery New York Eye and Ear Infirmary New York New York USA"},{"name":"Department of Cell Biology and Anatomy New York Medical College Valhalla New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,8,11]]},"reference":[{"key":"e_1_2_8_2_1","unstructured":"National Institute for Occupational Safety and Health.Work related hearing loss. DHHS (NIOSH) Publication No. 2001\u2010103.http:\/\/www.cdc.gov\/niosh\/docs\/2001\u2010103\/. Accessed July 21 2014."},{"key":"e_1_2_8_3_1","volume-title":"Noise and Military Service. Implications for Hearing Loss and Tinnitus","author":"National Research Council","year":"2006"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-5955(83)90044-8"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00456329"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-5955(87)90036-0"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1080\/00016480120524"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.aud.0000191942.36672.f3"},{"key":"e_1_2_8_9_1","first-page":"159","article-title":"Reactive oxygen species, apoptosis, and necrosis in noise\u2010exposed cochleas of chinchillas","volume":"22","author":"Nicotera TM","year":"1999","journal-title":"Abs Assoc Res Otolaryngol"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heares.2006.10.006"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0194-5998(96)70133-X"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1177\/000348940211101213"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-5955(94)00171-L"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903410209"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2012.10.032"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-5955(00)00218-5"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.20795"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heares.2004.06.003"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.23185"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heares.2011.06.003"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1089\/neu.2009.1246"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1159\/000027764"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.23093"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-5955(96)00173-6"},{"key":"e_1_2_8_25_1","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/S0026-895X(25)09102-3","article-title":"Delayed increase of Ca2+ influx elicited by glutamate: role in neuronal death","volume":"35","author":"Manev H","year":"1989","journal-title":"Molecular Pharmacol"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1097\/MAO.0b013e31825e7e12"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.3109\/00206099209081653"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-5955(89)90013-0"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heares.2006.10.006"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddq028"}],"container-title":["Otolaryngology\u2013Head and Neck Surgery"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1177\/0194599814545771","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1177\/0194599814545771","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/aao-hnsfjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1177\/0194599814545771","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T08:51:09Z","timestamp":1760950269000},"score":1,"resource":{"primary":{"URL":"https:\/\/aao-hnsfjournals.onlinelibrary.wiley.com\/doi\/10.1177\/0194599814545771"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,8,11]]},"references-count":29,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2014,10]]}},"alternative-id":["10.1177\/0194599814545771"],"URL":"https:\/\/doi.org\/10.1177\/0194599814545771","archive":["Portico"],"relation":{},"ISSN":["0194-5998","1097-6817"],"issn-type":[{"value":"0194-5998","type":"print"},{"value":"1097-6817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,8,11]]},"assertion":[{"value":"2013-10-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-07-11","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-08-11","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}