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Protein, molecular, and flow cytometric techniques were applied in vitro to assess the phenotype and functional response of disease fibroblasts to IL\u201017A.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Mechanistically, IL\u201017A drives iSGS scar fibroblast proliferation (<jats:italic>P<\/jats:italic> &lt;. 01), synergizes with transforming growth factor \u00df1 to promote extracellular matrix production (collagen and fibronectin; <jats:italic>P<\/jats:italic> =. 04), and directly stimulates scar fibroblasts to produce chemokines (chemokine ligand 2) and cytokines (IL\u20106 and granulocyte\u2010macrophage colony\u2010stimulating factor) critical to the recruitment and differentiation of myeloid cells (<jats:italic>P<\/jats:italic> &lt;. 01). Glucocorticoids abrogated IL\u201017A\u2010dependent iSGS scar fibroblast production of granulocyte\u2010macrophage colony\u2010stimulating factor (<jats:italic>P<\/jats:italic> =. 02).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>IL\u201017A directly drives iSGS scar fibroblast proliferation, synergizes with transforming growth factor \u00df1 to promote extracellular matrix production, and amplifies local inflammatory signaling. Glucocorticoids appear to partially abrogate fibroblast\u2010dependent inflammatory signaling. These results offer mechanistic support for future translational study of clinical reagents for manipulation of the IL\u201017A pathway in iSGS patients.<\/jats:p><\/jats:sec>","DOI":"10.1177\/0194599818803584","type":"journal-article","created":{"date-parts":[[2018,10,16]],"date-time":"2018-10-16T04:42:31Z","timestamp":1539664951000},"page":"107-115","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":67,"title":["Pathologic Fibroblasts in Idiopathic Subglottic Stenosis Amplify Local Inflammatory Signals"],"prefix":"10.1002","volume":"160","author":[{"given":"Robert J.","family":"Morrison","sequence":"first","affiliation":[{"name":"Department of Otolaryngology\u2013Head and Neck Surgery Vanderbilt University Nashville Tennessee USA"},{"name":"Department of Otolaryngology\u2013Head and Neck Surgery University of Michigan Ann Arbor Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas\u2010George","family":"Katsantonis","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2013Head and Neck Surgery Vanderbilt University Nashville Tennessee USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin M.","family":"Motz","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2013Head and Neck Surgery Johns Hopkins Baltimore Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander T.","family":"Hillel","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2013Head and Neck Surgery Johns Hopkins Baltimore Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. 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