{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T12:17:52Z","timestamp":1791980272876,"version":"4.4.0"},"reference-count":13,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,5,15]],"date-time":"2018-05-15T00:00:00Z","timestamp":1526342400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["CA206517"],"award-info":[{"award-number":["CA206517"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["aao-hnsfjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Otolaryngol.--head neck surg."],"published-print":{"date-parts":[[2018,9]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>To identify whether TIM\u20103 expression is present in the mast cell population within nasal polyps and to determine its correlation with clinical severity in patients with chronic rhinosinusitis with nasal polyposis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design<\/jats:title><jats:p>Basic science, translational study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Setting<\/jats:title><jats:p>Nasal polyp tissue collected from patients seen at a tertiary care hospital (2015\u20102016).<\/jats:p><\/jats:sec><jats:sec><jats:title>Subjects and Methods<\/jats:title><jats:p>Nasal polyp tissue obtained during functional endoscopic sinus surgery (n = 24) was enzymatically digested into epithelial and stromal fractions. Viable mast cells expressing TIM\u20103 were identified using flow cytometry for the following: CD45, Live\/Dead, c\u2010kit, Fc\u03b5R1, TIM\u20103. Disease severity was assessed using the Sino\u2010Nasal Outcome Test, Lund\u2010Mackay staging system, Lund\u2010Kennedy staging system, and complete blood counts.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Mast cells were found in both the epithelial and stromal layers of polyps, with a greater %TIM\u20103<jats:sup>+<\/jats:sup> mast cells in the epithelial layer compared with that of the stromal layer (<jats:italic>P<\/jats:italic> =. 001). As the percentage of mast cells increased, there was a comparative worsening in endoscopic severity after comparing pre\u2010 and postoperative LK scores (\u03c1 = \u22120.455, <jats:italic>P<\/jats:italic> =. 029). In a subgroup of patients with concomitant asthma, increased epithelial %TIM\u20103<jats:sup>+<\/jats:sup> mast cells also correlated with worsening endoscopic appearance postoperatively (\u03c1 = 0.866, <jats:italic>P<\/jats:italic> =. 001, n = 11). Oral corticosteroid treatment did not change the viability of mast cells nor their influence on the increased postoperative endoscopic disease severity (\u03c1 = \u22120.544, <jats:italic>P<\/jats:italic> =. 020, n = 18).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Viable mast cells were found to be present in polyps with increased TIM\u20103 expression at the epithelial layer. This suggests that TIM\u20103\u00a0may play a role in chronic inflammation in CRSwNP via mast cell activation.<\/jats:p><\/jats:sec>","DOI":"10.1177\/0194599818774560","type":"journal-article","created":{"date-parts":[[2018,5,15]],"date-time":"2018-05-15T04:49:53Z","timestamp":1526359793000},"page":"581-586","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["TIM\u20103\u2013Expressing Mast Cells Are Present in Chronic Rhinosinusitis with Nasal Polyps"],"prefix":"10.1002","volume":"159","author":[{"given":"Erica","family":"Corredera","sequence":"first","affiliation":[{"name":"University of Pittsburgh School of Medicine Pittsburgh Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Binh L.","family":"Phong","sequence":"additional","affiliation":[{"name":"Department of Immunology University of Pittsburgh Pittsburgh Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John A.","family":"Moore","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2014Head and Neck Surgery Division of Sinonasal Disorders and Allergy UPMC Mercy Pittsburgh Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lawrence P.","family":"Kane","sequence":"additional","affiliation":[{"name":"Department of Immunology University of Pittsburgh Pittsburgh Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stella E.","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2014Head and Neck Surgery Division of Sinonasal Disorders and Allergy UPMC Mercy Pittsburgh Pennsylvania 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