{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,17]],"date-time":"2026-05-17T00:03:23Z","timestamp":1778976203561,"version":"3.51.4"},"reference-count":32,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,1,16]],"date-time":"2018-01-16T00:00:00Z","timestamp":1516060800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01 AR056145"],"award-info":[{"award-number":["R01 AR056145"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["aao-hnsfjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Otolaryngol.--head neck surg."],"published-print":{"date-parts":[[2018,4]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>To assess the potential of infrared fiber\u2010optic spectroscopy to evaluate the compositional properties of human tracheal cartilage.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design<\/jats:title><jats:p>Laboratory\u2010based study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Twenty human cadaveric distal tracheas were harvested (age range 20\u201078 years; 6 females, 14\u00a0males) for compositional analysis. Histologic staining, Fourier transform infrared imaging spectroscopy data on collagen and proteoglycan (PG) content, and near\u2010infrared (NIR) fiber\u2010optic probe spectroscopic data that reflect protein and water content were evaluated. NIR fiber\u2010optic probe data were also obtained from the proximal trachea in 4 human cadavers (age range 51\u201065 years; 2 females, 2\u00a0males) in situ for comparison to distal trachea spectral data.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In the distal trachea cohort, the spectroscopic\u2010determined ratio of PG\/amide I, indicative of the relative amount of PG, was significantly higher in the tissues from the younger group compared to the older group (0.37 \u00b1 0.08 vs 0.32 \u00b1 0.05, <jats:italic>P<\/jats:italic> =. 05). A principal component analysis of the NIR spectral data enabled separation of spectra based on tracheal location, likely due to differences in both protein and water content. The NIR\u2010determined water content based on the 5200\u2010cm<jats:sup>\u22121<\/jats:sup> peak was significantly higher in the distal trachea compared to the proximal trachea (<jats:italic>P<\/jats:italic> &lt;. 001).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Establishment of normative compositional values and further elucidating differences between the segments of trachea will enable more directed research toward appropriate compositional end points in regenerative medicine for tracheal repair.<\/jats:p><\/jats:sec>","DOI":"10.1177\/0194599817752310","type":"journal-article","created":{"date-parts":[[2018,1,16]],"date-time":"2018-01-16T14:50:09Z","timestamp":1516114209000},"page":"688-694","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Compositional Assessment of Human Tracheal Cartilage by Infrared Spectroscopy"],"prefix":"10.1002","volume":"158","author":[{"given":"Gary","family":"Linkov","sequence":"first","affiliation":[{"name":"Department of Otolaryngology\u2013Head &amp; Neck Surgery Lewis Katz School of Medicine at Temple University Philadelphia Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arash","family":"Hanifi","sequence":"additional","affiliation":[{"name":"Tissue Imaging and Spectroscopy Laboratory Department of Bioengineer\u2010ing Temple University Philadelphia Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Farzad","family":"Yousefi","sequence":"additional","affiliation":[{"name":"Tissue Imaging and Spectroscopy Laboratory Department of Bioengineer\u2010ing Temple University Philadelphia Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Derrick","family":"Tint","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2013Head &amp; Neck Surgery Lewis Katz School of Medicine at Temple University Philadelphia Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sudheer","family":"Bolla","sequence":"additional","affiliation":[{"name":"Department of Thoracic Medicine &amp; Surgery Lewis Katz School of Medicine at Temple University Philadelphia Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathanial","family":"Marchetti","sequence":"additional","affiliation":[{"name":"Department of Thoracic Medicine &amp; Surgery Lewis Katz School of Medicine at Temple University Philadelphia Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmed M. 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