{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T13:29:55Z","timestamp":1761744595045,"version":"build-2065373602"},"reference-count":32,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,10,3]],"date-time":"2017-10-03T00:00:00Z","timestamp":1506988800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["aao-hnsfjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Otolaryngol.--head neck surg."],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>To determine molecularly the presence of measles virus genetic material in the stapes of patients with otosclerosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design<\/jats:title><jats:p>A cross\u2010sectional study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Setting<\/jats:title><jats:p>A tertiary referral hospital.<\/jats:p><\/jats:sec><jats:sec><jats:title>Subjects and Methods<\/jats:title><jats:p>Genetic material was extracted from the stapes of patients with otosclerosis (n = 93) during the period from March 2011 to April 2012. The presence of viral measles sequences was evaluated by the real\u2010time reverse transcriptase polymerase chain reaction (RT\u2010PCR). The expression of the CD46 gene was determined.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Ninety\u2010three patients were included in the study. No sample was positive for any of 3\u00a0measles virus genes (H, N, and F). Measles virus RNA was not detected in any sample by real\u2010time RT\u2010PCR. CD46 levels were positive in 3.3% (n = 3) and negative in 96.7% (n = 90).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study does not support the theory of measles virus as the cause of otosclerosis. It is necessary to do more research about other causal theories to clarify its etiology and prevention.<\/jats:p><\/jats:sec>","DOI":"10.1177\/0194599817733674","type":"journal-article","created":{"date-parts":[[2017,10,3]],"date-time":"2017-10-03T12:00:51Z","timestamp":1507032051000},"page":"158-162","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Absence of Measles Virus Detection from Stapes of Patients with Otosclerosis"],"prefix":"10.1002","volume":"158","author":[{"given":"Mar\u00eda de Lourdes","family":"Flores\u2010Garc\u00eda","sequence":"first","affiliation":[{"name":"Department of Otorhinolaryngology and Head and Neck Surgery Instituto Nacional de Rehabilitaci\u00f3n Luis Guillermo Ibarra Ibarra Mexico City Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia Adriana","family":"Col\u00edn\u2010Castro","sequence":"additional","affiliation":[{"name":"Infectious Disease Division Instituto Nacional de Rehabilitaci\u00f3n Luis Guillermo Ibarra Ibarra Mexico City Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mario Sabas","family":"Hern\u00e1ndez\u2010Palestina","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology and Head and Neck Surgery Instituto Nacional de Rehabilitaci\u00f3n Luis Guillermo Ibarra Ibarra Mexico City Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberto","family":"S\u00e1nchez\u2010Larios","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology and Head and Neck Surgery Instituto Nacional de Rehabilitaci\u00f3n Luis Guillermo Ibarra Ibarra Mexico City Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rafael","family":"Franco\u2010Cendejas","sequence":"additional","affiliation":[{"name":"Infectious Disease Division Instituto Nacional de Rehabilitaci\u00f3n Luis Guillermo Ibarra Ibarra Mexico City Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,10,3]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00405-008-0790-x"},{"key":"e_1_2_9_3_1","first-page":"25","article-title":"Filamentous structures morphologically similar to viral nucleocapsids in otosclerotic lesions in two patients","volume":"7","author":"McKenna MJ","year":"1986","journal-title":"Am J Otol"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1159\/000276627"},{"volume-title":"Isolation and Identification of Nucleic Acid Sequences from Celloidin Embedded Human Bone Sections Using Polymerase Chain Reaction","year":"1994","author":"McKenna M.","key":"e_1_2_9_5_1"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-83-6-1431"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00405-007-0247-7"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/479482"},{"key":"e_1_2_9_9_1","first-page":"93","article-title":"Measles virus prevalence in otosclerotic foci","volume":"65","author":"Karosi T","year":"2007","journal-title":"Adv Otorhinolaryngol"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/00129492-200407000-00009"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.MLG.0000165462.35495.DF"},{"key":"e_1_2_9_12_1","first-page":"86","article-title":"Measles virus and otosclerosis","volume":"65","author":"Niedermeyer HP","year":"2007","journal-title":"Adv Otorhinolaryngol"},{"key":"e_1_2_9_13_1","first-page":"107","article-title":"Antimeasles immunoglobulin G and virus\u2010neutralizing activity in sera of patients with otosclerosis","volume":"65","author":"Lolov S","year":"2007","journal-title":"Adv Otorhinolaryngol"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.06163-11"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.7.2655-2660.2000"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00894-06"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1136\/mp.55.2.84"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2009.10.011"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2006.236"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.3109\/00016488909138366"},{"key":"e_1_2_9_21_1","first-page":"415","article-title":"Immunohistochemical evidence of measles virus antigens in active otosclerosis","volume":"101","author":"McKenna MJ","year":"1989","journal-title":"Eur Arch Otorhinolaryngol"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2007-998631"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0022215100106693"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.3109\/00016489509139314"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1177\/014556130007900807"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.mlg.0000225928.35838.e5"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00405-007-0253-9"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.mao.0000169304.72519.d2"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.mlg.0000178325.48942.f1"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2014.08.027"},{"key":"e_1_2_9_31_1","first-page":"827","article-title":"Polymerase chain reaction amplification of a measles virus sequence from human temporal bone sections with active otosclerosis","volume":"17","author":"McKenna MJ","year":"1996","journal-title":"Am J Otol"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.4314\/eamj.v82i5.9310"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1097\/00129492-200103000-00023"}],"container-title":["Otolaryngology\u2013Head and Neck Surgery"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1177\/0194599817733674","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1177\/0194599817733674","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/aao-hnsfjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1177\/0194599817733674","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T11:06:23Z","timestamp":1760958383000},"score":1,"resource":{"primary":{"URL":"https:\/\/aao-hnsfjournals.onlinelibrary.wiley.com\/doi\/10.1177\/0194599817733674"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,10,3]]},"references-count":32,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2018,1]]}},"alternative-id":["10.1177\/0194599817733674"],"URL":"https:\/\/doi.org\/10.1177\/0194599817733674","archive":["Portico"],"relation":{},"ISSN":["0194-5998","1097-6817"],"issn-type":[{"type":"print","value":"0194-5998"},{"type":"electronic","value":"1097-6817"}],"subject":[],"published":{"date-parts":[[2017,10,3]]},"assertion":[{"value":"2017-04-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-09-06","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-10-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}