{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T11:16:44Z","timestamp":1792149404255,"version":"4.4.0"},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2015,9,29]],"date-time":"2015-09-29T00:00:00Z","timestamp":1443484800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["aao-hnsfjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Otolaryngol.--head neck surg."],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>To evaluate whether the calcium\u2010channel blocker diltiazem has protective effects against cisplatin\u2010induced ototoxicity in a mouse model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design<\/jats:title><jats:p>Original basic science in vivo investigation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Setting<\/jats:title><jats:p>Academic setting: Otolaryngology\u2013Head and Neck Surgery laboratory at University of Connecticut Health Center.<\/jats:p><\/jats:sec><jats:sec><jats:title>Subjects<\/jats:title><jats:p>Thirty\u2010nine female CBA\/J mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Pure tone\u2013 or click\u2010evoked auditory brainstem responses (ABRs) were recorded in CBA\/J mice to determine auditory thresholds. All mice had baseline ABRs recorded. They were then given a single cisplatin bolus (14\u00a0mg\/kg), followed by 5 consecutive days of intratympanic diltiazem or saline control. Follow\u2010up thresholds were recorded on days 7, 14, and 21 postcisplatin. Tone\u2010evoked ABRs evaluated the otoprotective effect of 2\u2010mg\/kg diltiazem in 9\u00a0mice, and dose effect was examined in response to click\u2010evoked ABR with 2\u2010 or 4\u2010mg\/kg diltiazem in 2\u00a0groups of 15\u00a0mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Saline\u2010treated ears had significantly elevated tone\u2010evoked auditory thresholds when compared with diltiazem\u2010treated ears (<jats:italic>P<\/jats:italic> =. 038) on day 7 postcisplatin only. Click\u2010evoked ABR thresholds were significantly elevated in saline\u2010treated ears versus diltiazem\u2010treated ears for the 2\u2010mg\/kg group (<jats:italic>P<\/jats:italic> =. 001) and 4\u2010mg\/kg group (<jats:italic>P<\/jats:italic> =. 011) on days 7, 14, and 21 postcisplatin.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Intratympanic diltiazem has significant protective effects against cisplatin ototoxicity at 2 and 4\u00a0mg\/kg. This is the first in vivo study to demonstrate that diltiazem offers a potentially novel therapy for cisplatin\u2010induced ototoxicity.<\/jats:p><\/jats:sec>","DOI":"10.1177\/0194599815606704","type":"journal-article","created":{"date-parts":[[2015,9,29]],"date-time":"2015-09-29T23:13:32Z","timestamp":1443568412000},"page":"144-149","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Cisplatin\u2010Induced Ototoxicity and the Effects of Intratympanic Diltiazem in a Mouse Model"],"prefix":"10.1002","volume":"154","author":[{"given":"James G.","family":"Naples","sequence":"first","affiliation":[{"name":"University of Connecticut Health Center Farmington Connecticut USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kourosh","family":"Parham","sequence":"additional","affiliation":[{"name":"University of Connecticut Health Center Farmington Connecticut 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