{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T00:32:55Z","timestamp":1761006775837,"version":"build-2065373602"},"reference-count":30,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2015,6,29]],"date-time":"2015-06-29T00:00:00Z","timestamp":1435536000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["aao-hnsfjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Otolaryngol.--head neck surg."],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>To explore the role of antiapoptotic and apoptotic processes in the development of cholesteatoma by investigating the expression of an antiapoptotic (c\u2010FLIP) and apoptotic (p53) protein relative to the expression of a proliferation marker (Ki\u201067).<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design<\/jats:title><jats:p>Basic science study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Setting<\/jats:title><jats:p>Tertiary referral center.<\/jats:p><\/jats:sec><jats:sec><jats:title>Subjects and Methods<\/jats:title><jats:p>An immunohistochemical investigation was performed on 35 cholesteatoma specimens (21 acquired, 14 congenital) and 10\u00a0normal retroauricular skins to evaluate the expression of c\u2010FLIP, p53, and Ki\u201067. The expression rate of each marker was measured to assess the difference between retroauricular skin and cholesteatoma, as well as between congenital and acquired cholesteatoma.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>c\u2010FLIP expression was significantly higher in the cholesteatoma specimens than in retroauricular skin (<jats:italic>P<\/jats:italic> &lt;. 05), while the expression of p53 did not significantly differ between the two. Ki\u201067 expression in cholesteatoma was significantly higher than in retroauricular skin (<jats:italic>P<\/jats:italic> &lt;. 001). The c\u2010FLIP expression rate was positively correlated with that of Ki\u201067 (<jats:italic>r<\/jats:italic> = 0.47, <jats:italic>P<\/jats:italic> =. 001), and there was no significant correlation between the expression level of p53 and that of Ki\u201067 (<jats:italic>r<\/jats:italic> = 0.152, <jats:italic>P<\/jats:italic> =. 319). In addition, no differences in c\u2010FLIP, p53, and Ki\u201067 expression rates were evident between congenital and acquired cholesteatoma.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The upregulation of c\u2010FLIP together with unchanged p53 suggests an altered equilibrium between apoptosis and antiapoptosis, favoring antiapoptosis, and may play a role in the pathogenesis of cholesteatoma.<\/jats:p><\/jats:sec>","DOI":"10.1177\/0194599815591810","type":"journal-article","created":{"date-parts":[[2015,6,29]],"date-time":"2015-06-29T23:05:30Z","timestamp":1435619130000},"page":"1024-1030","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Expression of Apoptotic vs Antiapoptotic Proteins in Middle Ear Cholesteatoma"],"prefix":"10.1002","volume":"153","author":[{"given":"Jae Ho","family":"Chung","sequence":"first","affiliation":[{"name":"Departments of Otolaryngology\u2013Head and Neck Surgery School of Medicine Hanyang University Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seung Hwan","family":"Lee","sequence":"additional","affiliation":[{"name":"Departments of Otolaryngology\u2013Head and Neck Surgery School of Medicine Hanyang University Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chul Won","family":"Park","sequence":"additional","affiliation":[{"name":"Departments of Otolaryngology\u2013Head and Neck Surgery School of Medicine Hanyang University Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyung Rae","family":"Kim","sequence":"additional","affiliation":[{"name":"Departments of Otolaryngology\u2013Head and Neck Surgery School of Medicine Hanyang University Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyung","family":"Tae","sequence":"additional","affiliation":[{"name":"Departments of Otolaryngology\u2013Head and Neck Surgery School of Medicine Hanyang University Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sung\u2010Ho","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology Konkuk University School of Medicine Chungju Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Young Ha","family":"Oh","sequence":"additional","affiliation":[{"name":"Departments of Pathology School of Medicine Hanyang University Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ju Yeon","family":"Pyo","sequence":"additional","affiliation":[{"name":"Departments of Pathology School of Medicine Hanyang University Seoul 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