{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T23:59:03Z","timestamp":1790380743266,"version":"4.1.0"},"reference-count":15,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,3,22]],"date-time":"2016-03-22T00:00:00Z","timestamp":1458604800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["aao-hnsfjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Otolaryngol.--head neck surg."],"published-print":{"date-parts":[[2016,6]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>To describe the findings on the presbylarynx under scanning electron microscopy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design<\/jats:title><jats:p>Cadaver study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Setting<\/jats:title><jats:p>Universidade Estadual Paulista (Botucatu, S\u00e3o Paulo, Brazil).<\/jats:p><\/jats:sec><jats:sec><jats:title>Subjects and Methods<\/jats:title><jats:p>Sixteen vocal folds were removed during necropsies and distributed into 2 age groups: control (n = 8; aged 30\u201050 years) and elderly (n = 8; aged 75\u201092 years). The right vocal fold was dissected, fixed in glutaraldehyde 2.5%, and prepared for scanning electron microscopy. The thickness of the epithelium was measured using a scandium morphometric digital program.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In the control group, the epithelium had 5 to 7 overlapped cell layers, rare desquamation cells, and little undulation with protruding intercellular junctions. The lamina propria showed a uniform network of collagen and elastic fibers in the superficial layer. A dense network of collagen was identified in the deeper layer. In the elderly group, the epithelium was atrophic (2\u20103 cells), with more desquamation cells and intercellular junctions delimited by deep sulci. The epithelial thickness was lower in elderly than in controls (mean [SD], 221.64 [145.90] \u00b5m vs 41.79 [21.40] \u00b5m, respectively). The lamina propria had a dense and irregular distribution of collagen and elastic fibers in the superficial layer. In the deep layers, the collagen fibers formed a true fibrotic and rigid skeleton.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Scanning electron microscopy identified several changes in the elderly larynx, differentiating it from the controls. These alterations are probably related to the aging process of the vocal folds. However, the exact interpretation of these findings requires additional studies, even to the molecular level, having the fibroblasts as targets.<\/jats:p><\/jats:sec>","DOI":"10.1177\/0194599816637081","type":"journal-article","created":{"date-parts":[[2016,3,22]],"date-time":"2016-03-22T22:24:55Z","timestamp":1458685495000},"page":"1073-1078","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Scanning Electron Microscopy of the Presbylarynx"],"prefix":"10.1002","volume":"154","author":[{"given":"Tatiana Maria","family":"Gon\u00e7alves","sequence":"first","affiliation":[{"name":"Department of Ophthalmology Otorhinolaryngology and Head and Neck Surgery Univ Estadual Paulista Botucatu Medical School (Unesp) Botucatu (SP) Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela Carvalho","family":"dos Santos","sequence":"additional","affiliation":[{"name":"Institute of Bioscience Department of Electron Microscopy Univ Estadual Paulista (Unesp) Botucatu (SP) Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriana Bueno Benito","family":"Pessin","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Otorhinolaryngology and Head and Neck Surgery Univ Estadual Paulista Botucatu Medical School (Unesp) Botucatu (SP) Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Regina Helena Garcia","family":"Martins","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Otorhinolaryngology and Head and Neck Surgery Univ Estadual Paulista Botucatu Medical School (Unesp) Botucatu (SP) Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,3,22]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/MOO.0b013e328166794f"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anl.2009.11.013"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jvoice.2010.10.024"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40520-013-0143-5"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jvoice.2004.09.002"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/000348940211100103"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1177\/000348940311201012"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1177\/000348940911801009"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1159\/000091729"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1177\/000348940010901004"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1080\/000164800750001215"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005537-200006000-00025"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0749-0690(18)30695-5"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12929-015-0167-6"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/ca.21114"}],"container-title":["Otolaryngology\u2013Head and Neck Surgery"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1177\/0194599816637081","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1177\/0194599816637081","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/aao-hnsfjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1177\/0194599816637081","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T13:25:29Z","timestamp":1760966729000},"score":1,"resource":{"primary":{"URL":"https:\/\/aao-hnsfjournals.onlinelibrary.wiley.com\/doi\/10.1177\/0194599816637081"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,3,22]]},"references-count":15,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2016,6]]}},"alternative-id":["10.1177\/0194599816637081"],"URL":"https:\/\/doi.org\/10.1177\/0194599816637081","archive":["Portico"],"relation":{},"ISSN":["0194-5998","1097-6817"],"issn-type":[{"value":"0194-5998","type":"print"},{"value":"1097-6817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,3,22]]},"assertion":[{"value":"2015-09-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-02-12","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-03-22","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}