{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T11:40:46Z","timestamp":1768736446396,"version":"3.49.0"},"reference-count":46,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,10,2]],"date-time":"2018-10-02T00:00:00Z","timestamp":1538438400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000070","name":"National Institute of Biomedical Imaging and Bioengineering","doi-asserted-by":"publisher","award":["P41 EB002520"],"award-info":[{"award-number":["P41 EB002520"]}],"id":[{"id":"10.13039\/100000070","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["aao-hnsfjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Otolaryngol.--head neck surg."],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>To assess the efficacy of acellular bilayer silk fibroin (BLSF) grafts to repair full\u2010thickness tracheal defects and to compare the performance with conventional porcine small intestinal submucosa (SIS) implants.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design<\/jats:title><jats:p>A prospective controlled animal trial in a rat model of onlay tracheoplasty.<\/jats:p><\/jats:sec><jats:sec><jats:title>Setting<\/jats:title><jats:p>Pediatric medical center.<\/jats:p><\/jats:sec><jats:sec><jats:title>Subjects and Methods<\/jats:title><jats:p>Tracheal reconstruction of adult Sprague\u2010Dawley rats was performed with BLSF (n = 38) or SIS (n = 32) matrices for up to 3\u00a0months of implantation. Functional evaluations of repaired conduits as well as histologic, immunohistochemical, and histomorphometric analyses of neotissues were assessed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Prior to scheduled euthanasia, survival rates of rats receiving BLSF or SIS grafts were \u226594%, with no clinical signs of airway obstruction observed over the course of the study. Micro\u2013computed tomography analysis revealed that the mean percentage of stenosis was &lt;20% in both implant groups. BLSF and SIS grafts supported formation of pseudostratified ciliated columnar epithelium by 1 week postoperatively; however, each matrix failed to promote de novo chondrogenesis by 3\u00a0months following repair.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>BLSF scaffolds can be used for reconstruction of rat tracheal patch defects with functional outcomes comparable to those of SIS matrices.<\/jats:p><\/jats:sec>","DOI":"10.1177\/0194599818802267","type":"journal-article","created":{"date-parts":[[2018,10,2]],"date-time":"2018-10-02T04:18:21Z","timestamp":1538453901000},"page":"310-319","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Evaluation of Acellular Bilayer Silk Fibroin Grafts for Onlay Tracheoplasty in a Rat Defect Model"],"prefix":"10.1002","volume":"160","author":[{"given":"Cinthia","family":"Galvez Alegria","sequence":"first","affiliation":[{"name":"Urological Diseases 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