{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T06:27:44Z","timestamp":1785738464109,"version":"3.56.0"},"reference-count":36,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2016,10,3]],"date-time":"2016-10-03T00:00:00Z","timestamp":1475452800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100007698","name":"University of Florida","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007698","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["aao-hnsfjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Otolaryngol.--head neck surg."],"published-print":{"date-parts":[[2017,2]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>To assess collagen and \u03b1\u2010tubulin levels of mouse tympanic membrane fibroblasts treated with quinolone and aminoglycoside antibiotics at concentrations found in eardrops.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design<\/jats:title><jats:p>Prospective controlled cell culture study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Setting<\/jats:title><jats:p>Academic tertiary medical center.<\/jats:p><\/jats:sec><jats:sec><jats:title>Subjects<\/jats:title><jats:p>Mouse tympanic membrane fibroblasts.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In experiment 1, fibroblasts were treated with the following for 24 or 48 hours: phosphate\u2010buffered saline (negative control), dilute hydrochloric acid (positive control), 0.5% gatifloxacin, or commercially available 0.3% ciprofloxacin, 0.3% ciprofloxacin + 0.1% dexamethasone, 0.3% ofloxacin, 0.5% moxifloxacin, 0.3% gentamicin, or 3.5\u00a0mg\/mL of neomycin + polymyxin B sulfate + hydrocortisone. In experiment 2, cells were treated with the pure form of gatifloxacin, gentamicin, ofloxacin, or ciprofloxacin. Cells were observed with phase\u2010contrast microscope until harvested. Proteins were extracted for Western blotting with antibodies against collagen \u03b11 type I (collagen 1A1) and \u03b1\u2010tubulin, and for densitometry to quantify levels.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Collagen and tubulin levels in fibroblasts treated with ofloxacin, moxifloxacin, gatifloxacin, or gentamicin for 24 hours were not different from the saline control. Fibroblasts treated with neomycin + polymyxin B + hydrocortisone, ciprofloxacin + dexamethasone, or ciprofloxacin for 24 hours had lower collagen 1A1 and \u03b1\u2010tubulin levels (all <jats:italic>P<\/jats:italic> &lt;. 001) than the negative control. After 48 hours, fibroblasts treated with neomycin + polymyxin B sulfate + hydrocortisone, ciprofloxacin + dexamethasone, ciprofloxacin, or moxifloxacin had lower collagen 1A1 (<jats:italic>P<\/jats:italic> \u2264. 007) and \u03b1\u2010tubulin (<jats:italic>P<\/jats:italic> &lt;. 0001; except ciprofloxacin, <jats:italic>P<\/jats:italic> =. 033) as compared with control. In experiment 2, only cells treated with ciprofloxacin had lower collagen 1A1 and \u03b1\u2010tubulin levels and cell viability (all <jats:italic>P<\/jats:italic> &lt;. 0001) than control. Cytotoxicity assay and phase\u2010contrast images mirrored the protein findings.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The adverse impact of topical antibiotic exposure on tympanic membrane collagen and tubulin protein levels is drug specific. This may be important for selection of ototopical therapy.<\/jats:p><\/jats:sec>","DOI":"10.1177\/0194599816672627","type":"journal-article","created":{"date-parts":[[2016,9,27]],"date-time":"2016-09-27T21:48:59Z","timestamp":1475012939000},"page":"341-349","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Collagen and \u03b1\u2010Tubulin of Mouse Tympanic Membrane Fibroblasts Treated with Quinolones and Aminoglycosides"],"prefix":"10.1002","volume":"156","author":[{"given":"Bailey A.","family":"Milne\u2010Davies","sequence":"first","affiliation":[{"name":"Department of Otolaryngology\u2013Head and Neck Surgery University of Florida Gainesville Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick J.","family":"Antonelli","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2013Head and Neck Surgery University of Florida Gainesville Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicklas C.","family":"Orobello","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2013Head and Neck Surgery University of Florida Gainesville Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolyn O.","family":"Dirain","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2013Head and Neck Surgery University of Florida Gainesville Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,10,3]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/0194599813517083"},{"key":"e_1_2_7_3_1","first-page":"CD008512","article-title":"Interventions for the prevention of postoperative ear discharge after insertion of ventilation tubes (grommets) in children","volume":"4","author":"Syed MI","year":"2013","journal-title":"Cochrane Database Syst Rev"},{"key":"e_1_2_7_4_1","unstructured":"FDA approved otic antibiotics.http:\/\/dailymed.nlm.nih.gov\/dailymed\/index.cfm. 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